On the mechanism of inhibition of p27 degradation by 15-deoxy-Δ12,14-prostaglandin J 2 in lymphoblasts of Alzheimer’s disease patients

It has been proposed that neuroinflammation, among other factors, may trigger an aberrant neuronal cell cycle re-entry leading to neuronal death. Cell cycle disturbances are also detectable in peripheral cells from Alzheimer’s disease (AD) patients. We previously reported that the anti-inflammatory 15- deoxy-Δ^12,14-prostaglandin J ₂ (15d-PGJ ₂) increased the cellular content of the cyclin-dependent kinase inhibitor p27, in lymphoblasts from AD patients. This work aimed at elucidating the mechanisms of 15d-PGJ ₂-induced p27 accumulation. Phosphorylation, half-life, and the nucleo-cytoplasmic traffic of p27 protein were altered by 15d-PGJ2 by mechanisms dependent on PI3K/Akt activity. 15d-PGJ ₂ prevents the calmodulin-dependent Akt overactivation in AD lymphoblasts by blocking its binding to the 85-kDa regulatory subunit of PI3K. These effects of 15d-PGJ ₂ were not mimicked by 9,10-dihydro-15-deoxy-Δ^12,14- prostaglandin J ₂, suggesting that 15d-PGJ ₂ acts independently of peroxisome proliferator-activated receptor γ activation and that the α,β-unsaturated carbonyl group in the cyclopentenone ring of 15d-PGJ ₂ is a requisite for the observed effects. Received 14 July 2008; received after revision 2 September 2008; accepted 12 September 2008     

摩擦填料作用下土工充砂袋充填特性

为更准确计算土工充砂袋在充填完成时袋体形状及受力特性,针对袋体与充填料之间的摩擦对袋体的影响,通过在计算中引入填料与袋体间的摩擦,建立袋体的平衡方程,运用数值方法对方程进行求解.将袋体形状及张力与不考虑袋体与充填料之间的摩擦时的计算结果进行比较,发现袋体的形状及受力特性有显著的不同,同样条件下摩擦系数越大,充填完成时袋体的高度越大,最大宽度及触地宽度越小;袋体张力也不再是常数,而是随土工袋切角的增加而增大,最大张力远大于不考虑摩擦时的张力.     

层流液柱吸收法测定气体在液相中的扩散系数

采用射流吸收的简捷方法，通过测定由Ｓｈｏｕｔｈｗｅｌｌ型孔板所产生的截面速度均匀的层流喷射液柱所吸收的气体量，由渗透理论所导出的模型，可计算出一定温度、压力下的微溶气体在液相中的分子扩散系数Ｄ。在温度为３０～４５℃的范围，测试结果与文献值吻合良好．     

保E增序完全变换的变量半群上的格林*关系

给出了保E增序完全变换的变量半群上的格林*关系的刻画及其性质.     

教育在贫困地区促农增收中存在的问题与对策——基于云南省广南县大革假村的调查与分析

教育促进农民增加收入的作用不够明显,与贫困地区的社会经济发展状况及教育自身存在的问题不无关联。根据分析,普遍较低的受教育程度、教育费用负担、高学历人员的流失、课程的适应性不够等方面都可能是造成这一现状的原因,并针对这些问题提出了相应的对策。     

回民小调、河湟“花儿”与陕北“信天游”的音乐渗透

回民小调、河湟“花儿”与陕北“信天游”在河北、山西以及西北广大地区互相渗透交汇,形成了一种彼此交错分布、你中有我、我中有你的音乐文化交融格局。     

4-Hydroxynonenal-modified amyloid-beta peptide inhibits the proteasome: possible importance in Alzheimer's disease

The amyloid β-peptide (Aβ) is a 4-kDa species derived from the amyloid precursor protein, which accumulates in the brains of patients with Alzheimer’s disease. Although we lack full understanding of the etiology and pathogenesis of selective neuron death, considerable data do imply roles for both the toxic Aβ and increased oxidative stress. Another significant observation is the accumulation of abnormal, ubiquitin-conjugated proteins in affected neurons, suggesting dysfunction of the proteasome proteolytic system in these cells. Recent reports have indicated that Aβ can bind and inhibit the proteasome, the major cytoslic protease for degrading damaged and ubiquitin-conjugated proteins. Earlier results from our laboratory showed that moderately oxidized proteins are preferentially recognized and degraded by the proteasome; however, severely oxidized proteins cannot be easily degraded and, instead, inhibit the proteasome. We hypothesized that oxidatively modified Aβ might have a stronger (or weaker) inhibitory effect on the proteasome than does native Aβ. We therefore also investigated the proteasome inhibitory action of Aβ _1–40 (a peptide comprising the first 40 residues of Aβ) modified by the intracellular oxidant hydrogen peroxide, and by the lipid peroxidation product 4-hydroxynonenal (HNE). H₂O₂ modification of Aβ _1–40 generates a progressively poorer inhibitor of the purified human 20S proteasome. In contrast, HNE modification of Aβ _1–40 generates a progressively more selective and efficient inhibitor of the degradation of fluorogenic peptides and oxidized protein substrates by human 20S proteasome. This interaction may contribute to certain pathological manifestations of Alzheimer’s disease Received 26 September 2000; accepted 26 September 2000     

阴离子聚合物制备及其在湿敏元件方面的应用

通过自由基溶液聚合制得丙烯酸（ＡＡ）－丙烯酸丁酯（ＢＡ共聚物（Ｐ（Ａ—ＢＡ）），利用ＬＩＯＨ溶液中和制得中和度为的％的阴离子聚合物（Ｐ（Ａ—ＢＡ—Ｌｉ））。用此离聚物为主要湿敏材料制作出具有良好耐水性的湿敏元件。当相对湿度（ＲＨ）大于４０％时，该元件阻抗的对数与相对湿度成线性关系；ＲＨ由４３％提高到９７％时，元件阻抗的变化值达３个数量级，吸湿响应时间≤１０秒。     

工程中瞬态温度场的有限元分析

本文考虑瞬态温度场问题，先后将空间域和时间域离散，从具有更广泛意义的加权剩余法导出瞬态温度场的有限元方程，应用等参数单元分析瞬态温度场．     

二阶退化双曲型方程的第二类广义Darboux问题

A.V.Bitsadze在文[1]中提出和研究了二阶一致线性双曲型方程uxx-uyy+aux+by+cu+d=0(A)的第一类和第二类Darboux问题.本文的目的是讨论二阶退化双曲型方程第二类广义Darboux问题和斜微商问题解的表示式,并证明这些问题解的存在唯一性。本文使用不同于[1]中的方法,但类似于[1]中的方程(A),根据本文中的结果,我们可以解决广义Chaplygin方程在一般区域上的Frankl问题.