Melatonin regulation of antioxidant enzyme gene expression

Antioxidant enzymes (AOEs) are part of the primary cellular defense against free radicals induced by toxins and/or spontaneously formed in cells. Melatonin (MLT) has received much attention in recent years due to its direct free radical scavenging and antioxidant properties. In the present work we report that MLT, at physiological serum concentrations (≈ 1 nM), increases the mRNA of both superoxide dismutases (SODs) and glutathione peroxidase (GPx) in two neuronal cell lines. The MLT effect on both SODs and GPx mRNA was mediated by a de novo synthesized protein. MLT alters mRNA stability for Cu-Zn SOD and GPx. Experiments with a short time treatment (pulse action) of MLT suggest that the regulation of AOE gene expression is likely to be receptor mediated, because 1-h treatment with MLT results in the same response as a 24-h treatment. Received 18 June 2002; received after revision 5 August 2002; accepted 27 August 2002 RID="*" ID="*"Corresponding author.     

The TSH receptor and its role in thyroid disease

The thyrotropin (TSH) receptor plays a preeminent role in thyroid physiology and disease. TSH, acting through the TSH receptor, is the major stimulator of thyroid cell growth, differentiation and function. In Graves' disease, the TSH receptor is the target of stimulating antibodies that cause hyperthyroidism. Although still a topic of debate, the TSH receptor has been implicated in the pathogenesis of the endocrine ophthalmopathy associated with Graves' disease. Blocking antibodies against the TSH receptor are involved in the development of hypothyroidism in a subset of patients with autoimmune hypothyroidism. Transplacental passage of stimulating or blocking TSH receptor antibodies from a mother with autoimmune thyroid disease may result in transient hyper- or hypothyroidism in early infancy. During pregnancy, the placental hormone human choriogonadotropin (hCG) can cause gestational hyperthyroidism through cross-reaction with the TSH receptor. Gestational hyperthyroidism may also be involved in the pathogenesis of hyperemesis gravidarum. Trophoblast tumors secreting hCG are a rare cause of hyperthyroidism. Somatic activating mutations of the TSH receptor have been identified as a molecular cause of toxic adenomas, whereas activating mutations in the germline give rise to nonautoimmune familial hyperthyroidism or sporadic congenital hyperthyroidism. These gain-of-function mutations are dominant, and one mutated allele is sufficient to result in disease. Inactivating germline mutations of both TSH receptor alleles lead to variable degrees of resistance to TSH, encompassing a spectrum ranging from euthyroid hyperthyrotropinemia to overt hypothyroidism with thyroid hypoplasia. Received 31 January 2001; received after revision 3 April 2001; accepted 3 April 2001     

心率变异性的非线性分析——人体心搏的混沌特性

用自制的心电信号测试系统，采集了健康人和心脏病患者的心搏数据，用非线性方法对心率变异性（ＨＲＶ）进行了较全面的分析。研究结果表明：混沌特性是年轻健康心脏的重要标志，而多变性的降低或周期性的增加往往伴随着衰老和疾病。     

35CrMo耐热钢的激光焊接

采用８００ＷＣＯ２激光器对３５ＣｒＭｏ耐热钢进行了深熔焊接研究，得到了最佳的焊接工艺参数，讨论并分析了各工艺参数及其它因素对激光焊接质量、性能的影响。     

自血光量子疗法治疗肺性脑病30例疗效分析

光量子疗法（AVIB）是近年来发展起来的一种新的治疗手段，本文对我院应用自身光量子疗法治疗肺性病30例，临床观察分析经与对照组30例比较，证明其疗效满意，临床可行性可靠性均较好，为一种有效的极物异性新疗法，有推广应用价值。     

中医对亚健康的研究概况

介绍中医对亚健康的研究概况，包括中医对亚健康的认识，以及亚健康的分类治疗，并对研究现状进行了评价，旨在促进中医对亚健康的研究。     

大学生神经衰弱的病因分析

对大学生１６４例神经衰弱的发病因素分析表明，家族遗传缺陷以及社会心理因素与该病的发生前关。通过心理疏导、神经支持等措施，并采用中西医结合治疗后症状逐渐消失。     

人体耳穴频率响应特性测试及其与疾病相关性分析

提出一种无创伤疾病诊断方法，通过人体耳穴测试人体系统频率响应特性，运用计算机进行统计分析处理，研究耳穴频响特性与疾病的相关性，获取一种反映人体各部生理信息的特征参数。     

楮时资源利用价值初探──Ⅱ．提取物的抗氧化作用研究

为寻找质优价廉、丰富易得，并能防病治病的天然食品抗氧剂，本文中采用“烘箱法”对楮叶的醇提取物和水提取物在花生油中的抗氧化作用进行了考察。结果表明，两种提取物的添加量为０．６％时，其抗氧化作用均明显高于Ｖｃ（５００μｇ／ｇ）的抗氧化作用，且醇提取物的抗氧活性高于水提取物的抗氧活性。     

中国人P型铜转运ATP酶基因突变的初步研究

应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）及测序等方法分析了１４１便ＷＤ患者ＡＴＰ７Ｂ基因第７、９及１４外显子的部分ＤＮＡ序列改变，通过对患者ＤＮＡＴ下沉人ＤＮＡＲＰＣＲ－ＳＳＣＰ电泳带迁移作对比分析，发现在１４１例患者中第７、９及１４外显子ＰＣＲ扩增产物迁移异常才分别为４例、６例和４０例，依据ＤＮＡ物单链构聚与分子电泳迁移的关系，提示该组病人中ＡＴＰ７Ｂ基因第７、９和１４外显子的突变率