宁夏固原百里香挥发油化学成分的GC-MS研究

用水蒸气蒸馏法提取宁夏固原百里香挥发油，利用气相色谱-质谱联用法(GC-MS)对其化学成分进行了分析鉴定，共鉴定出31种组分．用归-化法对各成分进行定量，含量最高的4种组分为香荆芥酚(28．54％)，1-甲基-3-异丙基苯(22．77％)，百里香酚(11．45％)和1-甲基-4-异丙基-1，4-环己二烯(9．78％)，31种组分共占挥发油总量的98．33％．     

半导体激光(650 nm,5 mW)血管内照射对人外周血淋巴细胞凋亡的影响

报道了用半导体激光进行血管内照射（ＩＬＩＢ）治疗１３例患者外周血淋巴细胞凋亡的影响．发现照射前ＰＣＤ为（５．４７±３．６５）％，第一次照射后为（７．１±３．１６）％，第五次照射后为（１６．７１±９．５）％．两者均有非常显著性差异（Ｐ＜０．０１）．     

Evidence for suppression of immune function by insulin-like growth factor-1 in dwarf rats in vivo

These studies were undertaken to investigate the effects of increasing or decreasing IGF-1 levels on aspects of immune function in rats. Female dwarf rats were treated with recombinant human IGF-1 or with a potent sheep anti-IGF-serum. Body weight, thymus weight and spleen weight increased with IGF-1 treatment (p<0.001), while there was no effect of anti-IGF-1 treatment when compared with the appropriate normal sheep serum (NSS) treated controls. IGF-1 treatment significantly decreased WBC and RBC counts, but increased the ratio of CD4⁺:CD8⁺ T-cells. Anti-IGF-1 serum had no effect on these parameters compared with NSS. However anti-IGF-1 was associated with increased T-cell numbers, decreased natural killer cells, and enhancement of the animals' ability to produce specific IgG in response to injection of keyhole limpet haemocyanin (KLH). These results indicate that IGF-1 may suppress immune function although increasing the size of immune organs such as spleen.These studies were part of an M.Sc. at the University of Waikato, Hamilton, New Zealand.     

Phenotypic and Functional Analysis of Porcine T Lymphocytes

IntroductionPorcine is a useful model for the heterogeneity ofmammalian immune systems and has also receivedattention as a possible source of organs for humantransplants[1] .T lymphocytes are an importantcomponent of immune cells which directly inducecellular immune response and play a key role inimmune regulation.The cognition,activation,andfunction of T lymphocytes depend on interactionsbetween T cells and antigen presenting cells,aswell as B lymphocytes and other cells.The basesfor the in…     

Distribution of the 1,25 dihydroxy-vitamin D3 receptor in the bursa of Fabricius of chicken

The vitamin D₃ metabolite 1,25(OH)₂D₃ is probably involved in B lymphocyte ontogeny. We therefore determined the distribution of the 1,25(OH)₂D₃ receptor in the bursa of Fabricius and spleen cells of 7-day-old chicks, by immunohistochemistry using a monoclonal antibody against the chick intestinal cell 1,25(OH)₂D₃ receptor. The bursa cells of young (7-day-old) chicks contained large amounts of receptor while the spleen cells did not. The bursa cells of older (35-day-old) chicks contained fewer receptors, but the number of receptors in the spleen increased.     

瞬态电磁脉冲对淋巴细胞遗传及免疫功能的影响

通过以人的 T-淋巴细胞为受试对象 ,以其结合绵羊红细胞形成 E-玫瑰花结的百分率为指标 ,测试了不同波形参数的脉冲电磁场、不同照射时间对 E-玫瑰花结形成率的影响 ;并讨论了瞬态电磁脉冲(TEMP)对长白猪淋巴细胞转化率等遗传学效应的影响 ,为解释低强度瞬态电磁脉冲对细胞产生非热效应作用的机理提供了可靠的依据     

胸腺素对荷瘤小鼠胸腺淋巴细胞有丝分裂指数影响的研究

本文报道胸腺素对荷瘤小鼠胸腺淋巴细胞有丝分裂指数的影响。实验结果表明,注射胸腺素的实验组胸腺淋巴细咆有丝分裂指数均高于对照组,其中不同瘤龄之间有一定差异。     

褪黑激素与地塞米松对小鼠胸腺发育影响的生物化学与细胞生物学研究(Ⅰ)——褪黑激素对小鼠胸腺细胞大分子掺入能力及超氧化物歧化酶活性的影响

研究胸腺细胞体外培养条件下的~3H-Tdr、~3H-Ura及~3H-Phe的掺入能力,并对胸腺组织超氧化物歧化酶(SOD)的活性进行了测定。与对照组相比,经褪黑激素(Melatonin)处理的动物,其~3H-Tdr、~3H-Phe的掺入量分别增加57.1％与42.8％,~3H-Ura的掺入量变化不明显(1.10～1.48×10~)-2)dpm/cell);SOD活性增加了12.5％;同时发现,实验组动物的胸腺-体重比提高了14.1％。结果表明,褪黑激素对延缓小鼠胸腺的随龄退化有一定的效果。     

Growth hormone alters lymphocyte sub-populations and antibody production in dwarf rats in vivo

Female dwarf rats at different ages were treated with recombinant porcine GH or with a potent sheep anti-rat GH serum. Body weight and spleen weight increased with GH and decreased with anti-GH treatment (p<0.001). Neither GH nor anti-GH treatment resulted in a change in circulating WBCs, but GH decreased, while anti-GH increased, RBC counts (p<0.001). Similarly, GH treatment tended to decrease the ratio of CD4⁺:CD8⁺ T-cells while anti-GH increased (p<0.05) the ratio. Anti-GH treatment also enhanced the animals' ability to produce specific IgG in response to KLH injection. These results indicate that GH may have a physiological role in suppressing humoral immune function but may enhance cell-mediated immunity.     

Perforin and its role in T lymphocyte-mediated cytolysis

The killing mediated by cytotoxic T lymphocytes (CTL) represents an important mechanism in the immune defence against tumors and virus infections. The lytic mechanism has been proposed to consist of a polarized secretion of granule-stored molecules, occurring on effector-target cell contact. By electron microscopy, membrane deposited, pore-like lesions are detected on the target cell membrane during cytolysis by CTL. These structures resembled strikingly pores formed during complement attack.Granules of CTL isolated by nitrogen cavitation and Percoll gradient centrifugation were shown to retain cytotoxic activity. Further purification of proteins stored in these granules led to the discovery of a membranolytic protein named perforin which was capable of polymerizing into pore-like structures. In addition to this cytolytic protein, a set of serine esterases was found as well as lysosomal enzymes and proteoglycans, whose function are not yet clearly defined. The role of perforin in the cytotoxic process is currently being explored by ablating the active gene in mice.