用脾内免疫法制备抗人尿激酶单克隆抗体

用常规免疫法制备抗人尿激酶单克隆抗体费时、费样品。本文采用脾内直接注射的免疫方法,利用杂交瘤技术制备了3C_6抗尿激酶单克隆抗体,3C_6单克隆抗体属IgM亚类,主要识别MW 54,000的高分子量尿激酶和MW 33,000的低分子量尿激酶。3C_6单克隆抗体与结构类似的胰蛋白酶和纤溶酶原无交叉反应,与胰凝乳蛋白酶原和胰凝乳蛋白酶有一定程度的交叉反应。     

中华眼镜蛇毒神经生长因子的研究

采用ＣＭ－ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ和ＳｅｐｈｏｓｉｌＣ８以及ＨＰＬＣ层析方法，自广西产中华眼镜蛇毒中分离纯化神经生长因子（ＮＧＦ），此ＮＧＦ经８ｄ鸡胚背根神经节体外培养证明具有促进神经纤维生长的活性。经ＨＰＬＣ及聚丙烯酰胺凝胶电泳鉴定为单一组成，经ＳＤＳＰＡＧＥ及ＨＰＬＣ测定分子量分别为２４．１ＫＤ和２４．９ＫＤ，等电聚焦电泳测得ｐＩ为８．２，氨基酸组分分析表明ＮＧＦ含酸性氨基酸较少，通过１２５｜标记ＮＧＦ测定出ＮＧＦ在生物体内主要分布于肾、肺、及周围神经     

抗人Connexin31单克隆抗体的获得与应用

间隙连接蛋白31(Connexin31,Cx31)是间隙连接蛋白(Connexin)家族的一员,目前对于Cx31的功能及其调节方式知之甚少。本实验利用多肽合成的方法合成Cx31羧基端一个多肽片段(250-266AA),经HPLC纯化后偶联到匙孔槭血蓝蛋白,免疫Balb/c小鼠、采血检测产生阳性抗体、脾细胞与骨髓瘤细胞SP2/0融合、筛选阳性克隆建株、分泌的抗体经Westernblotting、细胞免疫荧光染色证实得到的抗体为抗间隙连接蛋白31的特异抗体。     

小鼠血清甲状腺激素水平与免疫功能的衰老变化

利用放射免疫测定法和免疫功能指标的检测方法研究了 2 0月龄老年小鼠血清甲状腺激素水平和免疫功能的衰老变化 ,并用外源性甲状腺激素胃内给药的方法探讨了甲状腺机能和免疫功能变化间的因果关系 .结果证明 ,老年期甲状腺机能和免疫功能都有明显的衰退变化 ,且二者间存在有明显的因果关系 .甲状腺机能衰退导致免疫功能紊乱可能是机体衰老的基本过程之一     

Piezoelectric immunoassay of thyroidal triiodothyronine

A re-utilizable piezoelectric crystal (PC) immunosensor for thyroidal triiodothyronine (T3) has been developed. The crystal was coated with protein A and then reacted with T3 antibody. T3 could be detected in the range of 0.5×10⁻⁶ g/L to 10.0×10⁻⁶g/L by the, system. Crystal was regenerated by saturating with T3 and subsequent binding of a new anti-T3 antibody layer. The T3 assay could be repeated up to 5 times using each crystal. Supported by the National Natural Science Foundation of China Zha Hongying: born May, 1969, Ph. D.     

抗人凝血VIII因子的单克隆抗体制备及其鉴定

经凝胶过滤和亲和层析分离得到的人抗血友病A因子——VIII因子抗原VIII:CAg,在用一期法和火箭免疫电泳证实其纯度后,免疫Balb/c小鼠和包被免疫测定板。采用常规鼠杂交瘤技术经间接ELISA法筛选、克隆化得到四个稳定分泌抗VIII:C的单克隆抗体的细胞株,即DC8,DE4,DE7和DE8,腹水效价为1:1×10~4～1:1×10~5,一期法抑制反应表明,单克隆抗体对VIII:C活性有显著的抑制作用,但其抑制是不完全的,对抗体进行了纯化及分析,并将单抗用于血友病A多态性的分析。     

Alzheimer's disease: fundamental and therapeutic aspects

Alzheimer's disease is the most common type of progressive and debilitating dementia affecting aged people. In some early — as well as late-onset familial cases, a genetic linkage with chromosomes 14, 21 (early-onset) or 19 (late-onset) has been indicated. Furthermore, a direct or indirect role has been attributed to normal or structurally altered amyloid -protein (concentrated in senile plaques) and/or excessively phosphorylated tau protein (located in neurofibrillary tangles). Degeneration of cholinergic neurons and concomitant impairment of cortical and hippocampal neurotransmission lead to cognitive and memory deficits. Several compounds are being tested in attempts to prevent and/or cure Alzheimer's disease, including tacrine, which has very modest efficacy in a sub-group of patients, and new acetylcholinesterase inhibitors. Pilot experiments have also been launched using nerve growth factor (NGF) to prevent or stabilize the processes of cholinergic pathway degeneration. Alternatively, antioxidants, free radical scavengers and/or non steroidal anti-inflammatory agents may be screened as potential therapies for neurodegenerative diseases induced by multiple endogenous and/or exogenous factors. The recent use of transgenic mice, in parallel with other genetic, biochemical and neurobiological systems, in vivo and/or in vitro (cell cultures), should accelerate the discovery and development of specific drugs for the treatment of Alzheimer's disease.     

乙肝疫苗预防接种的临床观察分析

本文对新入校大学生及全校部分教职工及子女共985例进行了计划免疫及预防接种，接种后抗体阳转者857例，阳性率为87％，结果表明：此方法为预防乙型肝炎的最理想方法。