Expression and immunolocalisation of odorant-binding and chemosensory proteins in locusts

We have identified, cloned and expressed a new chemosensory protein (CSP) in the desert locust Schistocerca gregaria belonging to a third sub-class of these polypeptides. Polyclonal antibodies stained a band of 14 kDa, as expected, in the extracts of antennae and palps of the adults, but not in the 4th and 5th instars. In the related species Locusta migratoria, instead, the same antibodies cross-reacted only with a band of apparent molecular mass of 35 kDa in the extract of 1st–5th instars, but not in the adults. The recombinant protein binds the fluorescent probe N-phenyl-1-naphthylamine, but none of the compounds so far reported as pheromones for S. gregaria. The expression of the odorant-binding protein (OBP) and of CSPs of sub-classes I and II was also monitored in antennae, tarsi, palpi, wings and other organs of solitary and gregarious locusts in their nymphal and adult stages. OBP was found to be antenna specific, where it is expressed at least from the 3rd instar in both solitary and gregarious locusts. CSPs, instead, appear to be more ubiquitous, with different expression patterns, according to the sub-class. Immunocytochemistry experiments revealed that OBP is present in the sensillum lymph of sensilla trichodea and basiconica, while CSP-I and CSP-III were found in the outer sensillum lymph of sensilla chaetica and in the sub-cuticular space between epidermis and cuticle of the antenna. Sensilla chaetica on other parts of the body showed the same expression of CSP-I as those on the antenna.Received 11 Janury 2005; received after revision 21 February 2005; accepted 18 March 2005X. Jin and A. Brandazza contributed equally to this work.     

忧患意识与文心诗心的比较——《谈艺录》、《管锥编》对莎士比亚作品的引述

钱钟书所著《谈艺录》、《管锥编》中共有３６次提到莎士比亚及其作品。钱先生对莎作的阐释，意义在于通过莎作阐明中西文化、艺术、文学之间的异同和共有的文心诗心，对于中国莎学研究具有方法论方面的意义。     

积极的财政政策与中西部的崛起

党的十六届三中全会提出了"五个统筹"的发展观,实施积极的财政政策应向中西部倾斜。财政要充当规划、发动、调节中西部开发中基础设施建设的主导力量;要在构建中西部大的产业结构框架(如"西气东引"西电东输"等工程)方面发挥更大的作用;要进一步加大中央对中西部地区的财政转移支付制度,以支持中西部地区的产业升级和骨干企业的技术改造。同时,要探寻财政资金运作的新方式,引导其他投资及时跟进,最终实现中西部地区的崛起和全国共同富裕的战略目标。     

先秦时期铜陵地区的硫铜矿冶炼研究

中国古代何时利用硫铜矿冶炼金属钢一直是引人注目的问题．作者借助于定量化学分析、光谱分析、金相分析和扫描电镜等手段，对铜陵地区出土的先秦时期的钢锭和炼渣进行了全面检测，并经过实地考察及文献考证，认为这地区至迟在西周晚期就已经利用硫铜矿冶炼金属铜了。     

对BIV－Gag和Env抗原特异性抗体的免疫检测

本文用高效表达载体表达出的融合ＢＩＶ核心蛋白（ＴｒｐＥ－Ｇａｇ）和外膜蛋白（ＴｒｐＥ－Ｅｎｖ）作为ＷｅｓｔｅｒｎＢｌｏｔ检测的抗原，分别对Ⅰ、Ⅱ两口岸进口奶牛及后代进行了流行病学调查，发现用外膜蛋白检测阳性率比核心蛋白阳性率略高，进一步的追踪调查表明核心蛋白的抗体水平在减弱的同时外膜蛋白的抗体有加强的趋势。     

我国最早的道教炼丹实物——绵阳双包山汉墓出土金汞合金的初步研究

四川绵阳双包山2号西汉墓出土一块银白色膏状金属,据检测,它主要由液态汞和金汞合金颗粒组成,即所谓的金汞齐;结合文献分析,可知它是我国迄今为止发现的最早的方士的炼丹实物;同墓出土的银缕玉衣、木璧、人体经脉漆雕、陶质丹丸等文物是炼丹实物的旁证。     

以特色图书馆建设提高西部高校图书馆的核心竞争力

介绍了图书馆核心竞争力的概念,分析了西部高校图书馆提升核心竞争力的困境,探讨了西部高校图书馆核心竞争力的构建。     

产业集群增长与西部工业竞争力的提升

产业集群是市场经济条件下工业化进行到一定阶段后的丛然产物，是现阶段产业竞争力的重要来源和集中体现。由于集群内产业结构趋同，企亚关联戴应弱，区内支持性产业发展不健全，具有地方特色的集群文化建设滞后，创新能力不强，西部工业在全国处于弱势竞争地位。确立地方产业集群增长战略，通过政府的引导促使当地关联企业走向产业集群的路径对提升西部工业竞争，加快推进其工业化进程是一项既现实又具有重要意义的课题。