甲烷八叠球菌(Methanosarcina sp.)若干特性的研究

利用甲醇、H_2 CO_2或乙酸盐作为碳源和能源,从池塘的沉积物取样,经过反复的富集和分离,得到了甲烷八叠球菌的纯培养物。该菌在H_2 CO_2或甲醇上生长最快,1～2周出现菌落而在乙酸盐上3～4周出现菌落。菌落直径通常为1～2mm,在培养基的深层为不规则的多角形,表面菌落为圆形。菌落颜色为白至黄色。特殊的假八叠形使之很容易鉴定到属的水平。甲烷八叠球菌在液体培养基或固体培养基中均产生甲烷。酵母膏对该菌的生长具有很大的刺激作用。在420 nm处该菌产生荧光。     

Cu(Ⅱ)促进海洋真菌Fusarium sp.ZZF51产生代谢产物二（5-丁基-2-吡啶甲酸-N1,O2）合铜(Ⅱ)的研 究

 从南海红树林内源真菌Fusarium sp.ZZF51的培养液中分离得到一金属络合物（1）,通过波谱数据和单晶衍射数据解析其结构为二（5-丁基-2-吡啶甲酸-N1,O2）合铜(Ⅱ)。为提高其产量,研究了Cu(Ⅱ)对络合物（1）产量和菌株生长的影响,结果表明：在参与真菌Fusarium sp.ZZF51代谢活动的过程中,Cu(Ⅱ)能显著促使络合物（1）的产生,但对真菌的生长影响甚微。     

一株高效高温菌的筛选初步鉴定和性质研究

从高温堆肥物料中通过平板培养法分离、纯化并筛选出一株高效高温菌YB16.YB16具有同时分解淀粉、蛋白质、油脂和纤维素等大分子有机物的能力.经鉴定YB16为革兰氏阳性,杆状,有芽孢,好氧, G+Cmol%为50.66%,初步鉴定YB16属于芽孢杆菌属.pH生长范围为6.0～9.0,最适生长温度为65 ℃左右.     

多氯联苯降解菌Rhodococcus sp．RHA1的功能基因

概述了多氯联苯（Polychlorinated biphenyls,PCBs）降解菌-Rhodococcus sp．RHA1的功能基因研究,包括bphACB基因、etbAC基因、ebdA基因和bphDEF基因的结构和在降解途径中的作用和表达．     

Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp. PCC 6803

From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (fbp1), which encodes the fructose-1,6-biphosphatase (FBPase) / sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacked in slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803.     

Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp. PCC 6803

From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (fbp1), which encodes the fructose-1,6-biphosphatase (FBPase) / sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacked in slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803.     

Mycelial fermentation and medicinal properties of a valuable medicinal fungus Cordyceps sinensis Cs-HK1

Cordyceps sinensis （Cs） or Dongchongxiacao in Chinese is a rare and precious medicinal mushroom. In this work, a Tolypocladium sp. fungus named Cs-HK1 was isolated from a natural Cs fruiting body, and its ultraviolet （UV）, infrared （IR） and high-performance liquid chromatography （HPLC） spectra all exhibited high degrees of matching to those of natural Cs, indicating the similarity of chemical composition. A crude polysaccharide （PS） fraction isolated from Cs-HKI fungal mycelium showed significant antitumor activity, inhibiting melanoma tumor growth in mice. The PS-treated mice showed significantly higher serum interferon-y （IFN-y） level and higher proliferation rate of lymphocytes ex vivo than the control animals, suggestive of the immunomodulatory activity of the PS fraction. In cell cultures, the PS fraction caused a significant and dose-dependent inhibitory effect on the proliferation of two cancer cell lines, B16/neu mouse melanoma and MDA-MB-231 human breast cancer cells, but not on normal spleen lymphocytes in vitro. Most of these bioactivities of the Cs- HK1 fungus were comparable to or even greater than those of natural Cs. The results showed that Cs-HK1 fungus may be a functional substitute for the natural Cs in health food and herbal medicine.     

鱼腥藻PCC7120α质粒上的parD/E同源基因all7155/asl7156的克隆及表达

为研究鱼腥藻PCC7120质粒上毒素抗毒素系统的蛋白质性质和相互作用,根据NCBI中PCC7120的α质粒上的all7155和asl7156基因数据,通过降落PCR克隆了目的基因(all7155/336 bp,asl7156/258 bp).将目的基因片段连接至p MD18-T构建了克隆载体,蓝白斑筛选了阳性克隆,经双酶切纯化后将目的基因连接至表达载体p ET30a(+),并转入表达菌BL21中,测序后证实构建成功,并利用IPTG诱导进行了表达.通过NCBI的BLAST蛋白比对,发现了all7155和asl7156与已知的Par D/E毒素抗毒素系统同源,可通过对此基因的克隆,表达和蛋白性质来进一步认识Par D/E系统.     

固定化菌株DF51对小麦幼苗的苯酚污染修复

为探讨固定化菌株DF51对苯酚污染土壤中小麦幼苗生长的影响,采用随机区组试验设计,在大田条件下研究施用不同质量浓度的苯酚（0、50、100、150、200μg/g）和固定化菌株DF51（0、10、20、30、40 kg a.i./hm2）对小麦的影响.结果表明：苯酚显著降低小麦的光合特性和抗逆性;固定化菌株DF51对苯酚土壤污染具有修复性能,且能提高苯酚胁迫下小麦的光合特性和抗逆性;P1D1和P2D2处理可恢复苯酚胁迫下小麦的生理特性.可见,固定化菌株DF51能显著提高小麦在苯酚胁迫下的耐性,这与其提高抗氧化酶活性有关.     

庙前井田童子岩组与文笔山组地层的划分

福建庙前井田属福建二叠纪含煤地层连城区,童子岩组的第一段、第二段的沉积特征与文笔山组相似,均为黑灰色泥岩、粉砂岩,地层难以划分,特别是出现缓断层构造时无法进行对比。野外工作中,笔者在文笔山组地层中发现假海乐菊石幼年种这一标志,作为划分依据。