濒危植物桫椤过氧化物酶的分离纯化与性质

将齿叶黑桫椤叶片提取液经硫酸铵盐析、透析、DEAE-琼脂糖凝胶柱层析和Sephacryl S-200凝胶柱层析分离得到纯化的POD,其纯化倍数为26.29,回收率为27.04%;通过SDS-PAGE法测得POD的分子量为43000D;通过IEF-PAGE法测得POD的等电点为4.55;齿叶黑桫椤POD最适pH在4左右;最适温度在45℃左右;Km值在最适温度45℃、最适pH4的条件下为0.07 mol/L。     

甲醛对芦荟POD酶活性的影响

实验以玻璃箱模拟室内空间环境,应用乙酰丙酮分光光度法和邻苯三酚自氧化法测定在不同甲醛含量下芦荟对甲醛的吸收及其体内过氧化物酶(POD)活性的变化情况.结果表明,有芦荟存在时甲醛气体含量显著下降,芦荟体内的POD酶活性增强;随着通入甲醛气体量的增加,芦荟对甲醛的吸收量显著增加,POD活性的提高也越来越显著.说明芦荟体内POD酶对甲醛气体的胁迫存在着一定程度的生理应激反应.     

胃癌高发区不同人群血浆总抗氧化力及谷脱甘肽过氧化物酶活性的研究

用微弱化学发光法和酶联分光光度技术研究胃癌高发区－－甘肃省武威地区不同人血浆总抗氧化力（TRAC）和谷胱甘肽过氧化物酶（GSH－Px）活性，结果表明：中度表浅性胃炎18例、21例明显降低(p-≤0．01），但各组间血浆GSH－Px活性无显著性差异（P＞0．05），提示当地胃癌高发可能与血产供销 TRAC水平的降低有关，而与GSH－Px活性改变无明显相关。     

苹果果实大小与膜透性、过氧化物酶活性及丙二醛含量关系的研究

本文分析了苹果果实后熟衰老期间膜透性、过氧化物酶活性及丙二醛含量。结果表明,苹果果实大小不影响其过氧化物酶活性高低,但大果具有较大的过氧化物酶活性变化幅度,中等果具有较低小的过氧化物酶变化幅度。大果比中等果具有较高的膜透性与丙二醛含量。     

大球盖菇过氧化物酶及超氧化物歧化酶的研究

采用聚丙烯酰胺凝胶圆盘电泳法测定大球盖菇过氧化物酶和超氧化物歧化酶（SOD）同工酶活性,结果表明大球盖菇过氧化物酶有4种同工酶,比移分别是：0.13、0.18、0.25、0.32,其活性大小接近.SOD三种都有,比移分别为0.20、0.21、0.25,以Cu·Zn-SOD活性最大,Mn-SOD活性较小.Cu·Zn-SOD及Mn-SOD辅因子易于丢失,应用时应予以注意.     

真菌诱导物对丹参毛状根氧化酶活性的影响

从一种广谱病原真菌－大丽轮枝菌的菌丝体中制备激发子，用该激发子和商品生物制剂－酵母提取物分别诱导对数生长期的丹参毛状根，发现丹参毛状根过氧化物酶（POD），过氧化氢酶（CAT）和多酚氧化酶（PPO）活性在诱导后均显著增加，三种酶的活性并随诱导时间和诱导剂的浓度的增加而改变，大丽轮枝菌激发子的作用强于酵母提取物，表现为诱导酶活性峰值较高和峰值时间提前。     

Changes of Soil Enzyme Activities in Different Restoration Ages of Spruce Forests on the Eastern Qinghai-Tibet Plateau

Six soil enzymes (invertase, acid phosphatase, proteinase, catalase, peroxidase and polyphenoloxidase ) were chosen for investigation under different spruce forests with restoration ages of 4,10,16 years and an old-growth spruce forest over 400 years old in the eastern Qinghai-Tibet Plateau, China. Results showed that the activities of invertase, phosphatase, proteinase, catalase and peroxidase decreased in newly restored forests except for pholyphenoloxidase. With the development of forests after restoration, the activities of invertase, acid phosphadase, proteinase increased gradually. Our study also indicated that the soil enzyme activities were associated with surface soils and decreased with depths. This result suggested that in the earlier restoration stage the application of organic fertilizer may be more effective by surface addition to soils than deep addition.     

植物过氧化物酶的结构、催化反应及生理功能

过氧化物酶(EC.1.11.1.7)是一种以H_2O_2作为电子受体催化底物的氧化作用的酶,这种酶广泛存在于生物体中,这种酶已被广泛研究。本文讨论了过氧化物酶的化学结构、过氧化物酶的亚基构成、它们的催化反应及机理,同时对其的生理功能也作了较祥细的讨论。     

Biosynthesis of selenosubtilisin: A novel way to target selenium into the active site of subtilisin

Glutathione peroxidase （GPx, EC1.11.1.9）, an important anti-oxidative selenoenzyme, can catalyze the reduction of harmful hydroperoxides with concomitant glutathione, thereby protecting cells and other biological issues against oxidative damage. It captures considerable interest in redesign of its function for either the mechanism study or the pharmacological development as an antioxidant. In order to develop a general strategy for specifically targeting and operating selenium in active sites of enzymes, the catalytically essential residue selenocysteine （Sec） was first successfully bioincorporated into the catalytic center of subtilisin by using an auxotrophic expression system. The studies of the catalytic activity and the steady-state kinetics demonstrated that selenosubtilisin is an excellent GPx-like biocatalyst. In comparison with the chemically modified method, biosynthesis exhibits obvious advantages： Sec could be site-directly incorporated into active sites of enzymes to overcome the non-specificity generated by chemical modification. This study provides an important strategy for specifically targeting and operating selenium in the active site of an enzyme.     

抗坏血酸对大豆某些生理指标的影响

在相等土壤肥力和相同体积花盆中培养的大豆幼苗,分别用浓度5、10、20mmol.L-1的抗坏血酸(VC)和自来水(CK)浇灌,18d后逐步测定正在扩张生长期的叶片中可溶性糖、可溶性蛋白含量、硝酸还原酶(NRA)、过氧化物酶(POD)、超氧化物歧化酶(SOD)的活性.结果表明:10mmol.L-1 VC处理的大豆叶片可溶性糖、可溶性蛋白含量,SOD酶活性比CK均有所提高;3个处理的硝酸还原酶(NRA)活性均比CK有所增大;10mmol.L-1 VC处理对POD酶活性影响不明显.