居住区交通噪声污染影响分析及防治对策的研究——以大连市泉水居住区为例

采用德国Datakustik公司出品的Cadna/A软件,模拟预测了大连市泉水居住区开发建设后交通噪声污染的影响,提出了切实可行的交通噪声防治对策,对其它居住区开发建设具有借鉴意义.     

亮菌研究的现状与展望

亮菌属小蜜环菌属,是一种药食兼用的名贵真菌.从亮菌名称的由来及分类、亮菌的生物学特性、亮菌中所含的亮菌甲素和亮菌多糖等化学物质、亮菌的固态和液体发酵培养、亮菌的抗炎、防辐射、升白、抗肿瘤、增强免疫力等生物学功能5个方面对亮菌的研究现状进行了详细的综述,并对亮菌的研究前景进行了展望,为亮菌的进一步开发及研究提供参考.     

3种芦荟多糖体外抗肿瘤作用的比较研究

比较研究了3种芦荟多糖的体外抗肿瘤药效.采用体外培养法培养细胞,MTT比色法观察3种芦荟多糖在7个质量浓度下对Hela、K562和A973细胞株的体外直接作用.结果,随着芦荟多糖质量浓度的增加,对3种肿瘤细胞的抑制作用不断增强.经计算,对于Hela、K562和A9733种人体肿瘤细胞,库拉索芦荟多糖的IC50分别为183 40、170 26和151 12Qg/mL;木立芦荟多糖的IC50分别为191 18、125 44和49 01欤?mL;中国芦荟多糖的IC50分别为78 79、138 02和114 48欤?mL,均高于10嚕?mL.倒置显微镜下观察发现,除200、400嚕?mL两剂量外,给药后各孔细胞形态良好,与空白对照组比较无明显变化.以上结果提示3种芦荟多糖对上述3种瘤细胞直接作用较弱.     

基于CS5460A的三相智能电能表的设计

介绍了一种基于CS5460A 的三相智能电能表的设计.该电能表有精确计时、分时管理、电能统计等功能,可以对电能表进行数据读取、校准等操作.其硬件部分主要采用电能表专用芯片CS5460A及单片机AT89S51等,软件部分采用C语言编写.给出了部分硬件电路图,讨论了软件模块的设计方法.对设计中需要注意的问题进行了分析.     

简化磺胺乙酰化测定CoA

本文报道测定辅酶A(CoA)活力的简化磺胺乙酰化法。该法用鲜鸽肝酶液代替鸽肝丙酮粉,设计了一简单装置贮存鲜酶液,简化了操作程序,测定准确,重现性好。     

盐度对秋茄和桐花树幼苗蛋白质、H2O2及脂质过氧化作用的影响

研究两种红树植物秋茄和桐花树幼苗根和叶在不同盐度培养下，蛋白质、丙二醛及过氧化氢含量的变化情况，结果表明：盐度对秋茄和桐花树幼根和幼叶蛋白质含量都有,总体上是低盐度稍降低其含量，高盐度稍增加其含量。秋茄幼根和幼叶在0－50盐度范围内MDA含量随盐度的升高先降后升，但是根和叶上升的折点不同（根30，叶20），而桐花树幼根MDA含量在盐度30范围内比较平稳，盐度高于30时则迅速上升，而幼叶在盐度20以内下降，高于20则上升。秋茄和桐花树的幼根在低盐情况下H2O2含量随盐度的增加而下降，而幼叶都表现出先降后升再降的趋势，只是上升的折点不同而已（秋茄40，桐花树20）。这些结果表明，盐度对秋茄和桐花树幼根和幼叶的H2O2含量及膜脂过氧化作用是有一定差异的。     

微波作用下NaA沸石与C~(2 )a的交换反应研究

研究了微波作用下，ＮａＡ沸石中的Ｎａ＋被Ｃａ２＋交换的反应．反应可在１０ｍｉｎ内完成，而在常规加热下需１ｈ．考察了交换时间、交换液浓度及容器底面积对交换度的影响；用ＸＲＤ、ＤＴＡ、ＳＥＭ表征了样品，并对结果进行了讨论     

拟南芥逆境胁迫诱导转录因子DREB1A基因的克隆和序列分析

以拟南芥基因组DNA为模板,通过PCR扩增得到逆境胁迫诱导转录因子DREB1A基因,将其克隆到pUC19质粒中.序列分析表明获得的基因序列与已报道的该基因的序列完全相同.     

Developing pharmacological therapies for Alzheimer disease

Alzheimer disease (AD), while chronic and progressive with an average progression of 7 – 10 years, is both multifactorial and heterogeneous. Thus, AD offers a large window of opportunity and a large number of therapeutic targets to inhibit it. The selection of a therapeutic target, however, is one of the biggest challenges in developing a pharmacological treatment of this multifactorial disease. Inhibition of a pivotal downstream event is likely to benefit more patients than inhibition of an upstream event in AD pathogenesis. Neurofibrillary degeneration of abnormally hyperphosphorylated tau offers such a pivotal therapeutic target. Abnormal hyperphosphorylation of tau and not its aggregation into filaments appears to be the most deleterious step in neurofibrillary degeneration. Tau can be abnormally hyperphosphorylated by downregulation of protein phosphatase-2A activity or by upregulation of more than one tau kinase. Restoration of the phosphatase activity which is downregulated in AD brain or inhibition of GSK-3β and cdk5, which are required for AD-type abnormal hyperphosphorylation of tau, are among the most promising therapeutic strategies.     

Regulation of cell adhesion by PP2A and SV40 small tumor antigen: An important link to cell transformation

The serine/threonine protein phosphatase 2A (PP2A) represents a large family of highly conserved heterotrimeric enzymes. Their critical importance in cell homeostasis is underlined by the fact that they are targets of natural toxins like the tumor promoter okadaic acid, and of simian virus 40 small tumor antigen (SV40 small t), a viral protein known to promote cell transformation. Furthermore, mutated or lower expression levels of PP2A subunits have been found in certain cancers. One major known event in PP2A-dependent cell transformation is the alteration of key signaling pathways that control cell growth and survival. In this review, we focus on how PP2A enzymes also affect cell adhesion and cytoskeletal dynamics, the disruption of which is linked to loss of cell polarity, increased cell motility and invasiveness. We also examine how those various pathways participate in the transforming activity of SV40 small t. Received 29 June 2006; received after revision 3 August 2006; accepted 20 September 2006