Increase of Expression Levels of Reporter Gene in Transgenic Tobaccos by Matrix Attachment Regions

The matrix attachment region (MAR) located downstream of Plastocyanin gene was isolated from the genome of pea. To study the effect of MARs on foreign gene expression in transgenic plants, T-DNA vector was constructed in which MARs flanked bothβ-glucuronidase(GUS) gene and selectable marker neomycin phosphotransferase (NPT-II) gene. The plant expression vectors were transferred into leaf discs via Agrobacterium-mediated transformation procedure. The result of GUS measurement showed that pea MAR could increase transgene expression level. The mean expression levels of GUS gene expression in population containing MARs could be increased twofold when compared with that of population without MARs.     

金鱼养殖技术

本文给出金鱼养殖技术及措施,经济效益明显.     

血脂类代谢研究中的转基因动物模型

转基因疾病动物模型 ,通过不表达或过量表达的基因来表现各种疾病。脂质代谢是人体中的一种极为复杂的生理过程 ,在与脂质代谢有关的基因中 ,既有促进动脉粥样硬化形成的因素 ,也有降低动脉粥样硬化发病的成份。本文介绍了涉及脂质代谢有关的载脂蛋白 (Apo)类基因 ,如ApoE、ApoA、ApoB、ApoC ;相应的受体基因 ,如A型人清道夫受体SR A、SR B1和SR CD ;与脂质代谢有关的酶和转运蛋白的基因 ,如胆固醇脂转运蛋白 (CETP)、脂蛋白脂酶 (LPL)等几十种转基因动物模型及其特点 ,这些与血脂有关的转基因动物模型在研究发病机理、药物筛选…     

金鱼"突眼病"病原的初步研究

从患典型"突眼病"金鱼的眼球、肝脏中,分别分离出E-01和L-01菌,经人工感染试验确认E-01菌为该病的致病菌.该菌菌体大小为(1.0～1.5)μm×(1.5～2.3)μm,革兰氏阴性,无鞭毛;触酶阳性,氧化酶、硫化氢、吲哚、蔗糖、赖氨酸脱羧酶、鸟氨酸脱羧酶、精氨酸双水解酶等阴性.根据其生理生化特性确定为鲁氏不动杆菌(Acinebobacterlwoffi).药敏试验结果表明:其对先锋霉素V、卡那霉素、多粘菌素B、复方新诺明、庆大霉素、链霉素、新霉素等药物敏感.     

以新换旧--用独立通风笼盒(IVC)取代弹性薄膜隔离器

独立通风笼盒是应生物密封标准不断提高的需要而发明的。这些笼盒为动物提供了一个密封的环境 ,并且对空气携带的污染物的阻断也有很大的帮助。由于转基因动物增多 ,独立通风笼盒应用越来越广泛。它能有效地预防交叉污染 ,并且能可不同来源的动物住在一块 ,而不需要考虑它们的健康状况。本篇将对独立通风笼盒和弹性薄膜隔离器做一个比较。传统的弹性薄膜隔离器被用来把健康状况已经明确的动物放在一起 ,如 ,悉生动物和无菌动物。隔离器是二战时期特雷克斯勒和雷尼尔为研究恙虫病时发明的。如今 ,用隔离器来给动物提供住处经常会遇到这样的问…     

p21HBsAg/HBsAg和P21HBX/HBX转基因小鼠不同月龄血液生理指标的测定

目的检测p21^HBsAg/HBsAg和p21^HBX/HBX转基因小鼠的血液生理指标，分析p21^HBsAg/HBsAg和p21^HBX/HBX基因引入小鼠基因组p21位点中对其血液指标的影响。方法用日本光电MEK-5126K血球计数仪（动物芯片）及配套试剂盒对四个月龄不同基因型小鼠的血液生理指标进行测定，并且与野生型对照组进行比较。结果多数指标雌雄之间差异显著，并且两种基因型小鼠及野生型在不同年龄、不同指标的动态变化不同。结论p21^HBsAg/HBsAg和p21^HBX/HBX基因转入小鼠基因组p21位点对其血液生理指标具有一定的影响。     

Two different gene loci related to the spatial patterning of brain ventricle in vertebrate

Observations on living embryonic brains and the microstructure of brain ventricle of goldfish revealed that there are two brain ventricle phenotypes in gynogenetic haploid embryos. One phenotype is as normal as that of the control inbreeding diploid embryos, which has normal differentiated forebrain, midbrain and hindbrain. Another phenotype is obviously abnormal, the brain patterning is irregular, and no distinct brain ventricle can be observed. The ratio of haploid embryos with normal brain pattern to that with abnormal brain pattern is 1∶3. This ratio indicates that there are two gene loci involved in the spatial patterning of the brain ventricle. Since the possibility that deleterious recessive mutant alleles exist on both of the two gene loci had been excluded in this experiment, the phenotype represented the expressional state rather than the genotype of these two genes. Therefore, the ratio of 1∶3 suggests that the expressing probability for each copy of the two genes is 50%, and the regulatory mechanism of the expression is based on two sets of chromosomes, controlled by the rule of the diploid-dependent regulatory mechanism.     

转双价基因棉花的大田抗蚜性研究

文章利用昆虫生态学手段,以单价Bt棉和常规棉为对照,研究转Bt GNA双价基因棉的大田抗蚜效果。     

马铃薯转基因株系载体骨架的整合分析

对衍生于常用载体pBI 121的4种表达载体所获得的马铃薯转基因株系进行了PCR骨架整合分析,结果表明,左边界序列和短片段骨架基因更容易整合进基因组中,转入基因组的载体骨架片段的大小也不一样.这说明在转基因植株的检测中要注意骨架载体是否转入,以便消除生物安全性问题.     

转基因植物生物反应器的研究

综述了利用转基因植物作为生物反应器生产外源蛋白质,包括抗体、疫苗、药用蛋白等的优势及研究进展,并对转基因植物生物反应器生产蛋白类药物存在的问题及相关对策进行了论述。