Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2

Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4-phorbol 12-myristate, 13-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4-phorbol 12, 13 didecanoate was ineffective.These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.     

Pharmacodynamic profile of CQP 201-403, a novel 8α-amino-ergoline

Summary The profile of action in animals of CQP 201-403, a novel 8-amino-ergoline, is in most aspects that of a very potent dopaminomimetic, both as a prolactin secretion inhibitor, and at the levels of the CNS and the cardiovascular system. Qualitatively CQP 201-403 differs slightly from bromocriptine and apomorphine in its effects on the CNS (no influence on serotonin metabolism in the rat cortex; induction of masculine mounting behavior in rats) and the cardiovascular system of the dog (reflex tachycardia in response to a blood-pressure fall). In man the new compound proved to be highly active in lowering prolactin serum levels and to be more potent than bromocriptine (Parlodel^®).In memory of Dr Annemarie Closse, who died 14 June 1987.     

Flow-induced release of adenosine 5′-triphosphate from endothelial cells of the rat mesenteric arterial bed

Adenosine 5-triphosphate (ATP) was released into the perfusate of rat isolated mesenteric arterial beds during each of two consecutive increases in flow. There was no significant difference between the amounts of ATP released on each occasion. Substance P was also released into the perfusate by increased flow, although its release was more variable. Removal of the endothelium of the mesenteric vessels with sodium deoxycholate led to a significant reduction (74%) in the amount of ATP released compared with the release before the endothelium had been removed. This suggests that the ATP released into the mesenteric arterial perfusate during increased flow arises from endothelial cells.     

Determination of erythrocyte pyrimidine 5′-nucleotidase activity by31P nuclear magnetic resonance: Comparison of normal controls and multiple sclerosis patients

Summary A technique to assay erythrocyte pyrimidine 5-nucleotidase activity in situ using³¹P nuclear magnetic resonance spectroscopy is presented. The assay is chemically specific, simple and applicable to untreated lysates. A comparison of enzyme levels in normal controls and in multiple sclerosis patients employing the assay yielded no significant differences between both groups. Difficulties encountered in the quantitative analysis of the assay using¹H-NMR spectroscopy are briefly discussed.     

Endorcine cells producing regulatory peptides

Summary Recent data on the immunologication of regulatory peptides and related propeptide sequences in endocrine cells and tumours of the gastrointestinal tract pancreas, lung, thyroid, pituitary (ACTH and opioids), adrenals and paraganglia have been revised and discussed. Gastrin, xenopsin, cholecystokinin (CCK), somatostatin, motilin, secretin, GIP (gastric inhibitory beenrevised and discussed. Gastrin, xenopsin, cholecystokinin (CCK), somatostatin, motilin, secretin, GIP (gastric inhibitory polypeptide), neurotensin, glicentin/glucagon-37 and PYY (peptide tyrosine tyrosine) are the main products of gastrointestinal endocrine cells; glucagon, CRF (corticotropin releasing factor), somatostatin, PP (pancreatic polypeptide) and GRF (growth hormone releasing factor), in addition to insulin, are produced in pancreatic islet cells; bombesin-related peptidesare the main markers of pulmonary endocrine cells; calcitonin and CGRP (calcitonin gene-related peptide) occur in thyroid and extrathyroid C cells; ACTH and endorphins in anterior and intermediate lobe pituitary cells, -MSH and CLIP (corticotropoin-like intermediate lobe peptide) in intermediate lobe cells; met- and leu-enkephalins and related peptides in adrenal medullary and paraganglionic cells as well as in some gut (enterochromaffin) cells; NPY (neuropeptide Y) in adrenalin-type adrenal medullary cells, etc.. Both tissue-appropriate and tissue-inappropriate regulatory peptides are produced by endocrine tumours, with inappropriate peptides mostly produced by malignant tumours.     

泵闸混凝土施工期智能温控关键信息识别

为实现施工期大体积混凝土温控要素的智能快速识别,提高智能温控系统的反馈及预测模型精度,提出了一套智能算法对物联网技术采集到的各类温控要素原始测值进行识别及转化。针对浇筑温度、浇筑时间、最高温度、内外温差、冷却通水起止时间与表面保温覆盖等施工期关键温控参数,结合工程经验分别给出相应识别任务的判定逻辑并编写对应程序,然后应用多个实际工程数据进行验证,并分析各识别功能的准确率。验证结果表明,本文算法识别效果良好,能够实现温控要素采集的自动化、智能化、快速化、精准化,具有较强的工程实用价值。     

小型化折叠型RFID抗金属标签天线的设计

设计一种小型化折叠型超高频射频识别标签天线.天线由开槽的辐射贴片、 4个感性短接面和两片金属接地板组成.通过改变短接面尺寸及右侧短接面与接地面的距离,可以有效地调谐标签天线的谐振频率及其阻抗,总尺寸(L×W×h)为40 mm×40 mm×1.5 mm.实验结果表明：标签天线阻抗匹配良好,|S₁₁|＜﹣10dB带宽为880～930 MHz.将标签天线放置于200 mm×200 mm的金属板上,4 W有效全向辐射功率条件下的最大实测阅读距离可达到11.2 m.该标签天线通过适当的开槽实现小型化,同时具有抗金属、识别距离远等优点,可较好地应用于工业物联网相关测量领域中.     

Hammerstein系统遗忘因子有限窗口分解辨识

提出一种带遗忘因子和分解辨识策略的有限数据窗口递归最小二乘Hammerstein系统辨识方法。针对Hammerstein系统具有耦合参数的问题,将Hammerstein系统分解为2个子系统：一个子系统包含线性子系统参数,另一个子系统包含非线性子系统参数;提出一种基于遗忘因子的有限窗口递归最小二乘方法对分解模型进行在线递归估计;仿真示例验证了所提算法能够快速跟踪参数,实现对Hammerstein系统的精确辨识。