沙樱桃黄酮对SGC-7901细胞增殖的抑制作用

给予体外培养SGC-7901细胞沙樱桃黄酮,用克隆形成法和X射线能量色散谱仪,测定对细胞分裂增殖和细胞内微量元素含量的影响．结果表明,沙樱桃黄酮（O．1,0．5,5mg·L^-1）组和5-Fu组克隆数比对照组明显减少,抑制率分别为27．60%44．50％、66．60％和40．80％;细胞内Ca^2＋离子水平随给药浓度的增加而升高,K件和P件离子浓度则降低．     

Effect of high doses of somatostatin on adenylate cyclase activity in peripheral mononuclear leukocytes from normal subjects and from acute leukemia patients

Summary In normal lymphocytes somatostatin non-competitively inhibited basal (ID₅₀ 5×10^–4 M) and isoproterenol- and forskolin-stimulated adenylate cyclase activity (Ac). In acute leukemia blasts, non-responsive to isoproterenol, forskolin, which activates the catalytic subunit, stimulated and somatostatin inhibited Ac, thus indicating the leukemic enzyme, though defective, retains the inhibitory pathway and catalyst function.     

The effects of ethanol on embryonic actin: A possible role in teratogenesis

Summary When the neural crest is cultured in the long or short term presence of ethanol, monoclonal anti-actin reveals the development of a disorganized actin cytoskeleton. In the long term, many cells fail to differentiate morphologically, whereas in the short term already differentiated cells rapidly alter their shape and their cell-to-cell contacts.     

Retroviruses released from a human tumor xenograft in nude mice induce colony-stimulating factor (CSF) activity in human fibroblastic cells

Summary A human colony-stimulating factor (CSF)-producing tumor transplanted into athymic nude mice released retroviruses in vitro. The viruses induced CSF activity in human fibroblastic cell lines.     

Gametes contain angiotensin converting enzyme (kininase II)

Summary The localization of angiotensin converting enzyme (ACE) in the gonads of the normal rabbit was studied by immunofluorescence and immunoelectron microscopy. The enzyme is present in the cytoplasm of testicular spermatids and of epididymal and ejaculated spermatozoa, and on the surface of follicular and tubal cocytes. These findings support the hypothesis that ACE has a role in gamete maturation and in fertilization.Acknowledgments. This study was supported by NIH grant AM36807 and by grants from the American Heart Association, the Joe and Emily Lowe Foundation, and the Dana Foundation. We thank L. Fernandez, B. Luders, I. Pawlowski, and K. Schwegler for technical assistance.     

Serotonin is localized in endothelial cells of coronary arteries and released during hypoxia: A possible new mechanism for hypoxia-induced vasodilatation of the rat heart

Summary In this report we demonstrate the immunocytochemical localization of serotonin in endothelial cells of rat coronary vessels and a significant increase in the release of serotonin into the perfusate of Langendorff rat heart preparations during hypoxia. It is suggested that serotonin, localized in endothelial cells, is released during hypoxia and could provide part of a pathophysiological mechanism for vasodilatation to protect the heart from damage due to hypoxia.This research was supported by the British Heart Foundation and the excellent technical assistance of Jon Bokor is gratefully acknowledged.     

Super-high sensitivity systems for detection and spectral analysis of ultraweak photon emission from biological cell cells and tissues

Summary In this paper we summarize and discuss the modern technology and systems, studied and established by our research group, for performing the detection and special analysis incorporated with the super-high senstivity photon counting method for the study of ultraweak photon emission; for example, extra-weak bioluminescence and chemiluminescence from living cells and tissues, closely related to biochemical and biophysical processes and activities. An excellent sensitivity of the basic photon counting system, making it possible to achieve count rates in the very low range of one photoelectron per second to one per minute, allowed us to carry out in vivo as well as in vitro measurements, and analyses of ultraweak bioluminescence and chemiluminescence. Recent results concerning ultraweak photon emission from blood samples and organ homogenates of rats are presented and reviewed as one of the interesting and valuable applications of our modern technology for studying ultraweak cell and tissue radiation.     

Pineal function cannot prevent the occurrence of castration cells in male rats

Summary The relationship between the pineal gland and the pituitary gland was investigated in male rats. The results indicate that the hypothalamo-adenohypophysial-gonadal axis is affected by the pineal gland, but the appearance of castration cells following gonal ablation may be only slightly modified by alterations in pineal gland function.     

米曲霉自溶作用的研究Ⅱ自溶过程中细胞物质及酶活的变化

在米曲霉最佳的自溶条件下，对其自溶过程中重要的细胞物质及酶进行了测定，结果自溶液中测出１７种氨基酸酸，总氨基酸释放率为６８．７９％，核酸释放率为５０．００％，糖的释放率为２４．５８％，酸性蛋白酶活性高，而５’－磷酸二酯酶活性较低。