Structural analysis of the promoter of tomato 1-aminocyclopropane-1-carboxylate synthase 6 gene(Le-ACS6)

Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato,Le-ACS6,a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening,is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control,we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase(LUC) reporter gene driven by a 5′-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between -347 and -266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from -347 to -266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from -347 to -266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.     

电压互感器爆裂原因剖析及防范措施

本文针对神火一电厂6kV系统电压互感器自投产以来发生的几次损坏现象,简要阐述电磁谐振产生的原因、危害及防范措施,同时也提出针对此类故障应从哪些方面入手的意见,希望能对一些单位有所借鉴。     

二阶双曲型方程的C^0-连续一次有限元法

对于二阶常微分方程初值问题,构造了C0-连续一次有限元法计算格式,通过直接计算的方法证明了误差估计,并利用数值实验验证了理论分析结果.对于二阶波动方程,构造了C0一次有限元法的计算格式,证明了解的存在惟一性,利用数值实验验证了该方法的有效性.     

下一代互联网的发展与展望

本文评述了下一代互联网在国际国内的发展现状、一些关健技术及对其的展望。     

三水合硝酸钇与冠醚18C6配合行为的研究

用改进的半微量相平衡方法研究了Y(NO_3)_3·3H_2O-18C6-CH_3OH三元体系在25℃时的溶解度,测定了饱和溶液的折光率。结果指出,在该体系中只生成一种化学计量的配合物,Y(NO_3)_3·18C6·3H_2O·1.5CH_3OH。考查了相平衡过程中水的行为。系统地比较了三水硝酸化与冠醚18C6在不同溶剂中的配合行为。考查了配合物组成与后处理条件的关系。分离中得了组成为Y(NO_3)_3·18C6·3H_2O的固态配合物,通过化学分析、红外光谱分析、DTG、TG及DSC研究了配合物的组成和性质。测得配合物的脱水和快速热分解过程的焓变值,求得了它们的表观活化能。在常量情况下,考查了配合物的热失重情况。     

马来蕉离体组织培养及其规模化生产

采用马来蕉（Musa acuminata cv．Berangan）的吸芽苗顶端生长组织作为外植体,建立香蕉无病毒体系,进行离体组培培养的研究,经诱导分化、增殖、生根而完成其规模化生产流程。诱导分化培养基为AMS＋6-BA2～3mg／L＋NAAO．01mg／L＋VC50mg／L＋柠檬酸100mg／L,诱导率为90％～96‰增殖培养基为AMS＋6-BA0.3～1．0mg／L＋NAA0．01mg／L,增殖率为2．7～4;壮苗、生根培养基为AMS＋NAA0．01mg／L,生根收获率为60％～95％,生根率为100％。马来蕉对6-BA都比较敏感,6--BA浓度高了易产生泡叶茅、胚状芽等变异芽。     

对脉冲双星PSR1913＋16引力辐射计算的一个注解

本文在牛顿的万有引力定律与爱因斯坦的狭义相对论之间找到一个理论结合点,使质电类比更具理据,以此对陈永明的脉冲双星PSR1913＋16引力辐射的计算提供一个注解。     

Cyclophosphamide-impaired regulation of hepatic heme metabolism

Summary Im male rats hepatic cytochromes b₅ and P-450 were reduced at different times after treatment with cyclophosphamide (CP) (200 mg/kg i.p. for 3 days). In contrast, microsomal heme did not change until 48 h after the last dose of CP, leading to accumulation of heme in a non-cytochromal form. Parallel to the above changes the heme metabolism showed derangement: -aminolaevulinate synthase, the rate-limiting enzyme in heme synthesis, was depressed and heme oxygenase, the enzyme which catalyzes the oxidative degradation of heme, was increased.     

Antiviral activities of anthraquinones,bianthrones and hypericin derivatives from lichens

The antiviral activities of some naturally occurring anthraquinones bianthrones, and hypericin derivatives were compared by the end-point CPE (viral cytopathic effects) method and plaque assays. Under optimal conditions of exposure to light, hypericin, 7,7-dichlorohypericin and 5,7-dichloroemodin exhibited strong inhibitory activity against HSV-1 (herpes simplex virus type 1) in both assays. Partial inactivation of the virus was shown by emodin, 7-chloroemodin and 7-chloro-1-O-methylemodin; the bianthrones and other anthraquinones were found to be inactive. Antiviral activity appeared to be, positively correlated with increasing substitution of chlorine in the anthraquinone structure. In the absence of light, only hypericin and 7,7-dichlorohypericin displayed detectable activity.