职业学校素质化教育探讨

职业学校应在实施素质教育，加强实验、实习教学，培养高等应用型人才上下功夫，以突出职业学校与普通院校的特色。     

Progress in materials and technologies for ultrahigh density data storage

With the development of information superhighway, nanometer-scale data storage has been proposed and attracted great interest in recent years. This article reviews the research achievements in this field, and especially focuses on the materials for data recording by using an atomic force microscope (AFM) and scanning tunneling microscope (STM).     

新形势下对高校学生工作思路的探索

从提高学生全面素质，培养创新人才的目标出发，结合工作实践，阐述了高校学生工作思路，即以高尚的思想教育人，以文明的行为引导人，以优良的学风鼓舞人，以科学的管理规范人，以丰富的活动陶冶人，以优质的服务奉献人。     

西方市场社会主义者对微观激励机制的建构

当代西方市场社会主义者探索了市场社会主义微观激励和创新动力的获得问题 ,明确指出了虽没有私有制但只要有竞争也可以获得微观激励和创新的动力 ,并为此设计了多种微观激励的新机制。他们的这种探索与建构 ,对中国建立社会主义市场经济条件下的现代企业制度具有重要的参考价值     

心理咨询中的不确定性因素探析及对策

心理咨询面临着一系列不确定性因素.探析了不确定性因素的类型及产生原因,并为解决这个问题提出了相应的应对策略.     

基于磁性荧光双探针基础上的2,4-二氯苯氧乙酸残留的快速免疫检测体系的建立(英文)

建立了一个基于磁性荧光双探针基础上的免疫快速检测体系,以实现液相中快速检测食品中2,4-二氯苯氧乙酸(2,4-D)残留.该体系将2,4-D抗体结合Fe3O4@SiO2-NH2得到的复合物作为磁性探针和固相载体,2,4-D-OVA被标记CdTe@SiO2-NH2作为荧光探针以产生荧光信号,通过荧光探针与磁性探针复合物与2,4-D抗体竞争结合实现免疫快速检测.探讨了荧光探针最佳优化条件,在p H值8.2,2,4-D-OVA加入量为500μL,偶联时间为70 min时,偶联得到的荧光信号最强,双探针检测后得到该检测体系最低检测限为3.55×10-8.得到金磁、量子点荧光双探针免疫系统,绘出标准曲线,得到最低检测限达3.55×10-8.该检测体系与传统ELISA方法相比,可以大大缩短检测时间,放大检测信号.     

对阴、阳离子选择性识别的钳型菲啰啉荧光探针

2,9-二甲基-1,10-邻菲啰啉与2,4-二羟基苯甲醛在乙酸酐中缩合反应得到2,9-（E,’E）-双（2’,4’-二（乙酰氧基）苯乙烯基）-1,10-菲啰啉（探针a）;再将其进一步水解得到2,9-（E,’E）-双（2’,4’-二（羟基）苯乙烯基）-1,10-菲啰啉（探针b）.探针经^1 H NMR、^13 C NMR、IR、MS表征,为钳型对称大共轭结构,发光性能良好.探针a、b均对Cu^2＋、Ag^＋有荧光猝灭作用;探针b由于分子中含有羟基结构而选择性识别阴离子F^-、AcO^-,为一种双功能离子探针.光谱滴定测出探针a、b与金属离子的作用比为2∶1;探针b与阴离子的作用比为1∶1,作用稳定常数均达104 L·mol^-1.作为检测特定离子的荧光探针具有良好的分析参数.     

Investigation of self-assembled protein dimers through an artificial ion channel for DNA sensing

Artificial nanopores have become promising tools for sensing DNA. Here, we report a new technique for sensing DNA through a conical-shaped nanopore embed- ded in track-etched polyethylene terephthalate （PET） membrane. Two different streptavidin-conjugated mono- valent DNA probes were prepared that can bind to two distinct segments （at either end） of the target DNA. The size of target DNA-linked to the two streptavidin-conju- gated monovalent DNA probes is double that of the indi- vidual probes. By precisely controlling the tip diameter of the conical nanopore embedded in the PET polymer, events due to the translocation of the streptavidin-conjugated monovalent DNA probes through the nanopore can be fil- tered and purposely undetected, whereas the current pulses due to the translocation of the target DNA-induced self- assembled complexes can be detected. The two streptavi- din-conjugated DNA probes cannot be linked by multi- mismatched DNA. Therefore, multi-mismatched （non- specific） DNA will not induce any current pulse signatures. The current pulse signatures for the self-assembled com- plex can be used to confirm the presence of the target DNA. The size-dependent detection of self-assembled complexes on the molecular level shows strong promise for the detection of biomolecules without interference from the probes.     

The influence of probe lift-up height on CNT electrical properties measurement under EFM DC mode

Nowadays, one of the bottlenecks which hinder the development and application of carbon nanotube （CNT） nano device is that no pure semiconducting CNT （s-CNT） or metallic CNT （m-CNT） can be obtained, and for solving this problem scientists proposed some methods on preparation or separation, but all the results still should be detected and feedback to the process for further improving the preparation and separation methods. Thus, it is very important to measure and distinguish the electrical properties of CNT. For that, scientists proposed a method to measure CNT electrical properties based on DC elec- trostatic force microscope （EFM） mode, which distin- guishes m-CNT from s-CNT according to different scan line shape to CNT with different electrical properties. But, we discovered that the probe lift-up height will seriously affect the shape of the scan line, which makes this method not reliable in distinguishing m-CNT from s-CNT. In this paper, the authors deeply researched the influence of probe lift-up height and also gave corresponding theoretical analysis and explanation, which will greatly improve the method of detecting CNT electrical properties by EFM.     

乙酰靛玉红对SDS临界胶束浓度的测定

本文以乙酰靛玉红为探针采用荧光光谱法对阴离子表面活性剂十二烷基磺酸钠(简称SDS)的梯度浓度下的荧光强度进行了测定.结果表明,当SDS浓度为8.20 mmol·L-1时,乙酰靛玉红发射峰位置592 nm处的荧光强度发生了明显的变化,此突变点对应的SDS浓度值与其临界胶束浓度文献值一致,表明将乙酰靛玉红作为荧光探针测定SDS的CMC是可行的.