Evaluation of the hepatotoxicological effects of a drug in an in vivo/in vitro model

Both in vivo and in vitro models have certain disadvantages for the study of the chronic hepatotoxicity of drugs. The aim of this work was to evaluate a new approach based on an in vivo/in vitro model. After chronic in vivo treatment of rats with Vincamine and Vindeburnol (an eburnamenine derivative which exhibits hepatotoxic properties in man) liver cells were isolated, and functional and metabolic disorders (metabolic utilization of fructose and protein biosynthesis) were studied to determine injury. The results showed no modification of blood parameters, but a direct relationship between the dose of Vindeburnol administered in vivo and the metabolic disorders observed in vitro, evidencing the high sensitivity and reliability of this model.     

实现净产值指标刍议

本文主要阐述了生产净产值指标在评价企业经济效益时的弊病,并从理论上和实践上论述了在商品经济条件下采用实现净产值指标评价企业经济效益的重要性和必要性。同时介绍了实现净产值指标的两种计算方法。即:生产法、分配法。     

Suitability of urethane anesthesia for physiopharmacological investigations in various systems Part 1: General considerations

Summary The suitability of urethane anesthesia for physiopharmacological investigations is reviewed. Total dose administered and route of administration are recognized as factors having a great influence on both resting parameters and biological responses to drugs. A peculiar characteristic of urethane is represented by its ability to induce a surgical plane of anesthesia without affecting neurotransmission in various subcortical areas and the peripheral nervous system. This makes urethane a suitable general anesthetic for studying neural function in both central and peripheral nervous systems and accounts for the preservation of a number of reflex responses in urethane-anesthetized animals.     

The in vivo expression of the globin genes of theβ cistron in γ-,δ-, andδβ-thalassemia heterozygotes

There is considerable evidence suggesting that the switch from to and chain production after birth is due, in part, to silencing of the genes by stage-specific factors which bind to their promoters and to the competition from the adult ( and ) genes for a common enhancer element located in the locus control region. As a consequence one can expect that the increased Hb F production in adults with hereditary persistence of fetal hemoglobin or -thalassemia is directed mainly by -globin genes in cis to the deletion(s) responsible for these conditions. Here we review data on heterozygotes with -, -, or -thalassemia, who also had an^AT mutation, in cis or in trans, which was used as a marker of gene expression. The results show that a deletion affecting adult genes favors the expression of genes in cis, while the deletion of a single gene does not affect the expression of the gene in cis but leads to a faster switch postnatally.     

Detection of toxic metabolites in the hemolymph ofBeauveria bassiana infectedSpodoptera exigua larvae

Highly active metabolites have been detected in the hemolymph of the lepidopteranSpodoptera exigua infected with the mycopathogen,Beauveria bassiana. A combination of phenyl sepharose and CM ion exchange chromatography was utilized to extract the active metabolites from infected hemolymph samples. The active in vivo metabolites, having a molecular mass greater than 10 KDa, were thermolabile and were inactivated by proteinase K. These metabolites were characterized by their ability to disrupt metamorphosis, killing treated larvae at the wandering or pupal stage. Additionally, injection ofS. exigua larvae with active samples caused a reduction in the number of filopodial-producing hemocytes. The biological activities and biochemical properties suggest that novel compounds are produced duringB. bassiana mycosis.     

车前的离体培养和植株再生

报道了国丧外植体的愈伤组织诱导及其植株再生的研究结果,单独的外源生长素２,４－Ｄ适宜诱导外植体脱分化产生愈伤组织。再生芽的分化只需添加６－ＢＡ;当ＮＡＡ与６－ＢＡ配合添加于培养基中时,则有利于再生根的繁茂生长,从而形成完整的再生植株。     

批量制造业模糊交货期准时化生产计划方法

通过建立批量制造业模糊交货期下的准时化生产计划模型,将隶属度函数引入到生产计划问题中,从而拓宽了准时化生产计划问题的研究范围,并为实际生产计划的编制提供了一种有效途径。     

高温氧化膜Cr2O3和NiO中应力松弛过程

用Ｘ射线原位应力测定了高温氧化膜Ｃｒ２Ｏ３和ＮｉＯ中的应力．结果表明,Ｃｒ２Ｏ３／Ｃｒ和ＮｉＯ／Ｎｉ体系氧化膜的生长应力分别为－１．９４９ＧＰａ和６５２ＭＰａ．在冷却过程中,Ｃｒ２Ｏ３／Ｃｒ体系发生很大的应力松弛过程,其主要表现形式为氧化膜翘曲和开裂;而在ＮｉＯ／Ｎｉ体系中,由于ＮｉＯ薄膜具有双层结构承受不同应力状态而获得松弛．     

核酶的化学合成及其对烟草花叶病毒RNA片段的体外剪切

设计并用化学方法合成了针对烟草花叶病毒ＲＮＡ的核酶ＲＺ－１及ＲＺ－１小型化的核酶ＲＺ－２以及它们的底物ＲＮＡ片段Ｓｂ,研究了两种核酶对底物Ｓｂ的体外催化活性,并把核酶Ｒｚ－１与生物合成的相同组成的核酶Ｒｚ－１’进行了比较试验。     

科技进步统计监测软件系统的设计与实现

河北省科技进步统计监测软件系统以科技进步统计监测评价需求为指导,进行了合理的总体结构设计、系统功能设计、数据库设计、输入输出设计、存储设计,并在河北省大中型工业企业的科技进步统计监测评价中得到了应用。