脂肪酶产生菌Serratialiquefaciens的分离及其酶性质的研究

从油污土样中分离到一株高产胞外碱性脂肪酶的细菌，经鉴定为液化沙雷氏菌。发酵上清液经超滤浓缩，ＤＥＡＥ纤维素层析，透析去盐后 冻干得精酶制品。表面活性剂吐温２０对酶反应也有一定激活作用。液化沙雷氏菌脂肪酶的研究，目前国内外均未见报道。     

生物操纵法治理富营养化湖泊的细菌类群研究

从污染水体中分离筛选出高效降磷除氮菌共９５株，经分类鉴定为假单胞菌属（Ｐｓｅｕｄｏｍｏｎａｓ），芽孢杆菌属（Ｂａｃｉｌｌｕｓ），肠杆菌科（Ｅｎｔｅｒｏｂａｃｔｅｒｉａｃｅａｅ）和硝化细菌科（Ｎｉｔｒｏｂａｃｔｅｒａｃｅａｅ）．经降磷测定实验表明，降磷率高达３１％～８０％的菌株占总磷细菌数的４４％．将分离的菌株按不同比例组合投入装有富营养化湖水和底泥的模拟装置中进行降解实验，结果总氮下降最高达８９．９％，总磷下降最高达９０．６％，同时浮游动物数量大为增加．说明物质正沿食物链流动．     

煤气化废水酚降解细菌的筛选及生长条件研究

从兰州煤气化排污口和泥土中取样,筛选出具有降解酚生理功能的细菌１２株,通过最佳营养配方选择和生长条件的研究,在实验室条件下,其中ＧＫ８２,ＧＫ１５０,ＧＫ１３４菌株在３５℃,１０８γ／ｍｉｎ旋转式摇床振荡培养２４－２８ｈ后,将煤气化废水中的６４１．９ｍｇ／Ｌ的酚降解,去除率达９８％。     

磁场对紫色非硫光合细菌脱色酶的影响

本文采用在紫色非硫光合细菌(简称PSB)的培养液中加入钡铁氧体磁粉的方法研完了磁场对PSB脱色酶活力的影响。结果表明,磁场作用于PSB表现出有益的生物效应,可提高脱色酶活力,增加脱色速度。     

Direct observation of the clockwise light-driven rotation of F<Subscript>0</Subscript>F<Subscript>1</Subscript>-ATP synthase complex

The purified thermophilic bacterium PS3 F₁ β _10×His-tag is inserted into the F_oF₁-ATP synthases of chro-matophores isolated from photosynthetic bacteria Rhodo-spirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protons-driving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by video-microscopic experiment, are connected via Maleimido-C₃-NTA to the reconstituted β_10×His-tag of F_oF₁-ATP synthases. The clockwise rotation of F_oF₁-ATP synthases driven by light is observed directly when viewed from the F_o side to F₁. This system should be valuable for further studying the coupling property of F_oF₁-ATP synthase.     

三种褐藻乙醇提取物抗动植物病原菌活性

采用纸片法对3种褐藻的乙醇提取物进行抗动植物病原菌的实验.结果表明:3种褐藻的抗细菌活性强于抗真菌活性.大部分薄层层析分离组分的抗菌活性均强于未分离前的海藻粗提物.3种褐藻均对3种鱼病病原菌具有抑菌活性,其中以羊栖菜的抑菌效果为最好.其具体的活性物质还有待于作进一步的研究.     

Population dynamics and spatial distribution of microbial species in multispecies biofilms under the action of direct electric current

The metabolism, population dynamics and spatial distribution of nitrifying bacteria and heterotrophs in biofilms under the action of direct electric current were investigated by using the micro-slicing technique. The nitrification rate of nitrifying bacteria was severely inhibited by a current over 10 Am^-2 at lower C/N ratios. Compared to heterotrophs, the nitrifying bacteria in the surface biofilms were severely inhibited, resulting in a significant decrease in bacterial density. An increase in current density narrowed the less current-sensitive inner biofilm region, and in addition the density of NO2-oxidizers decreased more significantly than that of NH4-oxidizers in the surface biofilms probably due to electrochemical reactions at the anode. However, the effect of current on both the population dynamics and the spatial distribution of the microbial species was less significant at larger C/N ratios.     

海洋细菌1202菌株产胞外多糖的适宜条件

应用响应面法(ResponseSurfaceMethod,RSM)对海洋细菌1202菌株胞外多糖发酵培养基进行优化.结果表明,适宜的发酵培养基组成为:蔗糖4%,酵母膏0.05%,玉米浆1.5%,CaCO30.15%,KH2PO40.08%,MgSO4·7H2O0.05%,FeSO4·7H2O0.01%,NaCl0.1%,pH7.5.在装液量为50mL(500mL三角瓶),接种量5%时,于28℃摇瓶培养7d,胞外多糖的产量可达到8.3mg/mL,转化率为20.75%.     

东湖细菌生理群间的相关性和优势生理群的分析

研究了武汉东湖异养菌（ＨＢ）及７种细菌生理类群（产淀粉酶细菌（ＡＢ）、产蛋白酶细菌（ＰＢ）、产脂肪酶细菌（ＬＢ）、有机磷细菌（ＯＰＢ）、无机磷细菌（ＩＰＢ）、硝化细菌（ＮＢ）及反硝化细菌（ＤＮＢ））间的相关性。统计分析表明,ＨＢ、ＡＢ、ＬＢ、ＰＢ、ＤＮＢ彼此呈正相关,而这几种类群与ＮＢ、ＩＰＢ、ＯＰＢ呈负相关。根据异养性细菌生理群与ＨＢ比值的百分率,ＬＢ可谓东湖春季优势生理群,ＡＢ为秋季优势生理群。