SlNPV多角体蛋白基因的序列分析

ＳｌＮＰＶ多角体蛋白基因的开放读码框为７５０ｂｐ,编码２４９个氨基酸的蛋白质,是目前所发现的最长的多角体蛋白基因．ＳｌＮＰＶ多角体蛋白Ｍｒ＝２９２３６,其氨基酸序列与其它核多角体病毒的多角体蛋白氨基酸序列有高度同源性．     

单头饲养斜纹夜蛾增殖S1NPV病毒的研究

使用人工半合成饲料大规模单头饲养斜纹夜蛾幼虫，增殖核型多角体病毒，通过不同接种浓度、接种龄期、饲养温度以及不同饲料的比较，遴选出当３龄幼虫接种的病毒浓度为３．８５×１０＾５ＰＩＢｓ／ｍＬ，４龄幼虫接种的病毒浓度为３．８５×１０＾７ＰＩＢｓ／ｍＬ时，病毒产量最高，４龄幼虫接种增殖病毒的最适温度为２４－２７℃，人工半合成饲料饲养幼虫增殖病毒效果优于天然饲料，同时，罹病虫尸热处理瑟其病毒产量之间，有显     

γ和e在斜纹夜蛾病毒中诱发的ESR谱

测量和研究了γ射线和电子束在斜纹夜蛾核型多角体病毒(NPV)中诱发的长寿命自由基的ESR谱。长寿命自由基的浓度与辐射剂量呈线性关系,而与NPV存活率的关系为“肩型”曲线。辐射在NPV中诱发的自由基的ESR谱明显不同于热能诱发的自由基的ESR谱,并且ESR谱具有超精细结构。本文还提出了DNA断链的一种可能方式。     

甜菜夜蛾田间种群抗药性及其解毒代谢酶活性变化

用点滴法和浸叶法分别测定了同一地区不同年份甜菜夜蛾田间种群及室内饲养1年后的抗性种群3龄幼虫的抗药性水平。结果表明甜菜夜蛾田间种群对杀虫剂抗性水平的变化与解毒代谢酶活性密切相关。     

Involvement of Jasmonate-signaling pathway in the herbivore-induced rice plant defense

The expression patterns of eight defense- related genes in the herbivore-infested and jasmonate- treated (jasmonic acid, JA and its derivative MeJA) rice leaves were analyzed using RT-PCR. The results showed that Spodoptera litura Fabricius (Lepidoptera: Noctuidae) herbi-vory induced the expression of lipoxygenase (LOX) and al-lene oxide synthase (AOS) genes that are involved in the jasmonate-signaling pathway. Moreover, S. litura damage resulted in the expression of farnesyl pyrophosphate syn-thase (FPS), Bowman-birk proteinase inhibitor (BBPI), phenylalanine ammonia-lyase (PAL) and other rice defense- related genes that were also induced by aqueous JA treat-ment or gaseous MeJA treatment. These indicated that in rice leaves, the JA-related signaling pathway was involved in the S. litura-induced chemical defense. Mechanical damage and brown planthopper (BPH), Nilaparvata lugens (St錶) (Homoptera: Delphacidae) damage induced the expression of LOX gene, but both treatments did not induce the expression of AOS gene. However, BPH damage induced the expression of acidic pathogen-related protein 1 (PR-1a), Chitinase (PR-3), and PAL genes, which is involved in the salicylate- signaling pathway. It was suggested that salicylate-related signaling pathway or other pathways, rather than jas-monate-signaling pathway was involved in the BPH-induced rice plant defense.     

斜纹夜蛾几丁质酶家族基因鉴定与时空表达分析

昆虫GH18家族几丁质酶属于多基因家族,在昆虫的蜕皮与变态过程中发挥着至关重要的作用,它催化表皮几丁质的降解.斜纹夜蛾是一种重要的农业害虫,危害290多种植物,但对其几丁质酶未深入研究.该文通过斜纹夜蛾转录组学分析,发现了12种潜在的几丁质酶,分属于8类.对这些几丁质酶在斜纹夜蛾变态发育时在表皮、中肠以及脂肪体的时空表达分析结果表明,Sl Cht5,Sl Cht9和Sl Cht2的表达水平在幼虫转化为蛹时上调表达,说明这些几丁质酶可能参与了斜纹夜蛾变态时期的脱皮作用;而其他几丁质酶的表达具有组织特性,表明它们可能具有不同功能,如Sl Cht6仅在中肠和脂肪体中表达,Sl Cht11,Sl Cht12和Sl Cht16主要在中肠和表皮表达,Sl IDGF2仅在表皮有表达,Sl IDGF3和Sl Cht9在三大组织均有表达.本研究结果对斜纹夜蛾几丁质酶家族基因的鉴定和时空表达作了初步的分析,为研究它们在斜纹夜蛾发育中的功能与调控打下基础.