6,7-dichlorobenzo[de]isochromene-1,3-dione的合成新方法

本文报道了6,7-dichlorobenzo[de]isochromene-1,3-dione的合成新方法。该路线简单易行,后处理方便,且产率较高,有较高的实用价值。     

基于DM643的交通信息采集系统

为实时、准确获取道路交通信息,满足当前智能交通系统的应用需求,设计并实现了一种基于高性价比DSP硬件平台的嵌入式交通信息采集系统。给出了视频输入输出接口／电路和网络通讯接口电路的设计,并基于DSP／BIOS开发了系统驱动程序。实验结果表明,该系统具有很好的稳定性和实时性。     

灵芝免疫调节蛋白LZ-8基因的克隆与表达

灵芝能够调节和增强机体免疫力,具有极高的药用价值;LZ -8蛋白是最早被分离研究的免疫调节蛋白,它不仅是灵芝生命活动的重要物质而且具有独特免疫原性.实验采用CTAB法提取灵芝基因组DNA,PCR扩增全长lz -8基因;利用XbaⅠ,XhoⅠ位点将lz -8编码基因片段克隆到原核表达载体pET -28a中,构建原核重组表达质粒pET -lz8,转化BL21(DE3)并进行SDS - PAGE及Western - blot分析.结果表明:克隆的lz -8基因与GenBank报道的序列同源性达到87.5%～100%;重组菌株经IPTG诱导后,lz -8基因得到了高效表达,目的蛋白表达量可达菌体总蛋白的36.52%.     

CCK-8法和MTT法检测人前列腺癌PC3细胞活性的最佳条件比较研究

以人前列腺癌PC3细胞作为研究对象,探讨了CCK-8法和MTT法的最佳实验条件。CCK-8的最佳检测波长为450nm,最佳检测时间为加入CCK-8试剂后4h,适宜细胞数量范围为8×10^2-1×10^5个。在检测抗肿瘤药物阿霉素（ADM）对PC3细胞的增殖影响实验中,发现CCK-8法测得数据的线性相关性优于MTT法,并且数据偏差较MTT法小。实验结果表明CCK-8法较MTT法检测的灵敏度高,准确性好,是一种优于MTT法的检测细胞增殖活性的方法。     

nRF905的LED屏无线通信设计与实现

针对采用芯片nRF905的LED屏无线通信,分别给出了上位机和下位机的系统框图,分析了系统的功耗,比较了无线模块和串口通信的通信速率,验证了系统的可行性,设计了串口通信协议,为保证数据质量,设计了数据通信协议,针对串口数据的nRF905分包转发,设计了无线芯片通信协议,例举了状态机的五种状态,介绍了状态间的转换条件,巧妙地编程设计了通信数据的定时器检查,论述了基于状态机的嵌入式单片机软件编程。     

人类8型疱疹病毒K1基因真核表达载体的构建

为构建人类8型疱疹病毒(Human herpesvirus 8,HHV-8)K1基因的真核表达载体,采集卡波氏肉瘤(Kaposi's sarcoma,KS)患者血清,采用柱式病毒DNA抽提试剂盒抽提血清中的病毒DNA,采用套式PCR技术检测HHV-8的特异性片断KS330233以判断是否得到了HHV-8 DNA,采用PCR技术扩增HHV8 K1基因并纯化回收,酶切、连接入真核表达载体pcDNA3.1,转化大肠杆菌DH5α,挑取阳性克隆pcDNA3.1/HHV8-K1扩增后,提取质粒,进行双酶切和DNA测序鉴定.结果显示采集了经典型KS血清3例,提取了血清HHV-8DNA,扩增了特异性片断KS330233,测序结果正确提示我们得到了HHV8 DNA,扩增了HHV8 K1基因,构建了pcDNA3.1/HHV8-K1真核表达载体,经限制性内切酶酶切鉴定及测序分析证实了其序列的正确性.由此可知:成功构建了pcDNA3.1/HHV8-K1真核表达载体,为进一步研究该基因的功能奠定良好的实验基础.     

基于语音的微机控制报警器

提出了以8031单片微机和ISD2590语音芯片为核心的微机控制报警器的设计方法，介绍了ISD2590的特点和使用方法，阐述了系统的硬件设计和软件设计。     

A New Implementation of the Post-Stage Tasks of Motion Estimation Using SIMD Architecture

IntroductionThe MPEG2 [1] video compression standard is oneof the key technologies for applications such asdigital broadcasting,storage media,etc.Recently,some single chip video encoders havebeen developed for the MPEG2 standard. Thedevelopment of hardware architecture suitable forsystem integration is the key to single chipimplementation.Motion estimation/compensation is the keypart of a single MPEG2 video encoder chip. It hasa great impact on the chip size,the powerdissipation,and the …     

单片机控制多功能显示系统的设计与实现

采用单片机作为系统核心控制部件，该系统具有利率显示、日历时钟、字显示功能。作为DS12887的应用，介绍了它与8031单片机构成的时钟系统电路。     

Protective effect of Shigyaku-to,a traditional Chinese herbal medicine,on the infection of herpes simplex virus type 1 (HSV-1) in mice

The antiviral activity of Shigyaku-to (TJS-109), a traditional Chinese herbal medicine, was investigated in mice infected with herpes simplex virus type 1 (HSV-1). TJS-109 is a combination of the medicinal plant extracts fromZingiberis siccatum rhizoma,Aconiti tuber andGlycyrrhizae radix in a specific proportion. Mice infected with a 10 LD₅₀ dose of HSV-1 were treated with TJS-109 orally at doses of 1.25 to 20 mg/kg 2 days before, and 1 and 4 days after the infection. The treated groups had 80% (1.25 mg/kg), 40% (5 mg/kg) and 23% (20 mg/kg) mortality rates 25 days after the infection as compared with a 100% mortality rate in control mice treated with saline. When HSV-1 infected mice (recipients) received CD8⁺T cell fractions derived from spleens of mice treated with TJS-109 (donors), 70% of recipients survived, as compared with 0% survivors in the groups of mice treated with saline, B cell fractions, CD4⁺ T cell fractions or macrophage-enriched fractions prepared from the same donors. TJS-109 did not show any virucidal activities against HSV-1 or any virostatic activities on the growth of HSV-1 in Vero cells. These results suggest that TJS-109 protected mice exposed to lethal amounts of HSV-1 through the activation of CD8⁺ T cells.