Acylphosphatase overexpression triggers SH-SY5Y differentiation towards neuronal phenotype

An acylphosphatase (AcPase) overexpression study was carried out on SH-SY5Y neuroblastoma cells, using a green fluorescent fusion protein (AcP-GFP), with GFP acting as a reporter protein. The cellular proliferation rate was significantly reduced by overexpression of AcPase by a factor of ten. In contrast, clones transfected with two inactive AcPase mutants showed a growth rate comparable to control cells. This suggests that AcPase catalyzes the proliferative down-regulation. AcPase-overexpressing clones showed a physiological mortality rate as assessed by an MTT reduction test and by evaluation of necrotic markers. DNA fragmentation analysis and assays of caspase-3 and poly (ADP-ribose) polymerase (PARP)-active fragments showed no evidence of any apoptotic pattern. AcPase overexpression led to a marked increase in PARP activity as well as Bcl-2 content; these are commonly up-regulated during differentiative processes in neuronal cells. In fact, the typical differentiation marker, growth-associated-protein 43, was significantly up-regulated. Microscopic observations also showed a clear increase in the differentiative phenotype in AcPase-overexpressing cells. Our results clearly show that AcPase plays a primary causative role in neuronal differentiation.Received 3 May 2004; accepted 25 May 2004     

由新型TOP224Y构成12V／40W开关电源

单片开关电源正显示出强大的生命力,并以其优良的性能倍受人们青睐．中阐述了利用被人们誉为“顶级开关电源”的反激式单片开关电源芯片TOP224Y构成12V、40W的开关电源电路设计。     

超稳Y-沸石催化合成乙酰乙酸乙酯缩酮

用脱铝超稳Ｙ－沸石作催化剂合成了乙酰乙酸乙酯与乙二醇缩酮和丙二醇缩酮,其产率可达到８０％和９６％,考察了催化剂组成和反应时间对反应的影响规律,发现产物比反应物更易在催化剂表面吸附,对反应机理作了讨论。     

电脑千年虫问题的解决方法分析与实践

千年虫问题是电脑系统以２位存储年份而产生的在２０００年后不能正确识别年份的问题,解决千年虫问题的根本就是解决年份的存储和识别问题,本文综合分析了目前常用的解决千年虫问题的几种方法,及其特点、复杂性及成本,最后介绍了我们在实际解决应用系统千年虫问题上的实践。     

某电子设备接触电流偏大的原因分析及解决方法

文章从接触电流成因、导致接触电流偏大的因素等2个方面对接触电流偏大的原因进行了理论分析,结合某电子设备的实例详细论述了该问题的解决过程,并最终给出了切实可行的整改措施,提供了相关问题的解决途径。在文章最后提出了＂过度设计＂的概念,指出了它的危害。     

DMSO中Y—Co合金膜的电化学制备

稀土合金作为功能材料，广泛地应用在磁、光、核及超导材料领域．目前一般采用高温或低温熔盐电解及真空镀的方法来制备稀土及其合金材料．如果能在室温下通过电沉积的方法制备稀土合金，则便于控制合金组成，可大大提高它的功效和扩大应用领域．但是，氢的析出使稀土很难...     

拟南芥钙依赖蛋白激酶CPK11与ABA响应元件结合因子ABF4相互作用的研究

为研究钙依赖蛋白激酶CPK11参与ABA信号通路的方式,本文利用体外酵母双杂实验(Y2H)以及体内双分子荧光互补实验(BiFC)分析CPK11与ABA响应元件结合因子(ABA-responsive element binding factors, ABFs)ABF4之间的关系.酵母双杂交实验表明CPK11与ABF4存在体外相互作用,双分子荧光互补实验表明CPK11与ABF4存在体内相互作用.以上实验共同证明CPK11与ABF4存在直接相互作用.作为CPK11的同源蛋白CPK4与转录因子ABF4在植物体内也存在相互作用.以上实验表明CPK11及其同源蛋白CPK4可能通过与转录因子ABF4相互作用从而参与钙离子介导的ABA信号通路.     

2000年问题与计算机教学

2000年问题说到底是在把资源无限的人脑中的数字转换到资源有限的计算机系统中的数字时使用方法不当而造成的问题,因而并不是一个特别的问题,而是一个普遍的问题。本文试图从教学的角度讨论怎样教好学生从本质上认清计算机系统中的信息、特别是数字的定义和处理,使学生能更加深刻地理解计算机和计算机系统,避免在今后的工作中再犯类似2000年的问题的错误。     

内蒙古葱属一新变型及其花粉形态

分类号Ｑ９４９文献标识码Ｂ文章编号１００１－８７３５（１９９９）０１－００６２－６３白花蒙古韭新变型ＡｌｉｕｍｍｏｎｇｏｌｉｃｕｍＲｅｇｅｌｆ．ａｌｂｉｆｌｏｒｕｍＹ．Ｂａｏｆ．ｎｏｖ．Ａｔｙｐｏｄｉｆｅｒｔｐｅｒｉａｎｔｈｉｓｅｇｍｅｎｔａａｌｂａ...     

Occurrence,distribution and nature of neuropeptide Y in the rat pancreas

Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.