Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV

Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV.     

“三环制“供热系统中热水一级网运行计算研究

针对"三环制"供热系统,建立了热水一级网("二环")运行调节曲线数学模型,从而实现"三环制"供热系统连锁调节和自动控制,达到最大限度的节能.     

Parlay的业务移动能力分析

基于Parlay业务规范的开放业务技术是下一代网络研究的重要内容。虽然Parlay业务规范目前已经被电信界普遍接受,但是,Parlay业务规范是否可以满足现在和未来电信业务发展的需求,这仍然是一个尚未解决的问题。论文通过构造两个典型的移动业务模型,并基于Parlay业务规范,设计了相应的实现模型,论证了Parlay业务规范对移动业务的支持能力。为了保证实现模型的正确性,论文采用通信顺序进程(CSP)理论描述了移动业务实现模型。最后得出结论:Parlay业务规范在跨业务管理域的业务调用、用户身份验证和用户业务逻辑传递方面仍然无法支持业务的移动性。     

含季节性虚拟变量的GM(1,1)模型及其应用

针对GM（1，1）模型难以准确预测季节性时间序列的问题，本文将季节性虚拟变量作为灰作用量引入传统GM（1，1）模型中，提出了含季节性虚拟变量的GM（1，1）模型（简记为GMSD（1，1））.在GMSD（1，1）模型定义型和白化型的基础上，推导了GMSD（1，1，x⁽¹⁾）模型、GMSD（1，1，x⁽⁰⁾）模型、GMSD（1，1，b）模型、GMSD（1，1，exp）模型、GMSD（1，1，C）模型等五种派生型GMSD（1，1）模型，构建了季节性虚拟变量GM（1，1）模型群.同时利用粒子群算法对GMSD（1，1）模型和GMSD（1，1，exp）模型中的解析式进行最优化求解.最后以中国水力发电量的季度数据为例，验证了含季节性虚拟变量的GM（1，1）模型及其派生模型的有效性和优越性.结果表明：相较于传统GM（1，1）模型，引入季节性虚拟变量的GMSD（1，1）模型及其派生模型更能准确地描述系统特征序列的季节性波动和周期性变化特征.     

加强实践教学提高人才培养质量

职业教育应重视培养学生的能力,必须加强实践教学,明确教学指导思想,强化实践教学环节,鼓励学生社会实践活动,抓好毕业实训工作。     

CD1d and natural T cells: how their properties jump-start the immune system

Cellular and humoral immune mechanisms recruited to defend the host from infectious agents depend upon the early immune events triggered by antigen. The cytokine milieu within which the immune response matures is the most important of many factors that govern the nature of the immune response. Natural T cells, whose function is controlled by CD1d molecules, are an early source of cytokines that can bestow type 1 or type 2 differentiative potential upon helper T lymphocytes. This review attempts to illuminate the glycolipid antigen presentation properties of CD1d, how CD1d controls the function of natural T cells and how CD1d and natural T cells interact to jump start the immune system. CD1d is postulated to function as a sensor, sensing alterations in cellular lipid content by virtue of its affinity for such ligands. The presentation of a neo-self glycolipid, presumably by infectious assault of antigen-presenting cells, activates natural T cells, which promptly release pro-inflammatory and anti-inflammatory cytokines and jumpstart the immune system. Received 10 July 2000; received after revision 16 October 2000, accepted 16 November 2000     

Sphingolipids in mammalian cell signalling

Sphingolipids and their metabolites, ceramide, sphingosine and sphingosine-1-phosphate, are involved in a variety of cellular processes including differentiation, cellular senescence, apoptosis and proliferation. Ceramide is the main second messenger, and is produced by sphingomyelinase-induced hydrolysis of sphingomyelin and by de novo synthesis. Many stimuli, e.g. growth factors, cytokines, G protein-coupled receptor agonists and stress (UV irradiation) increase cellular ceramide levels. Sphingomyelin in the plasma membrane is located primarily in the outer (extracellular) leaflet of the bilayer, whilst sphingomyelinases are found at the inner (cytosolic) face and within lysosomes/endosomes. Such cellular compartmentalisation restricts the site of ceramide production and subsequent interaction with target proteins. Glycosphingolipids and sphingomyelin together with cholesterol are major components of specialised membrane microdomains known as lipid rafts, which are involved in receptor aggregation and immune responses. Many signalling molecules, for example Src family tyrosine kinases and glycosylinositolphosphate-anchored proteins, are associated with rafts, and disruption of these domains affects cellular responses such as apoptosis. Sphingosine and sphingosine-1-phosphate derived from ceramide are also signalling molecules. In particular, sphingosine-1-phosphate is involved in proliferation, differentiation and apoptosis. Sphingosine-1-phosphate can act both extracellularly through endothelial-differentiating gene (EDG) family G protein-coupled receptors and intracellularly through direct interactions with target proteins. The importance of sphingolipid signalling in cardiovascular development has been reinforced by recent reports implicating EDG receptors in the regulation of embryonic cardiac and vascular morphogenesis. Received 16 May 2001; received after revision 29 June 2001; accepted 3 July 2001     

香港恒生指数期权市场的过度反应现象研究

论文以香港恒生指数期权市场为实证,对期权隐含波动率的“期限结构”进行了建模和研究。实证分析表明期权的隐含波动率具有很强的“中心回复”特性,那么,在有效市场假设和Black-Scholes期权定价模型正确性的前提之下,当一个期限较短的期权的隐含波动率发生变动时,另一个其他方面与之完全相同,但期限较长的期权,其隐含波动率的变动幅度应小于前一个期权隐含波动率的变动幅度。然而,通过对香港恒生指数期权市场的实证分析,两种不同统计检验方法的结果都对香港恒生指数期权市场的有效性假设提出了质疑－－市场并不是有效的基于理性预期的,而是存在着显著的“过度反应”现象。     

江泽民总书记“七一”讲话是对马克思主义建党学说的重大发现

20世纪90年代以来,以美国为首的西方自由世界,根据苏联解体的情况,加速分化瓦解社会主义中国的图谋,面对严峻挑战,中国共产党在邓小平理论指导下,实行改革开放政策,取得经济建设伟大成就。江泽民总书记“七一”讲话,站在时代的高度,高屋建瓴、总揽全局,就新世纪建设什么样的党,怎样建设党,提出了新要求,是新时期党建工作的纲领性献,“三个代表”的思想是党在新世纪的立党之本,执政之基和力量之源。     

Investigation on the coordinate structures of the rare earth metal complexes with edta and cydta ligands

0　IntroductionThegreatattentionhasbeenpaidtorareearthorradioactiverareearthmetalcomplexesfortheirvariousbiologicactivi ties[1 3] .Forexamples,theradioactiverareearthmetal1 53SmⅢ com plexeswerewidelyusedfortumourtherapyofbrain ,liver,lung ,heartandbonetissues[4 5] ;forrareearthmetalEuⅢandTbⅢemitinguniquefluorescence ,theircompoundswereappliedfordiagnosesofvariousdiseases[6 ,7] ;forrareearthmetalGdⅢcontainingthemosthigh spinsingleelectrons,itscomplexeswereusuallyusedforcontrastagentsofmag…