猕猴桃蛋白酶在胍中“慢构象快活力变化”现象

比较了猕猴桃蛋白酶(Actinidin)在盐酸胍中变性时的荧光变化与活力变化的关系。当胍浓度为0.1 mol/l时,酶的最大荧光发射波长λ_(max)不变,荧光强度上升。此时酶被激活,活力提高25％,激活速度比变性速度快5.0倍。胍浓度逐渐增大,酶λ_(max)略红移,4.0 mol/l时达336 nm。但荧光强度降低,酶表现为快相和慢相失活的二个一级反应,失活速度常数比变性速度常数快 1～3个数量级。Actinidin 的激活与失活现象显示这可能与活性部位相关的 Trp 微环境的构象变化有关。胍变性结果指出 Actinidin 构象变化速度小于活力变化速度,这代表酶变性反应时慢构象变化快活力变化的一种模式。     

咖啡因对侧耳电离辐射损伤的效应

侧耳双核菌丝经γ射线辐照后用咖啡因处理，其生长速度和漆酶及多酚氧化酶活性较单一γ射线辐照明显降低，使电离辐射损伤加剧；辐照前用咖啡因处理，抑制辐射损伤修复的效果优于辐照后处理。     

原生质体诱变选育分解X光胶片的菌株

从厦门福达感光胶片厂的污水中分离到207株芽孢杆菌,其中一株显示了较强的分解明胶的能力.该菌经鉴定为巨大芽孢杆菌(Bucillus megaterium 207).用溶菌酶制备207菌的原生质体,经紫外线诱变获得一变异株(207—A5),产蛋白酶活力由830u/ml 提高到4731u/ml.     

猴头菌原生质体制备条件的研究

比较了不同酶液、酸碱度，渗透压稳定剂，菌龄和菌丝培养基成分等因素对猴头菌菌丝释放原生质体作用的影响。结果表明：在土豆培养基（ＰＤＰ）上静止浅层培养９６小时的菌丝，用２％的溶壁酶液，以０．６ｍｏｌ／ＬＭｇＳＯ４为渗透压稳定剂，在ＰＨ５．５的条件下酶解，获得的原生质体数最大。     

紫外线诱变侧耳菇担孢子的研究

对侧耳菇 99号担孢子 ,采用不同时间的紫外线照射诱变 ,分离纯化后 ,得到在遗传上相对稳定的突变菌株———侧耳菇 99号V0 2 .该菌株在综合PDA斜面培养基上生长速度快 ,菌体浓密洁白 ,较对照菌株性状优良 .     

平菇ACCC50596菌株液体菌种培养研究

采用单因子试验统计方法对平菇ACCC50596菌株液体菌种培养的培养基和培养条件进行了优化筛选，获得了液体菌种的培养基最佳配方和最佳培养条件．     

Fe3O4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration

Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed.     

青蘑菌种分离与培养特性研究

以新鲜野生青蘑子实体为材料,用组织分离法获得青蘑菌株。研究了该菌株的培养特性。结果表明,青蘑菌丝生长的适宜pH为6.5,适宜温度为25℃,适宜氮源是蛋白胨,适宜碳源是玉米粉,适宜栽培培养基是棉籽壳培养基。     

紫外诱变原生质体提高脂肪酶活力

以粗壮假丝酵母CJ-1为出发菌株,对其进行原生质体紫外诱变选育.筛选得到10株脂肪酶活力比出发菌株高的突变株,其中菌株CJ-1-09的酶活达35.78 U/mL,比CJ-1提高了4.58倍.突变株CJ-1-09经10次传代,其脂肪酶活性保持稳定.