Protective effect of Shigyaku-to,a traditional Chinese herbal medicine,on the infection of herpes simplex virus type 1 (HSV-1) in mice

The antiviral activity of Shigyaku-to (TJS-109), a traditional Chinese herbal medicine, was investigated in mice infected with herpes simplex virus type 1 (HSV-1). TJS-109 is a combination of the medicinal plant extracts fromZingiberis siccatum rhizoma,Aconiti tuber andGlycyrrhizae radix in a specific proportion. Mice infected with a 10 LD₅₀ dose of HSV-1 were treated with TJS-109 orally at doses of 1.25 to 20 mg/kg 2 days before, and 1 and 4 days after the infection. The treated groups had 80% (1.25 mg/kg), 40% (5 mg/kg) and 23% (20 mg/kg) mortality rates 25 days after the infection as compared with a 100% mortality rate in control mice treated with saline. When HSV-1 infected mice (recipients) received CD8⁺T cell fractions derived from spleens of mice treated with TJS-109 (donors), 70% of recipients survived, as compared with 0% survivors in the groups of mice treated with saline, B cell fractions, CD4⁺ T cell fractions or macrophage-enriched fractions prepared from the same donors. TJS-109 did not show any virucidal activities against HSV-1 or any virostatic activities on the growth of HSV-1 in Vero cells. These results suggest that TJS-109 protected mice exposed to lethal amounts of HSV-1 through the activation of CD8⁺ T cells.     

某鸡场鸡传染性法氏囊病弱毒疫苗饮水免疫后发生的一次鸡病的诊断

１９９１年４月上旬，四川某鸡场对３５７０只１３日龄依莎雏鸡作法氏囊病弱毒疫苗饮水免疫，１４日龄时发病死亡９只，到２３日龄９天内共死亡８１４只，病死率达２２．８％。对２０只送检病死鸡的沙门氏菌分离率为１／２０株（５％）．但病料人工感染小白鼠试验为阴性，证明非病原菌所致，用病料与新城疫血清（抑制价１：６４０）作用后接种非免疫健康鸡１组的存活率为７５％，直接接种未与新城疫血清作用病料的２组存活率为５０％，剖检回归本动物试验自然死亡和人为处死的病鸡发现了较为典型的法氏囊病变，由此认为：法氏囊弱毒疫苗饮水免疫是引起鸡群发病死亡的主要原因。     

一类含时滞非线性传染病模型的渐近分析

本文研究了一大类具有潜伏期的传染病模型,其中潜伏期可长可短,并对模型进行无量钢化,归结为一个含小参数的方程组,运用奇摄动的边层函数法进行了渐进分析,求出了一致有效的渐进解。     

茶刺蛾[Darna trima(more)]颗粒体病毒鉴定及核酸与蛋白质特性的初步研究

1985年从四川珙县分离到的茶刺蛾病原物,经感染试验,组织病理研究,包涵体及病毒粒子超微结构研究,核酸类型鉴别等,定名该病原物为茶刺蛾颗粒体病毒.另对该病毒核酸作了限制性内切酶分析和热变性试验,测得该病毒核酸为双链DNA分子,分子量为64.11×10~6d,93.87kb,Tm值为67.3℃,(G+C)含量为32.7％,包涵体蛋白的氨基酸组分中Asp、Glu和Arg含量高,占氨基酸总量的34.12％,His,Cys和Met含量很少。该病毒对茶刺蛾幼虫有很强的感染力,氨基酸在生物防治上有潜在应用前景.     

血清唾液酸检测癌症和良性疾病研究

对癌症、良性肿瘤、常见病患者及健康者进行血清唾液酸（ＳＡ）检测，并对确诊或尚未确诊病例进行动态监测，结果ＳＡ正常参考值为２．０７±０．４７ｍｍｏｌ／Ｌ，性别之间无显著差别（Ｐ＜０．５）癌症组、良性疾病组、健康组之间ＳＡ依次有极显著性差别（Ｐ＜０．００１），应用ＬＲ和ＰＴＬ等指标对ＳＡ在临床应用进行系统评价，指出ＳＡ检测癌症特异性较差。且报道肾炎，尤其是急性肾炎和恙虫病ＳＡ也会显著升高，过度饮酒和抽烟也会升高，ＳＡ作为检测癌症必须动态监测，作为检测有否疾病是一种较好的试验。     

中华鳖水泡病病原病理学研究

１９９３年８月信阳师院生物系特种动物养殖研究所的试验鳖场孵化出一批小鳖。同年１０月有一只鳖得了一种新的疾病，病鳖颈根部和右下肢基部各有一大水泡，暂名“水泡病”。本文对其分离菌进行了研究，鉴定为嗜水气单胞菌。中华鳖水泡病的致病机理，可能与病原菌的毒力与传染途径有关，而鳖的抗病能力是能否引起该病的关键，外界环境因素是引起该病的诱因。     

青年颈椎病初探

长期以来,人们普遍认为颈椎病为中老年人的多发病,但在我院自1984年7月至1987年12月止的355份颈椎病病例中,年龄在30周岁以下者竟有43例,比率高达12.11%.由于医师、患者均对青年罹患颈椎病之可能缺乏认识,其中13例(30.23%)曾被误诊,29例(67.44%)患者自恃年青,拖延到症状出现,1—9年以后才来就医.本文所强调的是对颈椎病的注意力不应仅限于中老年人,无论是临床或基础研究,都应将视野扩展到青年领域中去.并对青年颈椎病的临床表现、病因、发病机理及治疗等问题进行了初步探讨.     

鸡新城疫免疫程序和免疫监测在生产中的应用

在44群共35万只鸡中,应用Ⅰ抗体监测以制定合理的免疫程序。对于集约化养鸡场,第一次在12日令时用Ⅱ系苗作饮水免疫;第二次在40——50日令用Ⅱ系苗作气雾免疫,然后在110——120日令时再用Ⅰ系苗作气雾免疫一次、可使鸡只的HI抗体平达到2~3以上。     

Peptide aptamers: exchange of the thioredoxin-A scaffold by alternative platform proteins and its influence on target protein binding

Peptide aptamers have emerged as powerful new tools for molecular medicine. They can specifically bind to and functionally inactivate a given target molecule under intracellular conditions. Typically, peptide aptamers are generated by screening a randomized peptide expression library, displayed from the Escherichia coli thioredoxin A (TrxA) protein. Here, we transferred peptide moieties from defined TrxA-based peptide aptamers to alternative scaffold proteins, such as the green fluorescent protein and staphylococcal nuclease. Yeast and mammalian two-hybrid assays as well as in vitro binding analyses show that the TrxA scaffold can be a major determinant for the binding of peptide aptamers. In addition, we demonstrate that TrxA can correctly display peptide sequences that correspond to the binding domains of natural interaction partners. Therefore, sequence analyses of TrxA-based peptide aptamers, isolated by two-hybrid screening from randomized expression libraries, should also be useful to find cellular binding partners for a given target protein, by homology. Received 1 August 2002; received after revision 17 September 2002; accepted 19 September 2002 RID="*" ID="*"Corresponding author.