Species-specific differences in the mitogenic activity of heparin-binding growth factors in the sera of various mammals

Sera from different mammalian species displayed great differences in mitogenic activity, as measured by stimulation of DNA synthesis in BALB/c 3T3 cells (3T3 cells). Among the sera examined, fetal bovine serum was least active, and increasing activity was detected in calf serum, human serum, rat serum and mouse serum, in that order. Rat and mouse sera exhibited extremely high mitogenic activity with 3T3 cells, but when TIG-1 human fetal lung fibroblasts were used for the DNA assay instead, the activity levels of all of the sera were lower, and the differences between them were smaller. To determine the reasons for these differences, the heparin-binding growth factors in each serum were separated on a heparin affinity column. Five peaks of DNA-stimulating activity were obtained. Three of these were found in all sera examined, with both 3T3 cells and TIG-1 cells. Two other peaks were found only with 3T3 cells; one was peculiar to rat and mouse sera, with extremely high activity in the rat, and the other was specific to fetal serum. The dependence of the activity of these peaks on the cells used for the test was confirmed using normal rat lung fibroblasts and immortalized rat kidney cells. These findings adequately explain the species-specific differences in mitogenic activity of whole sera, and the variation in activity depending on the cells used for assay of DNA synthesis.     

小鼠脾脏和SRS淋巴瘤体外培养细胞株的电泳行为

本文比较了SRS淋巴瘤体外培养细胞株及其宿主小鼠脾脏细胞的电泳行为。两种细胞都具有快、慢两个电泳主峰。经抗θ血清处理和尼龙柱过滤的结果表明,快峰为T细胞,慢峰为B细胞。SRS淋巴瘤细胞体外培养株,主要是T,B混合细胞型,其中B细胞约占60%～70%,T细胞约占30%～40%。     

普通小麦T型与V型不育系花粉发育过程中苹果酸脱氢酶的变异

对不同发育时期花药的苹果酸脱氢酶(MDH)同工酶的研究表明:线粒体MDH(m-MDH)同工酶谱型是保守的,即只有量上的变化,质的差异不明显;不同类型不育系植株的胞浆MDH(s-MDH)同工酶表现不同:T型植株在花粉发育的整个时期s-MDH都存在,而V型植株在花粉发育的二胞期才出现s-MDH;此两不育类型植株从未出现微体MDH(g-MDH)。     

Human peripheral blood monuclear cells transfected with messenger RNA stimulate antigen-specific cytotoxic T-lymphocytes in vitro

The efficiency of test vaccines needs to be evaluated by quantification of the triggered cellular immune response. Usually, for these assays, autologous target cells expressing the vaccine antigen are required. In the context of messenger RNA (mRNA)-based vaccinations, the target cells used for the read-out are mRNA-transfected monocyte-derived dendritic cells (Mo-DCs). Their production typically requires samples of 100 ml blood from the patients, and limits the number of assays that can be performed. We show here that fresh peripheral blood mononuclear cells (PBMCs) can be transfected with mRNA by electroporation. Such cells are as efficient as mRNA-transfected Mo-DCs for their ability to activate memory T cells in vitro. Thus, mRNA-transfected PBMCs are a convenient replacement of mRNA-transfected Mo-DCs for the in vitro monitoring of natural or vaccine-induced immune responses.Received 17 February 2005; received after revision 1 May 2005; accepted 7 Juni 2005     

关于分离性的一点注记

本文引入CS分离性,讨论了它与T_0、T、T_1和正则空间的关系,最后给出CS空间几个重要性质.     

快速消解加热法测定水中高锰酸盐指数

用HCA-10O标准消解器进行快速加热,控制加热时间,并设有风冷加水冷的冷凝回流,同时减小加药体积,快速消解加热测定了地表水的高锰酸盐指数,该方法缩短了实验时间,短时快速测定出高锰酸盐指数,确定了稀释倍数,避免了多次反复稀释,提高了工作效率,适应了日益繁重的水质监测工作要求.     

多曲面槽式聚光太阳电池电热联供系统性能研究

介绍了一种多曲面槽式太阳能聚光器的工作原理,对该装置进行了三维建模,利用光学分析软件对该聚光器安装平板式太阳电池进行光线追迹分析,直观地再现了聚焦光线的分布.基于该多曲面槽式聚光系统,提出一种新型的聚光太阳电池电热联供系统(TCPV/T).该系统能够有效利用太阳辐射能量,提高太阳电池输出电功率、光电转换效率,并将太阳电池产生的热量有效回收,实现聚光发电系统对外输出电能、热能.构建了多曲面槽式聚光多晶硅太阳电池电热联供实验系统.实验结果表明,在约3倍太阳聚光作用下,与非聚光平板电池、安装于同一聚光器内的太阳电池输出电功率相比,聚光电热联供系统输出最大电功率分别提高了96.4%和64.2%,系统综合性能效率达到62.8%.     

周期性压力对T型微管内二元流体混合效果的影响

在微流动领域经常涉及到试剂的混合问题,但是在微设备中,雷诺数Re低,流体趋向于形成层流,很难发生有效的混合.通过对微管内流体施加周期性压力使流速随时间呈周期性变化可以有效促进微流动混合.运用格子Boltzmann方法模拟了T型微管内流体的流速随时间呈正弦曲线变化而导致的混沌现象.结果发现,混沌混合的效果取决于两流体流速的相位差、流速的变化周期和系统的雷诺数Re.     

冲击荷载作用下T型管节点变形初步分析

对两端简支的T型管节点支管受到圆柱形锤体冲击后的动力响应进行了数值分析,提出区分主管变形中局部凹陷变形和整体弯曲变形的方法,得到了冲击过程中2种变形的时程曲线以及与冲击力时程曲线变化的联系,并且得到了2种变形与冲击动能以及节点主管长径比之间的关系．数值分析结果表明：随着节点变形消耗的能量的增加,局部变形趋于稳定,变形的增长逐渐集中在整体变形上．