西藏网翅蝗科两新种（直翅目：蝗总科）

记述采自西藏贡嘎地区网翅蝗科两新种，即贡嘎牧草蝗Ｏｍｏｃｅｓｔｕｓ ｇｏｎｇｇａｒｅｎｓｉｓｓｐ．ｎｏｖ。及贡嘎缺耳蝗Ａｔｙｍｐａｎｕｍ ｇｏｎｇｇａｒｅｎｓｉｓ ｓｐ．ｎｏｖ。     

热休克对粗糙脉孢菌核酸内外切酶的影响

将快速生长的粗糙脉孢菌Ｎｅｕｒｏｓｐｏｒａｃｒａｓｓａ培养液从３０℃升温至４５℃，热休克处理２小时，７５％有活性的核酸内外切酶ｅｎｄｏ－ｅｘｏｎｕｌｅａｓｅ（此酶为ＤＮＡ修复酶）从核及液泡中释放到胞质并以无活性的形式储存起来：用放线菌酮处理，不能阻止此酶的释放，但能使灭活降低；致使胞质的酶活性提高５－１５倍。热休克菌体核酸内外切酶的灭活是由于某种抑制物质的作用，后者来自酶前体的自身降解。作者推测由热休克诱导了一种蛋白酶，此酶使核酸内外切酶前体水解为抑制物质。     

广西舞虻科三新种记述——双翅目:短角亚目

双翅目(Diptera)短角亚目(Brachycera)的舞虻科(Empididae)昆虫大多为捕食性。成虫或幼虫常为农林害虫的有效天敌,在生物防治上有潜在的前途,广西的舞虻区系近年经杨集昆与杨定研究(1986、1991)已报道有3属15种,本文再增加3新种和广西新记录的2属,新种的模式标本保存在北京农业大学昆虫标本室。     

面对"新公共管理"运动的我国行政管理改革

毫无疑问,“新公共管理”运动是西方国家行政管理领域的创新,并对我国的行政管理改革产生了启示作用。但由于我国的行政管理改革有着与西方“新公共管理”运动不同的问题和任务,因此,我们不能原样复制西方的“新公共管理”模式。我们应当结合中国的实际情况,消化和整合西方“新公共管理”的思想。     

论"新红学"的知识谱系

“新红学”的基本构成是“实证与实录合一”．胡适以“科学精神”演述乾嘉学术方法,以“自然主义”、“自叙传”去演述传统的史学实录观念．实证研究体现一种科学精神,实录观念则是一种反科学的观念．     

电影发展运动及其影响

介绍了在电影艺术发展史上产生重大影响的4次主要电影运动:欧洲先锋派电影运动、意大利新现实主义电影运动、法国新浪潮电影运动和德国新电影运动,认为它们对于当代电影朝着多元化、全方位的发展起了决定性的作用。     

RNA-dependent RNA polymerase encoded by hepatitis C virus: biomedical applications

The hepatitis C viruses (HCVs) are a group of small enveloped RNA viruses that have been viewed as a leading cause of chronic hepatitis in humans. Infections by HCV represent a serious global health problem, because millions of people worldwide are infected and no efficient treatment is available at the present time. Since HCV was identified in 1989, considerable effort has been devoted to the discovery and development of novel molecules to treat HCV-related diseases. One of the approaches is the development of novel inhibitors that interrupt the normal functions of HCV NS5B, an RNA-dependent RNA polymerase essential to HCV replication. This review summarizes recent advances in the biochemical and structural understanding of HCV NS5B polymerase as well as in the development of antiviral agents targeting this important enzyme. Received 19 March 2002; received after revision 23 April 2002; accepted 23 April 2002     

新经济成因、特点和对策初探

新经济就是由于计算机和“网络”等高新技术的应用,推动了经济高速增长和持续发展的经济。中国走新经济道路,既不能照抄美国的经验,也不能完全否认美国的那一套。总之要以邓小平建设有中国特色社会主义理论为指针,走新经济之路。     

Recombinant expression of perchloric acid-soluble protein reduces cell proliferation

Perchloric acid-soluble protein (PSP) may play an important role in the regulation of cellular physiological functions because it has been highly conserved throughout evolution; however, this role has not been well elucidated. In previous reports, we suggested that PSP regulates cell proliferation. In this study, we examined the effect of PSP expression on proliferation of the normal rat kidney cell line NRK-52E, the rat hepatocyte cell line RLN-10, and the rat hepatoma cell line dRLh-84. Cells transfected with pcDNA-sense-PSP (pcDNA-S-PSP) over-expressed PSP mRNA and protein, and cell proliferation of the transfected cells was suppressed compared with that of cells transfected with pcDNA-empty (pcDNA-E). Cell viability of pcDNA-S-PSP-transfected cells was similar to that of pcDNA-E-transfected cells. Thus, over-expression of PSP suppresses cell proliferation without any influence on cell viability. These findings are the first to report an inhibitory activity of PSP on cell proliferation. Received 27 April 2001; received after revision 8 June 2001; accepted 8 June 2001