Cationic polymeric nanoformulation: Recent advances in material design for CRISPR/Cas9 gene therapy

CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/clustered regularly interspaced short palindromic repeat associated proteins 9) gene editing platform is a promising therapeutic tool for genetic disorders, due to its ability to manipulate the pathogenic gene in genomic level and to easily target specific gene by manipulating single-guide RNA. However, its successful delivery remains a challenge. Up to now, great efforts have been made to explore an effective strategy for CRISPR/Cas9 delivery. But among those delivery methods, physical methods are mainly operated on cultured cells thus limited to laboratorial use; viral vectors are hindered by fetal immunogenic and carcinogenic effects thus dubious in clinical application. Therefore, cationic polymeric vectors, with the ability to interact with CRISPR/Cas9 system to form a nanoformulation as a non-viral approach, are attracting increasing attentions, due to advantages such as well protection of cargos, less limitation in payload size, low immunogenicity or carcinogenicity, potential modifications for further functions, and ease in mass production. In this review, the recent discoveries on polymeric vectors utilized in delivery of CRISPR/Cas9 system will be summarized. With emphasis on advanced features of those polymeric vectors or their nanoformulations to meet the demands of different CRISPR/Cas9 delivery forms (plasmid, mRNA or protein), the detailed illustrations on their disease treatment applications, such as cancer, diabetes or antibiotic-resistant infections, will also be reviewed.     

谈全方位品牌管理与创名牌

提出了“全方位品牌管理”一词，将品牌管理从传统营销决策领域延伸到企业的生产经营全过程．文章从我国名牌产品市场存在的问题入手，详细阐述了实施全方位品牌管理的意义和措施．     

加速度传感器在模型火箭定高分离中的应用

模型火箭定高分离是航天模型比赛的重要比赛项目之一,其难点在于高度的精确控制。为了使模型火箭能在设定高度分离,系统采用飞思卡尔MC9S08AW60单片机和集成3轴加速度传感器MMA7455模块,通过SPI数据传输协议将加速度传感器测得值传至单片机进行变加速度的二次积分运算求得高度值。实验测试结果表明,能完成对模型火箭高度的测定与其在定高分离的功能,其定高精度指标符合模型火箭定高分离系统的应用要求。     

利用Multisim9进行电子电路仿真的教学研究

根据电子电路实验在学习本课程中的重要性，提出了在教学中利用Muhisim9进行仿真、分析与设计的思路．此软件的优点是灵活方便、实验速度快、效率高，克服了传统实验与实验室开放的局限．通过一个实例进行了阐述，从而促进理论教学，改善实验教学效果．     

蒽环类叔胺的合成与表征

以9-蒽甲醛为原料，和苄胺、芳香胺在冰乙酸的催化下脱水生成席夫碱，经过硼氢化钠还原，和3，5-二烷氧基苄基氯发生亲核取代反应，合成了一系列蒽环类叔胺化合物，并通过NMR、质谱对其结构进行了表征．     

Mutations in Hemagglutinin of a Novel Avian-Origin H7N9 Virus That Are Critical for Receptor Binding Specificity

A novel avian-origin H7N9 influenza virus was discovered in March in China and has caused a total of131 people infected including 39 deaths in China as of June 9, 2013. Adaptation of avian viruses to efficiently infect humans requires the viral hemagglutinin（HA） binding switches from avian to human type receptors with help of some mutations in HA. As such it is critical for pandemic assessment to discover these mutations as hallmarks of adaptation. To continue our previous study of this novel H7N9 virus, we identified two sets of mutations in HA. The first set of mutations are present in the current circulating strains of 2013 H7N9 in China, and the second set are potential mutations that were found when compared to the HAs of previous human H7 subtype. These two sets of mutations exhibited unique features. The first group of mutations, on average, enhanced the HA binding to human type receptors whereas reduced that to avian types. Further the reduction of avian binding was almost three times of the increase of the human binding. The second group increased the binding to both human and avian types.But the increase in human types was almost three times of that in the avian types. Though different in their way of changing the binding preference, these two sets of mutations both contained more mutations to decrease the avian binding and increase the human binding than those that did the opposite. Our research highlighted the pandemic potential of this novel virus by showing the important mutations that could potentially help it to adapt to human hosts. Our findings offered new insights into the current state of evolution of this virus, which might be helpful for the continued surveillance of the emergence of H7N9 strains having the ability of human-to-human transmission.     

连铸中间包分流挡渣实验研究

在中间包流场分布分析的基础上，借助水模拟试验研究了6流小方坯连铸中间包内钢水流动规律。通过设置分流挡渣墙改进中间包内的钢水流动，分离钢水和炉渣，提高钢中夹杂物的上浮率，达到了预期的效果，并应用于生产实践．     

泡沫沥青二次冷再生混合料不同稳定剂的应用

分别采用泡沫沥青与水泥作为稳定剂对泡沫沥青冷再生路面进行二次冷再生试验研究.研究表明,以泡沫沥青作稳定剂进行二次冷再生混合料设计形成的泡沫沥青二次冷再生混合料,其抗拉性能、水稳性能与二次冷再生混合料中细料含量有关;高温稳定性能与铣刨料用量、铣刨料中沥青老化程度以及二次冷再生混合料中细料含量有关.以水泥作稳定剂进行二次冷再生混合料设计,采用7d无侧限抗压强度能够很好地确定新添加骨料比例,适量的新添加骨料能明显增大二次冷再生混合料的7d无侧限抗压强度.根据室内研究成果,对试验路段进行辅筑,证明两种二次冷再生技术可行,辅筑路段路用性能良好.     

Cu48Zr43Al9合金在0.05mol/L Na2SO4介质中腐蚀电化学行为研究

采用真空电弧熔炼法制备Cu48Zr43Al9晶态合金及铜模铸造法制备Cu48Zr43Al9非晶合金。通过极化曲线和交流阻抗谱技术研究了Cu48Zr43Al9晶态和非晶态合金在0.05 mol/LNa2SO4溶液中腐蚀电化学行为。结果表明,在0.05 mol/LNa2SO4溶液中,Cu48Zr43Al9晶态和非晶态合金的交流阻抗谱均呈单容抗弧,实验结果说明合金的腐蚀过程是由电化学控制。与非晶合金相比,腐蚀电位负移,晶态合金的电荷传递电阻减小,腐蚀电流密度变大,腐蚀速度加快。在0.05mol/LNa2SO4溶液中,Cu48Zr43Al9非晶态合金具有良好的耐腐蚀性能,这是因为非晶态合金不具有晶格缺陷,结构均一稳定。     

太阳光紫外线照度计的设计

采用MC9S12XS128单片机作为核心控制芯片,利用紫外线传感器G5842进行信号采集,进行了硬件电路设计,完成了紫外线照度计的设计,通过软件编程,实现了对太阳光紫外线的检测并实时显示,通过实验验证了方案的可行性,