论晚清步调不一的诗界革命和小说界革命——以梁启超、黄遵宪为中心

评价梁启超、黄遵宪等人的诗界革命,可以联系他们对小说界革命的看法,他们面对两种文类革新时发言的姿态是很不一样的。在以激进的言论探讨小说界革命的同时,对诗界革命的态度却是审慎的,甚至趋向保守的。这说明不同的文类成规对美学变革有不同的制约作用。梳理和比较梁启超、黄遵宪等人面对诗界革命和小说界革命的不同反应,可以借此更深入地反思古典成规对诗歌革新的强大牵引力。     

中国男子篮球可持续发展探析

通过对第五届城市运动会男子篮球赛共计40场比赛统计数据进行分析处理，探讨我国男篮可持续发展情况。     

浅谈如何提高独立学院图书馆的期刊利用率

介绍了独立学院图书馆的发展现状,分析了影响期刊利用率的主要因素,提出了提高期刊利用率的有效途径。     

MGB probe assay for rapid detection of mtDNAl1778 mutation in the Chinese LHON patients by real-time PCR

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.     

Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis

A new feather-degrading bacterium was isolated from a local feather waste site and identified as Bacillus subtilis based on morphological, physiochemical, and phylogenetic characteristics. Screening for mutants with elevated keratinolytic activity using N-methyl-N＇-nitro-N-nitrosoguanidine mutagenesis resulted in a mutant strain KD-N2 producing keratinolytic activity about 2.5 times that of the wild-type strain. The mutant strain produced inducible keratinase in different substrates of feathers, hair, wool and silk under submerged cultivation. Scanning electron microscopy studies showed the degradation of feathers, hair and silk by the keratinase. The optimal conditions for keratinase production include initial pH of 7.5, inoculum size of 2% （v/v）, age of inoculum of 16 h, and cultivation at 23 ℃. The maximum keratinolytic activity of KD-N2 was achieved after 30 h. Essential amino acids like threonine, valine, methionine as well as ammonia were produced when feathers were used as substrates. Strain KD-N2, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production.     

云南壮剧的表演艺术形态

云南壮剧既借鉴了广戏等周边剧种的表演艺术，又吸收、融化了本民族的武术、舞蹈动作，还提炼、吸收了大量生活动作。在角色行当划分方面，云南壮剧经历了一个从无到有、由简到繁、繁简并存的历程，到解放前夕，富宁土戏、广南东路沙戏的角色行当划分比较细致，而广南北路沙戏、文山乐西土戏的角色行当划分则比较简略。     

大学英语教师的自主性分析

自主性问题一直是外语教学的热门话题，教师自主和学生自主能否实现是关系到我国大学英语教学改革成功与否的关键因素之一。本文通过分析当前大学英语教育的现状，指出学生自主很大程度上取决于教师自主，教师在英语教学中扮演着传授者、组织者、启发者、合作者的角色。     

论张志和《渔歌子》的江南话语

江南,是张志和《渔歌子》建构的核心意象。通过对历史语境的还原和文本内涵的解读,不难发现这一意象,不仅是张志葙的叙述场域,也是一种叙述话语,即江南话语,它传达出了隐逸诗人张志和在“家国同构”的认同传统下内心激烈的斗争和挣扎。     

义务教育教师专业化发展评价指标体系的构建——以合肥市中小学教师为例

采用文献法、调查法,在对合肥市义务教育阶段中小学教师专业化发展调查分析的基础上,尝试构建了义务教育教师专业化发展评价指标体系,期望为不断提高义务教育教师的专业化发展水平提供新的思路。     

地下水在渐变各向异性介质中的渗流速度方程的改进

Darcy定律是地下水动力学的基础公式,也是描述地下水运动状态的基本公式。怎样应用Darcy定律全面而简单的表达地下水在渐变各向异性介质中的流速方程是本文的目的。