Isolation of human epidermal stem cells by adherence and the reconstruction of skin equivalents

The isolation of human epidermal stem cells is critical for their clinical applications. In the present study, we isolated three populations of epidermal keratinocytes according to their ability to adhere to collagen type IV: i.e., rapidly adhering (RA), slowly adhering (SA), and non-adhering (NA) cells. The aim of this study was to characterize RA cells and to investigate the possibility of using these cells for epidermis reconstruction. To identify RA cells, flow cytometric analysis was performed using anti-₆ integrin and anti-CD71 antibodies. RA cells express high levels of ₆ integrin and low levels of CD71, which are considered as markers of an epidermal stem cell nature. Furthermore, electron microscopy showed that RA cells are small and have a high nuclear to cytoplasmic ratio, whereas SA and NA cells have well-developed cellular organelles and abundant tonofilaments. Western blot analysis showed that RA cells are slow cycling and express p63, a putative epidermal stem cell marker, whereas SA and NA cells express c-Myc, which is known to regulate stem cell fate. To compare epidermal regenerative abilities, skin equivalents (SEs) were made using RA, SA, and NA cells. The epidermis constructed from RA cells was well formed compared to those formed from SA or NA cells. In addition, only SEs with RA cells expressed ₆ integrin and ₁ integrin at the basal layer. These results indicate that RA cells represent epidermal stem cells and are predominately comprised of stem cells. Therefore, the isolation of RA cells using a simple technique offers a potential route to their clinical application, because they are easily isolated and provide a high yield of epidermal stem cells.Received 2 July 2004; received after revision 20 August 2004; accepted 10 September 2004     

f—M—余正则序列和f—余模

在对模的正则序列、余正则序列及f-正则序列性质学习与研究的基础上，定义了拟局部环（A,Ψ）（Ψ为A的唯一极大理想）上Artin模M的f-余正则序列并给出了一些简单性质，由此定义了一类模－f-余模，并将f-余正则序列用于一类特殊的广义分式模，得到了一个正合性原理。     

因子与奇偶性

本文推广了(g,f)一因子的概念,对次限制子图进一步加进了次的奇偶性限制。主要结果是-个图含有这种奇偶性限制子图的充分必要条件。这个条件推广了Tutte和Lovasz关于f-因子和(g,f)-因子的有关定理。     

Matrix equation and its application to classification of finite-dimensional estimation algebras

Under a very natural condition, a matrix equation is proved to have only trivial solution. This result is then applied to the classification problem of finite-dimensional estimation algebras, which gives a simpler proof of Tang s recent result on the constant structure of the O~matrix.     

轴矢量介子f1(1282)在手征孤立子模型中的效应--f1运动方程的大NC分析

从U (2 ) L ×U (2 ) R 手征孤立子模型出发 ,通过(πωf1 )模型考察了轴矢量介子 f1 (1 2 82 )的时间分量和空间分量的量级与其手征伙伴ω的关系。通过对 f1 运动方程的大NC 分析 ,发现 :f1 的时间分量 f0 具有O(1N3/2C)的量级 ,其空间分量 fi 具有和ω空间分量ωi 相同的O(1NC)量级。因此 ,在研究孤立子转动激发时 ,fi 和ωi 应该同时考察。这是首次对于 f1 在手征孤立子模型中的效应进行定量估算。     

关于R分离空间的注记

在紧致空间和Lindel(o)f空间中分别讨论了R分离空间,得到了一些有用的结果.     

内蒙古葱属一新变型及其花粉形态

分类号Ｑ９４９文献标识码Ｂ文章编号１００１－８７３５（１９９９）０１－００６２－６３白花蒙古韭新变型ＡｌｉｕｍｍｏｎｇｏｌｉｃｕｍＲｅｇｅｌｆ．ａｌｂｉｆｌｏｒｕｍＹ．Ｂａｏｆ．ｎｏｖ．Ａｔｙｐｏｄｉｆｅｒｔｐｅｒｉａｎｔｈｉｓｅｇｍｅｎｔａａｌｂａ...     

大茴香有效成分提取条件优化研究

采取水蒸气蒸馏法和索氏提取法,分别对大茴香提取从时间、破碎度、料剂比和乙醇浓度等方面进行条件优化,以精油的得率为指标,选用正交表L9(34)设计三因素三水平九个实验.结果表明:索氏提取法优于水蒸气蒸馏法,且在索氏提取法中,将大茴香干果粉碎过20目筛,加10倍量95%乙醇,回流提取3h,得油率最高,可达0.185%。     

INDO近似下包含d,f轨道的定域化分子轨道方法

研究了当基组包括d、f原子轨道时,保证INDO方法具有旋转不变性的两种方法——估算全部杂化积分和权重平均库仑交换积分。导出了INDO近似下Edmiston-Rue-denberg能量定域化方法的LCAO计算公式,建立了相应的计算程序并对程序的使用进行了考验和说明。     

浅谈车轮定位

为了适应汽车高速化的需要，满足汽车行驶平稳、乘坐舒适的要求，各种新技术和新结构被不断地应用在现代汽车上。从主销后倾角、主销内倾角、前轮外倾角、前束等方面对现代汽车与传统汽车的车轮定位的变化进行了比较。