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61.
Salvi M Toninello A Schweizer M Friess SD Richter C 《Cellular and molecular life sciences : CMLS》2002,59(7):1190-1197
Mitochondria contain a specific Ca2+ release pathway which operates when oxidized mitochondrial pyridine nucleotides are hydrolyzed. NAD+ hydrolysis and therefore Ca2+ release is possible when some vicinal thiols are cross-linked. Here we report that the thiol oxidant peroxovanadate inhibits
the specific Ca2+ release pathway. In mitochondria, peroxovanadate causes a complete loss of reduced glutathione, which is not accompanied
by formation of glutathione disulfide, and a partial loss of protein thiols. In model reactions, peroxovanadate oxidizes reduced
glutathione predominantly to the sulfonate derivative, but does not react with glutathione disulfide. When the vicinal thiols
relevant for Ca2+ release are cross-linked, Ca2+ release is no longer inhibited by peroxovanadate. Conversely, pretreatment of mitochondria with peroxovanadate makes them
insensitive to compounds promoting the disulfide state. These results suggest that peroxovanadate inhibits the prooxidant-induced
Ca2+ release from mitochondria by (i) depleting mitochondria of reduced glutathione and (ii) oxidizing the vicinal thiols relevant
for Ca2+ release to a state higher than disulfide, presumably the sulfonate state. The findings provide further insight into the regulation
of Ca2+ release from intact mitochondria, and may be relevant for a better understanding of the action of peroxovanadate in cells,
where the compound can be insulin mimetic.
Received 28 March 2002; received after revision 8 May 2002; accepted 15 May 2002 相似文献
62.
田敏 《西北大学学报(自然科学版)》1997,27(1):35-39
痕量锗、钛和钼与邻苯二酚紫(PV)和溴化十六烷基三甲胺(CTMAB)在酸性介质中发生高灵敏的显色反应,所产生的三元络合物的吸收光谱严重重叠。用最小二乘法—分光光度法成功地测定了模拟样的上述3种痕量组分。结果表明,最小二乘法是化学计量学中一种可适用于较为复杂的痕量组分同时分光光度测定的优良的计算方法。 相似文献
63.
Conclusion In this study, P23 was found to be a disulfide-bonded cytoplasmic protein, abundant in late exponential phase and stationary
phase cells, and was hardly detected in early exponential phase cells, the cells of sporulation process and spores. Therefore,
synthesis of P23 was regulated by some specific factors or/and cellular environment. Conclusively, cytoplasm has a mechanism
to catalyze the formation of disulfide bonds, which is consistent with Dermanet al. ’s conclusion from mutation experiment[1]. 相似文献
64.
润滑油多功能添加剂的研制 总被引:2,自引:0,他引:2
本文简述了润滑油多功能添加剂二烷基二硫代磷酸盐(ZDDP+MoDTP)合成工艺,作用机理,并对其主要技术指标进行测试。润滑性能模拟评定结果表明该剂极压抗展性能良好,是一种高档润滑油的添加剂 相似文献
65.
66.
2,2’-二硫联二苯并噻唑的电化学合成 总被引:3,自引:0,他引:3
以KI-NaI为间接电解质,丙酮为溶剂,首次用间接电氧化的方法对2-巯基苯并噻唑进行偶联,高产率地得到了2,2’-二硫联二苯并噻唑. 相似文献
67.
以大肠杆菌XL1blue为模板,通过PCR技术扩增大肠杆菌硫氧还蛋白基因,并将目的基因分别连接到克隆载体pUC18和表达载体pTrcHisC上,构建重组质粒.重组质粒pTrcHisC-TRX在大肠杆菌中高效表达,最后利用固定化金属螫合亲和层析技术(IMAC)获得较纯的目的蛋白,为进一步研究硫氧还蛋白的功能及其应用提供了条件. 相似文献
68.
本文介绍了Zn(Ⅱ)与巯基苯并噻唑的次磺酰胺衍生物DM、CZ的配合物的合成,并进行了IR、UV、元素分析等表征研究,指认所合成的化合物为[Zn(DM)CL_2](简称ZDM)、[Zn(CZ)_2CL_2](简称ZCZ)。 相似文献
69.
Dick TP 《Cellular and molecular life sciences : CMLS》2004,61(5):547-556
Assembly of functional major histocompatibility complex (MHC) class I peptide complexes within the endoplasmic reticulum is critically important for the development of an adaptive immune response. The highly regulated loading of peptides onto MHC class I molecules is controlled by a multi-component chaperone system called the MHC class I peptide loading complex. The recent identification of the thioredoxin family member ERp57 as a component of the loading complex led to an interesting question: Why is there a thiol-disulfide oxidoreductase inside a complex dedicated to inserting peptides into a receptor binding site? Most recently, specific ERp57-mediated disulfide bond rearrangements have been identified inside the loading complex. What these biochemical events mean for the peptide loading process remains a matter of conjecture. While several important questions wait to be answered, this review intends to summarize our current view of the oxidative folding of MHC class I molecules and addresses the question of how the receptor ligand interaction might be regulated by thiol-based redox reactions. 相似文献
70.
Cloning and activity analysis of<Emphasis Type="Italic">in vitro</Emphasis> expression of plant NAD-IDH genes 总被引:3,自引:0,他引:3
CHENDefu CHENXiwen 《科学通报(英文版)》2004,49(4):328-336