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41.
A. M. Fenyves M. Saxer K. Spanel-Borowski 《Cellular and molecular life sciences : CMLS》1994,50(2):99-104
Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently. 相似文献
42.
Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10–10 M–10–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors. 相似文献
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45.
介绍了以鸡冠花为原料,提取其红色素的方法及工艺流程。通过光谱分析的手段,研究了酸度、温度、光、时间、金属离子等因素对色素稳定性的影响。 相似文献
46.
牛蒡中多酚氧化酶的活性及其影响因素研究 总被引:6,自引:0,他引:6
对牛蒡中所含的多酚氧化酶的活性及其影响因素的研究表明,牛蒡中多酚氧化酶的适宜pH值是6.0~7.5,适宜温度45℃,较有效的抑制剂是2%钾明矾和0.02%SSA的混合液,或0.58%磷酸和0.02%SSA的混合液.用此溶液浸泡过的牛蒡,再用HPE薄膜袋包装后,在-40℃下速冻,放在-18℃冷冻库保存,贮藏期达6个月以上. 相似文献
47.
小菜蛾Plutella xylostella羧酸酯酶、酰胺酶及乙酰胆碱酯酶的研究 总被引:2,自引:0,他引:2
研究结果表明,小菜蛾(Plutella xylostella)乙酰胆碱酶、酰胺酶的最适pH值分别为8.0和8.5。pH值对闳酸酯酶活力影响较小,酰胺酶和羧酸酯酶反应速度在反应开始60钟内,仍在线性范围,乙酰胆碱酯酶反应速度在反应开始的8分钟内呈线性关系。羧酸酯酶、酰胺酶和乙酰胆碱酯酶的Vmax分别为551.81(nmol/mg,mln)、5.25×10~(-4)(μmol/mg.min)和20.36(nmol/mg.min);km分别为5.52×10~(-5)(mol)、1.18×10~(-3)(mol)和8.33×10~(-3)(mol)。 相似文献
48.
盐胁迫下灰绿藜叶片光合特性与叶绿体离子调节的研究 总被引:5,自引:0,他引:5
冯立田 《曲阜师范大学学报》1998,24(3):57-61
NaCl处理后,灰绿藜整个叶片内N^+a和Cl&-积累,但叶绿体内仅少量增加。叶绿体与叶片的K^+浓度都降低。 相似文献
49.
The effects of calmoddin (CaM) antagonist W7-agarose, anti-CaM serum and exogenous purified CaM on pollen germination and
tube growth ofForsythia suspensu were studied. The pollen germination and tube growth were inhibited or completely stopped by CaM antagonist W7-agarose. The
addition of exogenous purified CaM stimulated pollen germination and tube growth, whereas the same amount of bovine serum
albumin (BSA) had no effect. The inhibitory effects caused by W7-agarose and anti-CaM serum could be reversed completely by
the addition of exogenous purified CaM. The tube growth of germinated pollen was also inhibited or completely stopped by W7-agarose.
The results suggest that endogenous extracellular CaM initiates pollen germination and tube growth, whereas exogenous CaM
enhances the above processes. 相似文献
50.
用X射线原位应力测定了高温氧化膜Cr2O3和NiO中的应力.结果表明,Cr2O3/Cr和NiO/Ni体系氧化膜的生长应力分别为-1.949GPa和652MPa.在冷却过程中,Cr2O3/Cr体系发生很大的应力松弛过程,其主要表现形式为氧化膜翘曲和开裂;而在NiO/Ni体系中,由于NiO薄膜具有双层结构承受不同应力状态而获得松弛. 相似文献