探针N-戊基-N'-(对氨基苯磺酸钠)硫脲快速测定血清样品中蛋白质含量的光谱法研究

研究了N-戊基-N'-(对氨基苯磺酸钠)硫脲(APT)与人血清白蛋白(HSA)体系的三维荧光光谱,证明了两者可以发生相互作用,APT的加入导致HSA疏水微环境极性和构象的变化.考察了△λ值,pH值、离子强度、试剂加入顺序及APT用量等因素对APT与HSA体系的同步荧光强度的影响.在最佳实验条件下,体系的同步荧光强度与H...     

扫描探针显微镜图像处理通用软件包的设计

扫描探针显微镜(SPM)是固体物体表面结构分析的重要手段.采用Visual Basic结合C语言编写动态链接库的方式设计了SPM图像处理的通用软件包.该软件包括SPM图像的显示、漂移校正、畸变校正、剖面线分析、傅里叶变换分析等,有效消除外界环境和仪器自身对SPM图像的干扰,丰富了SPM图的信息,并克服商品化仪器软件通用性差的缺点.     

用三维原子探针研究压力容器模拟钢中富铜原子团簇的析出

用三维原子探针（3DAP）和热时效处理方法研究压力容器模拟钢中富铜原子团簇的析出过程.提高了Cu含量的压力容器模拟钢样品经过880 ℃加热淬火后,在400和500 ℃进行了不同时间的时效处理,显微硬度测试结果表明,在400和500 ℃时效的过程中硬度峰值分别出现在100和5 h.3DAP分析结果显示,样品在400 ℃分别时效100,150和300 h后,富铜原子团簇的数量密度是递增的,从1.5×10²³ m^-3增加到6.2×10²³m^-3,但富铜原子团簇的长大非常缓慢,团簇的最大等效直径只从2 nm增大到了3.5 nm,团簇中的Cu原子数分数 x 为20%,还含有Mn和Ni,并且观察到Mn和Ni在团簇和基体金属的界面处发生明显的富集.     

超声流量计探头安装入侵长度对测量影响的估算

侵入式探头对超声流量计流场产生扰动．探头扰动造成测量误差,制约非实流标定的发展．针对探头扰动提出简化模型,根据简化模型给出探头扰动误差估算方法．与DN500和DN100两种口径的多声路超声流量计实流实验结果进行对比,估算结果与实验结果相差最大不超过0．4％．实验结果表明,随超声流量计直径减小,探头扰动造成的测量负误差迅速增大．DN100多声路超声流量计中探头扰动造成的测量误差达到-5％,且误差随雷诺数的变化率比DN500多声路超声流量计大．     

强制润滑状态下齿轮传动装置的热平衡

介绍一种新型激光非接触位置测量传感器和相应的硬、软件系统，该激光测头有效克服了同类非接触式测头线性度差、存在阴影效应等缺点．采用的专用线阵ＣＣＤ数据采集电路和相应的处理方法，显著地提高了激光测头的测量速度和精度．该激光测头在华中Ⅰ型数控加工中心上成功地实现了未知曲面的数控自动测量．     

基于磁性荧光双探针基础上的2,4-二氯苯氧乙酸残留的快速免疫检测体系的建立(英文)

建立了一个基于磁性荧光双探针基础上的免疫快速检测体系,以实现液相中快速检测食品中2,4-二氯苯氧乙酸(2,4-D)残留.该体系将2,4-D抗体结合Fe3O4@SiO2-NH2得到的复合物作为磁性探针和固相载体,2,4-D-OVA被标记CdTe@SiO2-NH2作为荧光探针以产生荧光信号,通过荧光探针与磁性探针复合物与2,4-D抗体竞争结合实现免疫快速检测.探讨了荧光探针最佳优化条件,在p H值8.2,2,4-D-OVA加入量为500μL,偶联时间为70 min时,偶联得到的荧光信号最强,双探针检测后得到该检测体系最低检测限为3.55×10-8.得到金磁、量子点荧光双探针免疫系统,绘出标准曲线,得到最低检测限达3.55×10-8.该检测体系与传统ELISA方法相比,可以大大缩短检测时间,放大检测信号.     

Investigation of self-assembled protein dimers through an artificial ion channel for DNA sensing

Artificial nanopores have become promising tools for sensing DNA. Here, we report a new technique for sensing DNA through a conical-shaped nanopore embed- ded in track-etched polyethylene terephthalate （PET） membrane. Two different streptavidin-conjugated mono- valent DNA probes were prepared that can bind to two distinct segments （at either end） of the target DNA. The size of target DNA-linked to the two streptavidin-conju- gated monovalent DNA probes is double that of the indi- vidual probes. By precisely controlling the tip diameter of the conical nanopore embedded in the PET polymer, events due to the translocation of the streptavidin-conjugated monovalent DNA probes through the nanopore can be fil- tered and purposely undetected, whereas the current pulses due to the translocation of the target DNA-induced self- assembled complexes can be detected. The two streptavi- din-conjugated DNA probes cannot be linked by multi- mismatched DNA. Therefore, multi-mismatched （non- specific） DNA will not induce any current pulse signatures. The current pulse signatures for the self-assembled com- plex can be used to confirm the presence of the target DNA. The size-dependent detection of self-assembled complexes on the molecular level shows strong promise for the detection of biomolecules without interference from the probes.     

基于数学形态学的触针重建

讲述在接触式测量时触针产生图像失真的原因，然后在数学形态学的基础上介绍了一种触针重建的方法来减少触针在测量中的影响．     

大肠杆菌yigP基因启动子的定位

在已知大肠杆菌yigP基因的最小功能片段为yigP-P4P2基础上,将该片段装载于无外源启动子的载体质粒上,通过功能回补yigP基因缺陷株JDP14,发现其可以代替温敏质粒,保证大肠杆菌正常生长,推断其包含完整转录单元。RT-PCR结果显示该片段内部有转录产物,即yigP-P4P2片段具有转录独立性。将不同长度的yigP-P4P2亚片段克隆到启动子探针质粒pSP-Z上,通过对重组菌进行蓝白斑筛选及β-半乳糖苷酶活性测定,结果显示pP40V3-Z/JM83和pP40V4-Z/JM83可观测到蓝色菌斑,并检测到较弱的β-半乳糖苷酶活性,由此表明大肠杆菌yigP基因启动子位于该片段上游,下游边界位于引物V4区域内。     

The influence of probe lift-up height on CNT electrical properties measurement under EFM DC mode

Nowadays, one of the bottlenecks which hinder the development and application of carbon nanotube （CNT） nano device is that no pure semiconducting CNT （s-CNT） or metallic CNT （m-CNT） can be obtained, and for solving this problem scientists proposed some methods on preparation or separation, but all the results still should be detected and feedback to the process for further improving the preparation and separation methods. Thus, it is very important to measure and distinguish the electrical properties of CNT. For that, scientists proposed a method to measure CNT electrical properties based on DC elec- trostatic force microscope （EFM） mode, which distin- guishes m-CNT from s-CNT according to different scan line shape to CNT with different electrical properties. But, we discovered that the probe lift-up height will seriously affect the shape of the scan line, which makes this method not reliable in distinguishing m-CNT from s-CNT. In this paper, the authors deeply researched the influence of probe lift-up height and also gave corresponding theoretical analysis and explanation, which will greatly improve the method of detecting CNT electrical properties by EFM.