实现冠心病普查系统GISCAD的进化模型

一般说来,专家系统的开发是一个知识获取的过程。本文首先介绍了我们开发的一个冠心病普查系统,并讨论了知识获取和系统进化等问题,说明了用于开发冠心病普查系统的进化模型。     

分析记录磁头场的普适公式的探究

本文用 S—C 变换法,研究极尖为任意角度的磁头场,导出了一般实数型的理论公式,并对其应用作了讨论。     

MGB probe assay for rapid detection of mtDNAl1778 mutation in the Chinese LHON patients by real-time PCR

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.     

准确绘制在直线上的电荷系的电场线

用高斯定理求出了在直线上的点电荷系的电场线方程,提出由电场线方程绘制电场线的逐点计算——选点描绘法,给出用此方法绘制的一些电场线图形.     

云南壮剧的表演艺术形态

云南壮剧既借鉴了广戏等周边剧种的表演艺术，又吸收、融化了本民族的武术、舞蹈动作，还提炼、吸收了大量生活动作。在角色行当划分方面，云南壮剧经历了一个从无到有、由简到繁、繁简并存的历程，到解放前夕，富宁土戏、广南东路沙戏的角色行当划分比较细致，而广南北路沙戏、文山乐西土戏的角色行当划分则比较简略。     

中老年人身心健康与体育锻炼

研究体育运动对中老年人身心健康的作用，认为中老年人通过经常参加体育锻炼，可提高呼吸系统、血液循环系统及神经系统的机能，增强体质，提高健康水平及新陈代谢能力，延续衰老过程，延长寿命。     

浅谈树脂锚杆在架空人车安装中的应用

东曲矿全煤巷道中安装架空人车无法采用常规的预埋梁法固定托轮横梁，根据锚杆工作原理，将树脂锚杆应用到架空人车的托轮横梁固定安装中，较好地解决了架空人车在全煤巷道中的安装难题，达到了预期的安装效果。     

《数学模型》体系探讨与竞赛训练

本文就数学模型作为一门独立的教学课程，对其理论体系进行了探讨，并提出了详细的体系设计。     

群自同构全提升本盖结构

根据Ｆｒｙ的群与Ｇｒｕｅｎｂｅｒｇ的扩张范畴（Ｔｒ     

四川省邛崃市土鸭寄生虫病初步调查

临床剖检土鸭20只，通过体外及体内寄生虫检查，发现体外感染为阴性，体内寄生虫病中吸虫感染率较高，其中纤细背孔吸虫最高(45％)，感染强度大(3～57条)。并发现食道粘膜的毛细线虫卵感染率甚高(65％)，针对这些情况进行了分析，认为与本地的自然环境有着密切关系，从而为养殖户防治疾病提供依据。