Antigenic epitope peptides of influenza H3N2 virus neuraminidase gene based on experiments

The neuraminidase (NA) in viral surface is one of the main subtype-specific antigen of influenza type A viruses.Neuraminidase is an enzyme to break the bonds between hemagglutinin (HA) and sialic acid to release newly formed viruses from infected cells.In this study,the H3N2 subtype virus NA genes were sequenced and NA proteins were screened for B-cell epitopes and assessed based on immunoinformatics.Based on this results,four peptides DR6,EY7,VG8 and RE8 (covering amino acid residues 151-156,368-374,398-405 and 428-435,respectively) of the NA protein were synthesized artificially.These peptides were used to immunize New Zealand rabbits subcutaneously to raise antisera.Experimental results showed that these four peptides were capable of eliciting antibodies against H3N2 viruses in a specific and sensitive feature,detected in vitro by enzyme-linked immunosorbent assay.Moreover,hemadsorption anti-releasing effects took place in three three-antisera-mixtures at a dilution of 1:40.Alignment using NA gene database showed that amino acid residues in these four epitope peptides were substituted at specific sites in all the NAs sequenced in this study.It was suggested that these NA epitope peptides might be used in combination with HA proteins as vaccine antigens.     

NA样本下分布函数估计的联合渐近分布

研究了NA样本下分布函数的核估计在有限个不同点上的联合渐近分布,证明了该分布为多维正态分布,从而将分布函数的核估计从单点推广到多点,扩大了分布函数核估计的应用范围.     

NA随机变量之和的大偏差与指数估计

对ＮＡ随机变量序列建立了类似于独立随机变量序列的大偏差概率不等式与指数估计。     

随机变量部分和之和的L~r收敛性

利用截尾和矩不等式方法,研究在剩余Cesàroα可积条件下NA序列部分和之和的Lr(1≤r<2)收敛性和φ混合序列部分和之和的Lr(r>2)收敛性,推广和改进了一些已有的结果.     

Strong stability of generalized Jamison’s weighted sums of NA sequences

The a. s. convergence of NA series as the point of departure is studied.     

NA随变量序列的Fuc—Nagaev型不等式

本文给出ＮＡ随机变量序列的一些概率不等式,进一步推广了文「１」「２」中的一些结果。     

NA样本下变方差模型估计的强相合性

考虑变方差回归模型Yi=g(ti) σiei,i=1,2 ,… ,n ,其中σ2 i=f(ui) ,(ti,ui)为非随机设计点列 ,g(·)和f(·)均为未知函数 .当随机误差ei 为NA变量时 ,讨论了 g(t)的一般加权估计 g^n(t)的一致强相合性 ,以及f(x)的 一般加权估计 f^n(u)的强相合性和一致强相合性     

行为NA的随机变量三角阵列的完全收敛性

利用NA序列的一个矩不等式,研究了行为NA的随机变量三角阵列在Cesaro意义下被随机变量X控制下的完全收敛性,推广了行独立随机变量三角阵列相应的结果.     

非同分布NA列滑动平均过程的完全收敛性

根据Li等(1992)建立的i.i.d.随机变量序列滑动平均过程的若干极限结果,建立关于非同分布NA列的滑动平均过程的完全收敛性.