DCPTA与SS3307对烤烟根系脱氢酶活性和叶绿素含量的影响

用DCPTA和唏效唑分别处理K326和G80两个烤烟品种,共设置了4个平行的试验,研究了根系脱氢酶活性、生育期内叶绿素含量动态变化.研究结果表明:用唏效唑300 mg.kg-1和DCPTA 100 mg.kg-1分别处理K326、G80品种后根系脱氢酶活性都显著增强,且品种K326对两种生长调节剂的敏感度强于G80品种;K326经DCPTA 150 mg.kg-1与唏效唑300 mg.kg-1处理后和G80经唏效唑400 mg.kg-1与DCPTA 150 mg.kg-1处理后叶绿素含量均在生育前期增长幅度较大,旺长期都能够达到较高的叶绿素含量且成熟期开始分解,最后达到一个较好的水平.     

金黄色葡萄球菌低分子质量双特异性磷酸酶sLMWDSP的表达、纯化及性质

双特异性磷酸酶是酪氨酸磷酸酶家族中的一员,它参与生物体内信号转导、生长调控、新陈代谢等许多基本的生理活动.金黄色葡萄球菌的低分子质量双特异性磷酸酶sLMWDSP(low molecular weight dual-specificityphosphatase,Staphylococcus aureus)基因从金黄色葡萄球菌cDNA库中克隆,并在大肠杆菌中表达.高纯度的sLMWDSP用亲和层析的方法纯化得到.酶学性质研究表明:sLMWDSP催化对硝基苯磷酸(-pnitrophenyl phos-phate,pNPP)水解反应的最适pH值为6.7,最适温度为35℃,且该酶的活性不依赖于金属离子.磷酸酶通用抑制剂岗田酸(Okadaic acid)、EDTA对sLMWDSP几乎没有抑制作用,但钒酸钠能明显地抑制该酶的活性.sLMWDSP对pSer/Thr以及pTyr的寡肽均有去磷酸化作用,这说明sLMWDSP是一个新的双特异性磷酸酶.     

宁德产小鱼仙草挥发油化学成分及抑菌作用的研究

利用水蒸汽蒸馏法提取宁德产小鱼仙草（Mosla dianthera Maxim）挥发油，得油率为0．50％．应用色谱／质谱／数据系统联用方法分析了其挥发油的化学组成，检测到93个峰，共鉴定了62种化学成分并测定其相对含量，占总含量的98．114％．其中，香荆芥酚（27．229％）、香芹酮（12．326％）、百里香酚（11.027％）、β-石竹烯（7．246％）、柠檬烯（6．209％）、2-甲基-5-（1-异丙基）-乙酰苯酚（5．932％）、间伞花烃（4．982Vo）、葎草烯（4．496％）为主要成分．对7种供试细菌的体外抑菌实验表明，该挥发油对金黄色葡萄球菌（Staphylococcus aureus）、大肠杆菌（Escherichia coli）、藤黄八叠球菌（Sarcina lutea）、枯草芽孢杆菌（Bacillus subtilis）、普通变形杆菌（Proteas vulgaris）、短小芽孢杆菌（Bacillus pumilus）、甘薯薯瘟病原细菌（Fasarium axyssporam）有一定的抑制作用．     

水体氮素对藻类植物的影响

用硝酸铵（NH4NO3）对山西农业大学人工湖水样进行处理，研究水体氮素对藻类植物的影响，用血球计数板法和分光光度法对处理过的水样的主要藻类的数量和叶绿素a含量进行测定，与未经处理过的水样比较分析，结果表明：随着TN浓度的升高，各种藻类的生产力先升后降，当TN浓度为500mg／L时生产力达到最高；小球藻是该实验水体富营养化的优势藻类，栅藻属数量最低；团藻属和小球藻属较硅藻属、栅藻属更能抵抗恶劣环境；实验所观察的4种藻类在TN：6000mg／L的状态下均不能正常生长。     

SiO2掺杂对高温处理后纳米TiO2光催化性能的影响

以钛酸丁酯为原料,用醇盐水解法制备纳米TiO2,用SiO2掺杂纳米TiO2.并对高温处理后的掺杂纳米TiO2光催化活性进行研究。XRD和TEM研究结果表明,SiO2掺杂能有效的提高晶相转变温度,阻止TiO2从锐钛矿型向金红石型的转变和晶粒的长大。实验结果表明,在1050℃高温处理后,SiO2掺杂后的TiO2仍为锐钛矿型纳米晶体。用掺杂纳米TiO2对甲基橙的降解率来表征其光降解性能,SiO2掺杂TiO2在高温处理后依然具有较好的光催化活性,850℃处理后其甲基橙降解率由未掺SiO2时的11%提高到96.48%,晶粒尺寸则由50nm减小到20 nm.     

Phase behavior of supercritical CO2 microemulsion with food-grade surfactant AOT

Supercritical CO2 (scCO2) has drawn wide attentions as a cheap and efficient green solvent replacing the conventional organic solvents. These technological ap- plications increase rapidly especially in food-relative industry, such as extractions of beer h…     

Response of downstream sediment to water resource development in mainstream of the Lancang River

The impact of hydropower cascades of the Lancang River on downstream sedimentation has become one of the key problems in the research of the transboundary water resource development and its possible environmental effects. In this paper, the newest sediment data series spanning 17 years （1987-2003） at Yunjinghong hydrometric station and Chiang Saen hydrometric station, in northern Thailand, are analyzed to reveal these effects. Monthly mean sediment concentration data of the two stations are compared in-phase and yearly series are analyzed by the method of correlation and regression analysis and causality test. The analysis result shows that, though both of them are located at lower reaches of Manwan and Dachaoshan hydropower stations which are already constructed and in use, the sediment responses of the two stations are different under the regulation of the hydropower stations Annual series at Yunjinghong station are positively correlated but do not match well with those at Chiang Saen station. In addition, the results of Granger causality test on the time series also suggest that the series of maximum monthly mean sediment concentration of the Yunjinghong station each year may give cause for the ones of Chiang Saen station, while the series of minimum monthly mean sediment concentration each year and annual mean sediment concentration of Yunjinghong do not evidently give cause for the ones of the other station. Undoubtedly, this discovery will provide new evidences to assess the impacts of cascades construction in mainstream of the Lancang River on the variation of downstream sediment and its transboundary effects.     

紫苏乙醇提取物抗氧化活性研究

以乙醇为溶剂,从紫苏中提取有效成分,采用微生物活性纸片法和烘箱法对紫苏乙醇提取物的抗氧化活性进行研究。结果表明,紫苏乙醇提取物具有较强的抗氧化性,且对油脂食品具有较好的保护作用。     

一株高产抗菌活性物质链霉菌的初步分类鉴定

对一株土壤中筛选出的可以产抗菌活性物质的链霉菌进行了研究，测定了该菌的抗菌谱，发现该菌株对多种植物病原性真菌及细菌有拮抗作用，但对植物病原性真菌的抑制效果低于植物病原性细菌.通过形态特征、培养特征、生理生化特性及抑菌谱等系列比较，发现该链霉菌菌株与链霉菌属的黑胡桃链霉菌(Streptomyces nogalater)的特征基本相符；通过16S?rDNA序列比对，发现该菌株16S?rDNA与黑胡桃链霉菌的16S?rDNA同源性达99％.根据多项分类原则和系统进化树的构建分析，将该菌株暂归入黑胡桃链霉菌类.