全文获取类型
收费全文 | 1196篇 |
免费 | 44篇 |
国内免费 | 71篇 |
专业分类
系统科学 | 57篇 |
丛书文集 | 42篇 |
教育与普及 | 8篇 |
理论与方法论 | 14篇 |
现状及发展 | 78篇 |
综合类 | 1111篇 |
自然研究 | 1篇 |
出版年
2024年 | 1篇 |
2023年 | 13篇 |
2022年 | 13篇 |
2021年 | 11篇 |
2020年 | 20篇 |
2019年 | 17篇 |
2018年 | 18篇 |
2017年 | 17篇 |
2016年 | 18篇 |
2015年 | 37篇 |
2014年 | 61篇 |
2013年 | 40篇 |
2012年 | 92篇 |
2011年 | 66篇 |
2010年 | 52篇 |
2009年 | 75篇 |
2008年 | 62篇 |
2007年 | 66篇 |
2006年 | 56篇 |
2005年 | 57篇 |
2004年 | 50篇 |
2003年 | 64篇 |
2002年 | 54篇 |
2001年 | 56篇 |
2000年 | 45篇 |
1999年 | 52篇 |
1998年 | 28篇 |
1997年 | 32篇 |
1996年 | 14篇 |
1995年 | 13篇 |
1994年 | 18篇 |
1993年 | 14篇 |
1992年 | 15篇 |
1991年 | 14篇 |
1990年 | 9篇 |
1989年 | 19篇 |
1988年 | 9篇 |
1987年 | 2篇 |
1986年 | 7篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有1311条查询结果,搜索用时 549 毫秒
61.
申世昌 《西南师范大学学报(自然科学版)》2002,27(5):696-699
研究了完美T形树T(l1,l2,l3)的匹配唯一性,给出了其匹配唯一的充分必要条件,定理A 设G=T(l1,l2,l3)是T形树,若l1,l2,l3至少有一对相等,则G必匹配等价于一类Q∪P型图。定理B 设G=T(l1,l2,l3)是完美T形树,则图G匹配唯一的充分必要条件是l1,l2,l3互不相等。 相似文献
62.
对CO和H2在483K、3.5MPa条件下,在充满F-T合成产物(平均相对分子质量400)或超临界正戊烷的催化剂微孔内的扩散速率、孔内气体浓度分布以及催化剂的内表面利用率进行了模拟计算。结果表明合成气在F-T产物中的扩散速率比超临界介质中低得多,从而造成了气相反应中从催化剂孔中到孔内显著的合成气浓度梯度及催化剂内表面利用率的下降,从理论上解释了超临界条件下CO转化率和烃收率优于气相反应的原因。 相似文献
64.
林柏钢 《福州大学学报(自然科学版)》1993,(4):42-48
应用布尔序集关系理论,研究了一类典型T BOSn 唯一路径簇的构造与实现方法.同时给出基本图形特性的分析. 相似文献
65.
Immune responses to DNA vaccines 总被引:16,自引:0,他引:16
DNA vaccines, based on plasmid vectors expressing an antigen under the control of a strong promoter, have been shown to induce
protective immune responses to a number of pathogens, including viruses, bacteria and parasites. They have also displayed
efficacy in treatment or prevention of cancer, allergic diseases and autoimmunity. Immunologically, DNA vaccines induce a
full spectrum of immune responses that include cytolytic T cells, T helper cells and antibodies. The immune response to DNA
vaccines can be enhanced by genetic engineering of the antigen to facilitate its presentation to B and T cells. Furthermore,
the immune response can be modulated by genetic adjuvants in the form of vectors expressing biologically active determinants
or by more traditional adjuvants that facilitate uptake of DNA into cells. The ease of genetic manipulation of DNA vaccines
invites their use not only as vaccines but also as research tools for immunologists and microbiologists.
Received 26 October 1998; received after revision 3 December 1998; accepted 3 December 1998 相似文献
66.
T cell activation is enhanced by the costimulatory interaction of B7 on antigen-presenting cells and CD28 on T cells, resulting
in long-term T cell proliferation, differentiation and production of large amounts of cytokines, such as interleukin (IL)-2.
CTLA-4 is a co-stimulation receptor that shares 31% homology with CD28 and binds B7 family members with higher affinity. CTLA-4
is transiently expressed intracellularly and on the cell surface following activation of T cells. We have studied the kinetics
of CTLA-4 expression and the effects of dexamethasone on CTLA-4 expression during T cell activation in cultures of mouse spleen
cells stimulated by a mixture of immobilized anti-CD3 and anti-CD28 monoclonal antibodies (anti-CD3/CD28 mAb) or concanavalin
A (ConA). CTLA-4 expression peaked on day 2 and returned to background levels after 7 days. Dexamethasone was found to potentiate
CTLA-4 expression in a dose-dependent manner with an EC50 effective concentration 50%) of about 10−8 M. In contrast, other immunosuppressive agents, such as rapamycin or cyclosporin A had no or an inhibitory effect on CTLA-4
expression, respectively. Dexamethasone also stimulated CD28 expression, but inhibited IL-2R expression during anti-CD3/CD28
mAb-induced mouse splenic T cell activation. Western blot analyses of lysates of activated mouse T cells showed that dexamethasone
increased CTLA-4 protein levels twofold during anti-CD3/CD28 mAb-induced activation. Dexamethasone also enhanced CTLA-4 messenger
RNA twofold as quantified by ribonuclease protection assay. The effects of dexamethasone on CTLA-4 expression were glucocorticoid-specific
and completely inhibited by the glucocorticoid receptor antagonist mifepristone (RU486), indicating that the effect of dexamethasone
on CTLA-4 expression is mediated through the glucocorticoid receptor. In conclusion, the immunosuppressive agent dexamethasone
actually stimulates CTLA-4 expression, which is involved in downregulation of T cell activation.
Received 19 May 1999; received after revision 13 July 1999; accepted 13 July 1999 相似文献
67.
论述了综合业务数字网(ISDN)及其标准用户──网络接口的基本概念;对用户网络接口的体系结构作了较为详细的说明,给出了具体的软件实现方法和硬件接口电路(SNIC).在此基础上,提出了ISDN标准数字话音终端和基于基本接入能力(2B+D)的多功能数字终端的设计方案。 相似文献
68.
Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines 总被引:3,自引:0,他引:3
Wang P Mariman E Keijer J Bouwman F Noben JP Robben J Renes J 《Cellular and molecular life sciences : CMLS》2004,61(18):2405-2417
Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of
adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to
profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation
was screened, and 41 proteins that responded to blocking of secretion by 20°C treatment and/or brefeldin A treatment
were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as
secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25
are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an
actively remodeling extracellular matrix and several factors involved in growth regulation.Received 15 June 2004; received after revision 26 July 2004; accepted 2 August 2004 相似文献
69.
Joyce S 《Cellular and molecular life sciences : CMLS》2001,58(3):442-469
Cellular and humoral immune mechanisms recruited to defend the host from infectious agents depend upon the early immune events
triggered by antigen. The cytokine milieu within which the immune response matures is the most important of many factors that
govern the nature of the immune response. Natural T cells, whose function is controlled by CD1d molecules, are an early source
of cytokines that can bestow type 1 or type 2 differentiative potential upon helper T lymphocytes. This review attempts to
illuminate the glycolipid antigen presentation properties of CD1d, how CD1d controls the function of natural T cells and how
CD1d and natural T cells interact to jump start the immune system. CD1d is postulated to function as a sensor, sensing alterations
in cellular lipid content by virtue of its affinity for such ligands. The presentation of a neo-self glycolipid, presumably
by infectious assault of antigen-presenting cells, activates natural T cells, which promptly release pro-inflammatory and
anti-inflammatory cytokines and jumpstart the immune system.
Received 10 July 2000; received after revision 16 October 2000, accepted 16 November 2000 相似文献
70.
Leiman PG Kanamaru S Mesyanzhinov VV Arisaka F Rossmann MG 《Cellular and molecular life sciences : CMLS》2003,60(11):2356-2370
Bacteriophage T4 is one of the most complex viruses. More than 40 different proteins form the mature virion, which consists of a protein shell encapsidating a 172-kbp double-stranded genomic DNA, a tail, and fibers, attached to the distal end of the tail. The fibers and the tail carry the host cell recognition sensors and are required for attachment of the phage to the cell surface. The tail also serves as a channel for delivery of the phage DNA from the head into the host cell cytoplasm. The tail is attached to the unique portal vertex of the head through which the phage DNA is packaged during head assembly. Similar to other phages, and also herpes viruses, the unique vertex is occupied by a dodecameric portal protein, which is involved in DNA packaging.Received 18 February 2003; received after revision 16 April 2003; accepted 9 May 2003 相似文献