基于聚乙烯亚胺功能化石墨烯的高灵敏电化学阻抗传感方法用于血清中mircoRNA-155检测

建立血清中癌症标志物microRNA-155的高灵敏电化学检测方法.采用一步法制备了聚乙烯亚胺功能化的石墨烯,通过静电相互作用制备了金纳米粒子功能化的石墨烯复合物.将该复合物用作电极基底材料,基于金纳米粒子与DNA捕获探针上的SH基团之间形成的强烈的S-Au键可将DNA捕获探针固定到电极表面.通过DNA捕获探针对microRNA-155的特异性识别作用可特异性地捕获待测样品中的microRNA-155分子.利用生物分子对电极表面电子传递的阻碍作用,从而实现对microRNA-155分子的电化学阻抗传感器的构建.在最优条件下,该传感器的阻抗值与microRNA-155的浓度的对数值在1～1 000 nmol/L浓度范围内呈现良好的线性关系,检出限为0. 51 nmol/L(S/N=3),可实现实际血清样品中的microRNA-155的灵敏快速检测.     

寡核苷酸芯片制备及其杂交条件的优化

探讨了2种不同硅烷化试剂3-氨基丙基三甲氧基硅烷、三甲氧基丙基二乙烯基三胺及其不同处理时间对寡核苷酸探针固定效率的影响.结果表明3％的3-氨基丙基三甲氧基硅烷的95％丙酮溶液处理2h,然后用5％戊二醛溶液处理1 h,可以获得很好的杂交信号.对比polyT15空间子,含有相同寡核苷酸序列的PEG6、PEG12空间子能使杂交信号增强6、8倍.在以pH值9-11的碳酸盐缓冲液为点样液时,杂交信号随着pH值的逐渐升高而略有下降.寡核苷酸探针的浓度和荧光标记样品的浓度影响杂交结果,当寡核苷酸探针的浓度为25μmol、所用长度为110bp的荧光标记的PCR产物为2μL,经杂交液6×SSEPT稀释至10μL时,可以获得很好的杂交信号.     

A tetrairon（Ⅲ） complex recognizing protein structures via a hydrolytic pathway

There might be significant differences in the rate and efficiency in the metal complex-mediated hydrolytic reactions of proteins belonging to the different structural patterns. The tetrairon（Ⅲ） complex [Fe4（NTB）4 （μ2-O）2（μ4-Suc）]^6＋, as a promoter in protein hydrolysis, is sensitive to α-helices in proteins, indicating that some metal complexes, as artificial proteolytic agents, could be used as a new hydrol） tic probe of protein structures.     

高师实践性教学改革的探讨

从教学目标、教学内容、教学方法、教学手段、组织方式及考核评价等方面对高等师范院校实践性教学的改革进行探讨 ,并提出改革设想     

薄膜磁阻效应计算机控制自动测试

采用单片机8031模拟三角色波输出而产生超低频扫场电源，克服阻容元件线差波形不稳定，用8位单位片机与16位数模板转换器进行接口，给出了控制系统，D／A转换，恒流和功率驱动电路以及软件设计，以四探针，六探针法测量，实现对薄膜磁阻效应的自动测试。     

Vorticity Measurements Using a 6-Sensor Hot-Wire Probe in a Tangentially Fired Furnace

Vorticity, which represents the rotation of a fluid element, is an important characteristic of turbulence. Various methods have been used to measure vorticity. A hot-wire/hot-film anemometer (HWA) was used here to measure the vorticity in turbulent flows. The velocity components and their partial derivatives were simultaneously measured with a new 6-sensor hot-wire (HW) probe assuming ideal yaw and pitch factors with Jorgensen's expression and Taylor's hypothesis to analyze the data. The accurate 6-sensor hot-wire probe results for the velocity field were used to determine the velocity gradients and, therefore, the vorticity vector field. The data was measured in an isothermal model of a tangentially fired furnace. The experimental results in the tangentially fired furnace agree with numerical results.     

Microstructures and Magnetic Properties of Fe-Zr-B Based Nanocrystalline Alloys

We have studied the partitioning of B in Fe -14B and of Zr, B, Si, Cu and Au in FeZrB nanocrys-talline soft magnetic alloys by using atom probe field ion microscopy (APFIM) and high resolution electron microscopy (HREM) in atomic or nano-metric scales and'the effect of Si, Cu, andAuaddition on the magnetic properties. For Fe- 14B after annealing, a -Fe particles with diameters of about 50-100 nm formed and they contain high supersaturated B. For Fe - 7Zr-3B -1 Cu amorphous alloy, it was found that Cu atoms formed clusters prior to the crystallization reaction. During the crystallization, Cu-enriched clusters provided nucle-ation sites for a -Fe phase so that the nucleation density of a -Fe phase increased. For Fe - 7Zr -5B- 1Au amorphous alloys, Au clusters were observed during the nucleation and growth stage of primary a -Fe and a -Fe did not form at the sites of Au cluster. Hence Au does not enhance the nucleation density of a -Fe particles. For Fe-7Zr-4Si-2B alloys it was found that Si atoms are reje     

为《语言文字散论》所作的序

<语言文字散论>能关注学术研究前沿,探讨重大问题;能着力述评,抒发已见;并能做到来自教学,服务教学,不失为一部较好的论文集.     

Pre-aging on early-age behavior and bake hardening response of an Al-0.90Mg-0.80Si-0.64Zn-0.23Cu alloy

Pre-aging on early-age behavior and bake hardening response of an Al-0.90Mg-0.80Si-0.64 Zn alloy was investigated by differential scanning calorimetry(DSC), high resolution transmission electron microscopy(HRTEM), 3-dimensional atom probe(3DAP), Erichsen test and tensile test. The results indicated that pre-aged alloy exhibited excellent formability and bake-hardening response, while bake hardening response was poor in samples with natural aging. Clustering behavior during natural aging was inhibited by pre-aging. Numerous GP zones formed in pre-aged samples. GP zones were the nuclei of β′′ precipitates or directly transformed β′′ phases during paint baking process. A large number of β′′ phases were observed in pre-aged samples after paint bake treatment. There was no sign to indicate that β′′phase precipitated in natural aged samples after bake hardening treatment.     

基于二氟荧光素内酰胺的一氧化氮分子荧光探针的合成及应用

合成了基于二氟荧光素内酰胺开环反应的一氧化氮分子荧光探针并对其结构进行了表征。二氟荧光素内酰胺本身无荧光,当与NO反应后开环水解生成二氟荧光素,在λex=484nm光激发下产生荧光且最大发射波长λem=514nm。在PH〉4．8的体系中二氟荧光素内酰胺与NO反应后荧光强度达最大且稳定。在H2O2、·OH、O2-、NO2-、ONOO-存在下,荧光探针对NO表现出很高的选择性。以NOC13为NO释放剂,荧光强度随NOC13浓度的增加而增大,可实现对NO的直接检测。