马铃薯病毒一步法RT-PCR诊断研究

运用一步法RT-PCR对马铃薯和烟草中的马铃薯X病毒、马铃薯Y病毒和马铃薯卷叶病毒进行了检测,实验表明:对酚-SDS方法提取的病毒核酸进行扩增,一步法RT-PCR检测到的扩增产物的灵敏度较两步法RT-PCR的高100倍左右.两种提取方法对比,用一步法RT-PCR进行扩增,检测到的PEG法扩增产物的灵敏度较酚-SDS法扩增产物的灵敏度高3倍.建立了快速、简便、灵敏度高、特异性强的马铃薯病毒一步法RT-PCR检测技术.     

老年性痴呆症患者的EEG近似熵特征初探

老年性痴呆症是一种后天获得性中枢神经系统退行性疾病，严重地危害着人们的身体健康和生活质量，为了探索老年性痴呆症脑电图中隐含的信息，对老年性痴呆症患者和健康对照者的脑电图（EEG）近似熵进行分析，并绘制其脑电脑电信息图，对照健康人，发现患者EEG近似熵普遍下降，尤以右额叶，颞叶区显著，提示EEG近似熵与大脑功能状况有一定联系，在一定程度上可以反映大脑功能状态。     

运营公路路基病害快速无损综合检测新技术

针对现有路基病害无损检测方法检测精度低、速度慢、对交通影响大以及很难在运营公路上进行路基病害检测等问题,本研究从探地雷达、高密度电法及瑞雷波法检测原理出发,对上述3种检测设备进行改造,设计了路基病害综合处理平台,提出了运营公路路基病害快速无损综合检测新技术．研究结果表明,采用空气耦合天线探地雷达,并对高密度电法电极及瑞雷波法检波器布设方法进行改造,可实现公路路基病害快速检测;以不同检测方法检测指标及检测对象互补性为基础,采用多传感器融合的数据采集和处理方法,可实现路基病害综合检测;通过路基病害综合处理平台,可实现路基病害检测数据的联合解译及路基病害的可视化及网格化显示．运营公路路基病害快速无损综合检测新技术的应用,可以提高路基病害检测精度与效率,大大促进路基检测与养护水平的发展．     

关于民族遗传素质前景的探讨

本文从遗传模型出发,针对我国生有遗传病患儿允许生第二胎的政策,仅以AR遗传病为例,揭示了导致人口遗传素质逐代下降的发展趋势,同时,泛论了影响优生的其他因素。     

臭氧水对土传病原真菌的杀灭作用

为探寻土壤绿色消毒方法,研究不同质量浓度的臭氧水对3种土传病原真菌尖孢镰刀菌（Fusarium oxysporum）、立枯丝核菌（Rhizoctonia solani）及灰霉菌（Botrytis cinerea）的杀灭效果。结果表明：质量浓度1.0~5.0 mg/L的臭氧水对3种病原真菌的孢子都有一定的杀灭作用,臭氧水质量浓度是影响杀灭率的主要因素,作用时间对杀灭率无显著影响;提高臭氧水质量浓度及增加浇灌次数能显著增强对土壤中3种病原真菌的杀灭率;臭氧水浇灌土壤能显著降低植株的发病率,但植株的生长受到抑制,添加木霉能消除这种抑制作用。     

禽网状内皮组织增生流行病学概述

禽网状内皮组织增生病病毒属于C型反转录病毒。该病毒可引起家禽免疫功能抑制和慢性肿瘤。本文就禽网状内皮组织增生病病毒的生物学特性、引起免疫抑制机制、引起肿瘤的发生和预防措施的研究进行简要综述。     

Application of EEG multipole model in the diagnosis of Alzheimer''''s disease

In order to find the electroencephalogram （EEG） characteristic parameters typical of Alzheimer＇s disease （AD） and explore an effective diagnostic method, a new mobile current multipole model was proposed to simulate the AD patient＇s cortical dipole source activities. The indicators of goodness of fit （GOF） and DtononD （DD） were calculated from EEG samples to evaluate the performance of this model. Relevant results showed that this multipole model with higher GOFvalues and larger DD change well fitted the pathological electrical activities of cortical neurons aroused by AD＇s extended sulcus and gyrus in the cerebral cortex. Meanwhile, the products of DD mean ＆ standard variance were found in a clear linear correlation with the diagnostic data of mini-mental state examination （MMSE） used in AD clinics. Furthermore, by tracing this multipole model＇s indicators in typical patients and contrasting with the functional magnetic resonance image （fMRI） as AD progressed, we suggested that the DD index may be suitable for monitoring the AD developments as a new diagnostic parameter.     

基于AP算法支持向量机的设计与应用

设计一种基于AP聚类算法和SVM分类器相融合的新的混合分类器, 使用AP聚类算法优化数据集, 得到了高质量、 小样本的SVM分类器训练集. 实验结果表明: 与传统的SVM分类器相比, 混合分类器具有更高的分类精度; 在心脏病预测上, 该分类器的效果较好.     

实验性兔出血症病理形态学动态观察

本试验应用光镜、电镜技术,对人工感染兔出血症病毒(RHDV)的成年兔、30～40日龄幼兔以及自然感染死亡病例的各种组织器官进行了病理形态学动态观察。结果表明,成年兔对本病毒易感,表现出与自然病例相似的典型变化,而30～40日龄幼兔则易感性低,缺乏特征病变。在患兔的肝细胞中观察到病毒粒子呈散在或聚集成包涵体样存在于核内及胞浆中,佐证本病毒为DNA病毒。     

RNA interference-mediated inhibition of Hepatitis B Virus replication

Persistent and recurrent infection of hepatitis B virus (HBV) represents one of the most common and severe viral infections of humans, and has caused a formidable health problem in the affected countries. Currently used antiviral drugs have a very limited success on controlling HBV replication and infection. RNA interference (RNAi), a process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of target mRNA in mammalian and plant cells, has recently been used to knockdown gene expression in various species. In this study, we sought to determine whether RNAi-mediated silencing of HBV viral gene expression could lead to the effective inhibition of HBV replication. We first developed RNAi vectors that expressed small interfering RNA (siRNA) and targeted the HBV core or surface gene sequence. Our results demonstrated that these specific siRNAs efficiently reduced the levels of corresponding viral RNAs and proteins, and thus suppressed viral replication. Treatment with siRNA gave the greatest reduction in the levels of HBsAg (92%) and in HBeAg (85%) respectively in the cultured cell medium. Our findings further demonstrated that the RNAi-mediated antiviral effect was sequence-specific and dose-dependent. Therefore, our findings strongly suggest that RNAi-mediated silencing of HBV viral genes could effectively inhibit the replication of HBV, hence RNAi-based strategy should be further explored as a more efficacious antiviral therapy of HBV infection.