稀土对银杏组培芽苗生长及其不定根的影响

以银杏成熟胚诱导的无菌苗茎段为外植体,探讨稀土[La(NO3)3]对其茎段顶芽高度、叶面积及芽苗不定根发生的影响。结果表明:稀土处理对芽高影响不显著但显著增加叶片的面积;而稀土对芽苗不定根的发生具有显著的促进作用。银杏的芽苗生根在附加1/2 MS 0.5 mg/L IBA的条件下,最高生根率仅33.3%(44号品种);而以1/2 MS 0.5 mg/L IBA为基本培养基附加0.5~5.0 mg/L的稀土则能显著促进不定根的发生,其中以1.0 mg/L稀土处理的79号品种及0.5 mg/L稀土处理的28号品种生根率最高,分别为66.7%,58.3%,效果极显著,而且生根时间比对照提前7d,根数也明显多于对照。高浓度的稀土(10~20 mg/L)则抑制银杏芽苗不定根的发生。     

百合子房的组织培养

以百合子房为材料进行组培快繁，得到子房诱导成愈伤组织的最适培养基为ＭＳ＋ＢＡ１ｍｇ／Ｌ＋ＮＡＡ０．５ｍｇ／Ｌ：适合诱导分化的培养基为ＭＳ＋ＢＡ０．５ｍｇ／Ｌ＋ＩＡＡ０．５ｍｇ／Ｌ；适合生根的培养基为ＭＳ＋ＢＡ０．２ｍｇ／Ｌ＋ＩＡＡ１ｍｇ／Ｌ．     

银线草无性系建立的研究

为满足栽培对种苗的需要,并对野生资源进行保护,本研究以银线草嫩茎为材料,采用组织培养的方法,进行了嫩茎愈伤组织的诱导与分化、试管苗的生根培养,以及移栽和定植的研究,建立起银线草嫩茎的无性系.研究结果表明:MS+ZT 0.2 mg/L+2,4-D 2.0 mg/L是愈伤组织诱导培养和继代培养的理想培养基;MS+BA0.6...     

Constructing Failure: Leonard Hayflick,Biomedicine, and the Problems with Tissue Culture

By examining the use of tissue culture in post-war American biomedicine, this paper investigates how scientists experience and manage failure. I study how Leonard Hayflick forged his new definition of failure and ways of managing it by refuting Alexis Carrel's definition of failure alongside his theory of the immortality of cultured cells. Unlike Carrel, Hayflick claimed that every vertebrate somatic cell should eventually die, unless it transformed into a tumour cell. This claim defined cell death, which had been a problem leading to a laboratory failure, as a normal phenomenon. On the other hand, permanent life, which had been considered a normal cellular characteristic, became a major factor causing scientific failure, since it implied malignant transformation that scientists hoped to control. Hayflick then asserted that his cell strains and method would partly enable scientists to manage this factor—especially that occurred through viral infection—alongside other causes of failure in routine tasks, including bacterial contamination. I argue that the growing biomedical enterprise fostered this work of Hayflick's, which had repercussions in both his career and the uses of cells in diverse investigations. His redefinition of failure in the age of biomedicine resulted in the broad dissemination of his cells, medium, and method as well as his long struggle with the National Institutes of Health (NIH), which caused his temporarily failed career.     

后黄卷叶蛾(Homona magnanima Diaknoff)质型多角体病毒的研究

<正>本文对后黄卷叶蛾质型多角体病毒(Hm cpv)进行了形态、超微结构,核酸性质,组织病理及毒力测定等方面的研究,该病毒的包含体呈六角形或园球形,大小为0.42～2.25μm。病毒粒子为正廾面体,表面具管状突起,大小为42～62nm。Hmcpv对后黄卷叶蛾5龄幼虫的LC_(50)为3.5×10~5PIB/ml,1×10~6PIB/ml的LT_(50)为10.4天。     

太白米愈伤组织的诱导

把太白米小鳞茎基部０．１－０．５ｍｍ处切口，接种于ＭＳ＋ＮＡＡ １ｍｇ／Ｌ＋２．４－Ｄ１ｍｌ／Ｌ＋ＺＴ０．１ｍｇ／Ｌ和ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ＋ＺＴ０．１ｍｇ／Ｌ培养基上，置黑暗中培养，小鳞茎能脱分化产生大量愈伤组织，诱导率在９５％以上，且生长迅速。     

番茄组织培养体系的建立及其影响因素的研究

以11种番茄（lylopersicon esculentum Mill)栽培品种为实验材料，以10－12天为无菌苗的子叶、下胚轴段、叶片、茎段为外植体，通过对供体基因型、激素浓度及培养条件的研究，建立了一套优化的适于番茄遗传转化的组织培养与再生体系。     

实施素质教育的思考

为适应知识经济时代人才竞争的需要，必须全面推进素质教育，本文在深入认识素质教育的内涵，强化素质教育观念的基础上，提出应该注重培养学生的创新意识和团队精神，同时，通过校园文化建设提高学生的综合素质．     

布氏轮藻肌动蛋白基因家族的全基因组鉴定及表达分析

为了解肌动蛋白actin在轮藻植物生长发育中的潜在功能,本研究利用生物信息学方法对布氏轮藻肌动蛋白基因家族进行了鉴定及表达分析.在布氏轮藻中共鉴定出16个肌动蛋白基因,将其重命名为CbACT1～CbACT16,其氨基酸长度为361～1182 AA,相对分子质量为39 886.71～117 256.72 Da,等电点介于4.68～8.93;亚细胞定位预测显示15个肌动蛋白基因位于细胞质中,1个位于叶绿体中;二级结构结果表明肌动蛋白基因主要以无规则卷曲和α螺旋为主;共线性分析中仅发现一对源自片段重复的旁系同源基因;系统发育结果表明轮藻肌动蛋白基因可以分为两个亚家族,结合基因结构及保守基序分析,同一亚家族倾向于具有相似的外显子分布,较多的共有基序;基于启动子顺势作用元件分析表明,16个肌动蛋白基因参与了许多与生物和非生物胁迫、激素调节和光反应有关的生命活动.组织表达分析显示,16个基因在四种不同组织中存在差异性表达,表明它们在不同组织的生长发育过程中具有不同的功能.因此,轮藻肌动蛋白基因家族可能参与了轮藻植物不同组织的发育过程及响应生物和非生物胁迫等的过程.     

组织培养在菊花辐射育种中的应用研究

组织培养应用于菊药辐射诱变育种，可以提高突变显现机率，增加选择机会，变异分离纯合速度快，育种周期短，效果好。