Enhancement of monacolin K production via intergeneric protoplast fusion between Aspergillus terreus and Monascus anka

Intergenric protoplast fusion between Aspergillus terreus CA99 and Monascus anka M-3, the high and low producers of monacolin K respectively，was performed for enhancement of monacolin K production. The 24-hour-old mycelia of A. terreus CA99 and M. anka M-3 were treated with 0.5% lywallzyme, 0.3% snailase and 0.3% cellulase at 34℃ for 5 h and at 30℃ for 3.5 h, and their protoplasts formation reached 1.76×10⁷/mL and 1.68×10⁷/mL respectively. Parental protoplasts were irradiated with a 30 W UV light away from 30 cm for 3 min and then mixed. The mixture was incubated with 30% PEG 6000 for 15 min. The reviving fusants were isolated on the regeneration plates. Of the 363 fusants isolated, over 100 showed enhanced monacolin K production compared with the parental strain M. anka M-3. Ten of them produced monacolin K about 1.6-fold of that M.anka M-3 does and the monacolin K titer of two fusants (F49 and F104) increased by about 1-fold. The monacolin K yields of F49 and F104 were 460 μg/mL and 457 μg/mL respectively. In optimized fermentation medium, the monacolin K titer of F49 reached 1216 μg/mL.     

基于模糊推理的小波域图像融合规则

现有的2种主要的小波域融合规则,即选择绝对值最大系数规则和选择与加权平均相结合的规则,都存在着融合信息不完整和易受噪声干扰等缺点.为克服这些缺点,使融合图像包含尽可能多的源图信息,提出了基于模糊推理的小波域融合规则.此规则根据源图像小波系数的局部区域特征,通过模糊推理确定各源图像相应系数的权值,再对相应系数加权平均得到融合后的小波系数.实验结果表明,使用基于模糊推理的融合规则融合的图像,在峰值信噪比以及与源图像之间的互信息量等方面均优于使用2种现有规则进行融合所得的图像.新规则有效地克服了现有规则的缺点,具有更好的信息完备性和鲁棒性,尤其在源图像受到噪声污染的情况下,其优势更加明显.     

光子晶体光纤熔接机理的研究

光子晶体光纤（PCF,Photonic Cpystal Fiber）的熔接技术为PCF产品的应用和开发提供了条件。本文主要介绍了影响PCF熔接的主要因素，比较了传统电弧熔接方法和激光熔接方法的优缺点，阐述了激光熔接的基本原理和工作流程，为PCF激光熔接机的制作打下基础。     

基于Spline金字塔的图像融合方法

图像融合作为信息融合的一个重要领域已广泛应用于遥感、医学、计算机视觉、军事目标探测和识别等多方面。文章在传统的多分辨金字塔结构—Laplace金字塔的基础上提出Spline金字塔融合算法,有一定的探索性和创新性。在融合过程中着重讨论了塔形结构的分解和重构及融合规则。由实验结果可以判断Spline金字塔算法是一种有效的图像融合算法。     

基于最小二乘支持向量机和证据理论的交通数据融合

针对基于浮动车辆数据(floating car data,FCD)的城市道路交通信息采集系统存在的问题,提出一种基于最小二乘支持向量机(LS-SVM)和证据理论的数据融合方法,通过融合地感线圈采集的交通流量信息,提高FCD系统交通速度信息采集的准确性.利用LS-SVM回归得到速度-流量关系曲线的临界速度参数,再根据历史数据库用统计方法计算出流量-速度关联规则的可信度矩阵,在得到这些经验知识的基础上,定义了两种证据源的基本概率分配函数.最后,通过D-S证据理论对两种证据源进行数据融合,获得融合后的速度信息.实地跑车实验结果论证了融合算法的有效性和可靠性.     

Data fusion based on fuzzy measures

Choquet integral based on fuzzy measure is a very popular data fusion approach. A major problem in applying the Choquet integral is how to determine a large number of fuzzy measures as the number of attributes increases. The λ-fuzzy measure proposed by Sugeno is a powerful method to resolve this problem. However, the modeling ability of the λ-fuzzy measure is too limited to satisfy actual requirements. In this paper, an extended λ-fuzzy measure is proposed using Shapley value index, and the limitation of the λ-fuzzy measure is significantly overcome under little additional computational loads. The extended fuzzy measure has stronger modeling power than the λ-fuzzy measure, straightforwardly representing interaction among attributes. We apply the extended fuzzy measure to an artificial data set and a real dataset in an iron-steel plant. The results verify the usefulness of the extended fuzzy measure compared with other main existing methods.     

Data fusion based on fuzzy measures

Choquet integral based on fuzzy measure is a very popular data fusion approach.A major problem in applying the Cho- quet integral is how to determine a large number of fuzzy measures as the number of attributes increases.Theλ-fuzzy measure proposed by Sngeno is a powerful method to resolve this problem.However,the modeling ability of theλ-fuzzy measure is too limited to satisfy actual requirements.In this paper,an extendedλ-fuzzy measure is proposed using Shapley value index,and the limitation of theλ-fuzzy measure is significantly overcome under little additional computational loads.The extended fuzzy measure has stronger modeling power than theλ- fuzzy measure,straightforwardly representing interaction among attributes.We apply the extended fuzzy measure to an artificial data set and a real dataset in an iron-steel plant.The results verify the usefulness of the extended fuzzy measure compared with other main existing methods.     

Enhancement of monacolin K production via intergeneric protoplast fusion between Aspergillus terreus and Monascus anka

Intergenric protoplast fusion between Aspergillus terreus CA99 and Monascus anka M-3, the high and low producers of monacolin K respectively,was performed for enhancement of monacolin K production. The 24-hour-old mycelia of A. terreus CA99 and M. anka M-3 were treated with 0.5% lywallzyme, 0.3% snailase and 0.3% cellulase at 34℃ for 5 h and at 30℃ for 3.5 h, and their protoplasts formation reached 1.76×107/mL and 1.68×107/mL respectively. Parental protoplasts were irradiated with a 30 W UV light away from 30 cm for 3 min and then mixed. The mixture was incubated with 30% PEG 6000 for 15 min. The reviving fusants were isolated on the regeneration plates. Of the 363 fusants isolated, over 100 showed enhanced monacolin K production compared with the parental strain M. anka M-3. Ten of them produced monacolin K about 1.6-fold of that M.anka M-3 does and the monacolin K titer of two fusants (F49 and F104) increased by about 1-fold. The monacolin K yields of F49 and F104 were 460 μg/mL and 457 μg/mL respectively. In optimized fermentation medium, the monacolin K titer of F49 reached 1216 μg/mL.     

用真空温压技术制备纳米金属铜块体材料

采用自悬浮-真空温压法制备纳米金属Cu块体材料。通过X射线衍射(XRD)、扫描电镜(SEM)、透射电镜(TEM)、正电子湮没(PAS)实验、HMV-2型显微硬度计和用Arechimedes原理等测试手段研究压制压力、保压时间、温度等工艺参数对样品密度的影响;考察其热稳定性和微结构。研究结果表明:纳米金属Cu块体材料的密度随压制压力、保压时间和温度的增加而提高,最高相对密度为96.15%;纳米金属Cu块体材料具有较好的热稳定性;在1.5 GPa和200℃压制的样品的平均晶粒度为25.3 nm,微应变为0.047%。     

基于中值滤波去噪和小波融合的乳腺图像增强方法

将小波变换的多分辨率分析理论应用于图像融合中,提出了一种既能改善图像整体对比度,同时也能增强乳腺图像的组织细节结构的方法.实验结果说明了该方法的有效性.