LPS和IL-2对合浦珠母贝血细胞iNOS活性的影响

建立了一种合浦珠母贝血细胞原代培养方法,为贝类免疫学研究提供实验平台。通过台盼兰染色法检测细胞活力,结果显示:7d内细胞生长状态稳定,并且培养基中的FBS对细胞生长活力没有显著影响。以细胞中iNOS活性为检测指标,详细研究了LPS和IL-2对合浦珠母贝血细胞的激活作用。结果表明:LPS和IL-2对iNOS活性的诱导作用表现出浓度和时间依赖的特征。刺激后36h左右iNOS活性达到最大,LPS和IL-2的最佳诱导剂量范围分别为1～10g/mL和10～100ng/mL,并且IL-2对iNOS活性的激活作用较LPS快。     

Molecular Cloning and Distribution of a Plasma Membrane Calcium ATPase Homolog from the Pearl Oyster Pinctada fucata

Plasma membrane calcium ATPaso (PMCA) plays a critical role in transporting Ca2 out of the cy- tosol across the plasma membrane which is essential both in keeping intracellular Ca2+ homeostasis and in biomineralization.In this paper we cloned and localized a gene encoding PMCA from the pearl oyster Pinctada fucata.This PMCA shares similarity with other published PMCAs within the functional domains.Reverse transcdption-polymerase chain reaction analysis shows that it is expressed ubiquitously.Furthermore,in situ hybridization reveals that it is expressed in the inner epithelial calls of the outer fold and in the outer epithelial calls of the middle fold,as well as the edge near the shell,which suggests that PMCA may be involved in calcified layer formation.The identification and characterization of oyster PMCA can help to further under-stand the structural and functional properties of molluscan PMCA,as well as the mechanism of maintaining Ca2+ homeostasis and the mechanism of mineralization in pead oyster.     

合浦珠母贝幼苗的正常生长

调查珍珠养殖合浦珠母贝人工培育幼苗的管养和生长情况,认为合浦珠母贝的幼苗生长正常,不存在“贝种退化”的影响,成活率和生长率的提高,明显是由于幼苗营养方法的改良。