用P11柱一步纯化色氨酸阻遏蛋白质

利用携带有克隆色氨酸阻遏蛋白基因的pJPR2质粒的大肠杆菌菌株,经过细菌培养、扩增及一系列生化方法,得到色氨酸阻遏蛋白的粗品,然后利用磷酸纤维素P11柱分离纯化得到单一的色氨酸阻遏蛋白,经SDS聚丙烯酰胺凝胶电泳鉴定和pRK9质粒中trpP/O片段保护实验证明,分离到的色氨酸阻遏蛋白是纯的,并具有生物活性。该方法和思维的建立,为研究核酸结合蛋白质的结构与功能提供了基础。     

解决机房卸载Oracle 11g及其安装问题的方法

在机房中安装Oracle 11g,由于电脑太多,不方便逐台安装,因此现在的安装方式是：先制作母盘,再进行硬盘对拷,但是对拷后的机器oracle不能直接使用,必须修改一系列设置.同样,如果Oracle 11g删除不干净,重新安装将会出错.本文将详细介绍如何在Windows XP系统下完全卸载和克隆安装Oracle 11g的方法.     

Pheromone components of the female elephant hawk-moth,Deilephila elpenor,and the silver-striped hawk-moth,Hippotion celerio

By means of gas chromatographic and mass spectroscopic methods, and combined GC-electroantennogram and electrosensillogram techniques, (E)-11-hexadecenal and (10E, 12E)-10,12-hexadecadienal [(E,E)-bombykal is also the main constituent of the pheromone of the silver-striped hawk-mothHippotion celerio. The biological activity of the substances was demonstrated with electroantennogram and single cell recording, and the physiological efficacy of the different hexadecadienal isomers compared.Pheromones, 79; as Pheromones, 78 is taken: Wu, Cai-Hong, and Bestmann, H. J., Chinese Science Bulletin34 (1989) 1475; pheromones, 77: Attygalle, A. B., Steghaus0Kovac, S., Ahmed, V. U., Maschwitz, U., Vostrowsky, Ol, and Bestmann, H. J., Naturwissen-schaften78 (1991) 90.     

Human and rodent type 1 11β-hydroxysteroid dehydrogenases are 7β-hydroxycholesterol dehydrogenases involved in oxysterol metabolism

Interconversion between cortisone and the glucocorticoid receptor ligand cortisol is carried out by 11-hydroxysteroid dehydrogenase (11-HSD)isozymes and constitutes a medically important example of pre-receptor control of steroid hormones. The enzyme 11-HSD type 1 (11-HSD1) catalyzes the conversion of cortisone to its active receptor-binding derivative cortisol, whereas 11-HSD type 2 performs the reverse reaction. Specific inhibitors against the type 1 enzyme lower intracellular levels of glucocorticoid hormone, with an important clinical application in insulin resistance and other metabolic disorders. We report here on the in vitro oxysterol-metabolizing properties of human and rodent 11-HSD1. The enzyme, either as full-length, membrane-attached, or as a transmembrane domain-deleted, soluble form, mediates exclusively conversion between 7-ketocholesterol and 7-hydroxycholesterol with similar k_cat values as observed with glucocorticoid hormones. Thus, human, rat, and mouse 11-HSD1 have dual enzyme activities like the recently described 7-hydroxysteroid dehydrogenase/11-hydroxysteroid dehydrogenase from hamster liver, but differ fundamentally from the latter in that 7-OH rather than 7-OH dehydrogenase constitutes the second activity. These results demonstrate an enzymatic origin of species differences in 7-oxysterol metabolism, establish the origin of endogenous 7-OH cholesterol in humans, and point to a possible involvement of 11-HSD1 in atherosclerosis.Received 30 December 2003; received after revision 16 February 2004; accepted 16 February 2004     

星际HⅡ/H_2O脉泽的一种激发机制

本文对于星际HⅡ/H_2O脉泽提出一种重要的激发机制——通过紫外辐射吸收的水的选择性预离解机制,并用它解释了HⅡ/H_2O脉泽能级布居反转的原因,预言了一系列水脉泽跃迁的存在,证明了它与各种天文条件的自洽.     

第十一届全运会市场开发SWOT分析

以2008年北京奥运会的成功举办以及全球经济危机产生的影响为社会背景,从＂奥运效应＂、独特的齐鲁文化为举办优势（strengths）,缺乏大赛举办经验、城镇居民可支配性收入较低、全运会赛事负面影响为劣势（weakness）,山东国际孔子文化节、建国六十周年庆典、我国体育赛事改革为历史机遇（opportunities）,以及经济危机、2010年亚运会举办、地震捐赠援建为挑战（threats）进行分析,研究认为：第十一届全运会市场开发,山东省应借奥运之势全力打造全运会品牌 大力开发群众市场 立足国内企业,有所为有所不为.