High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp. indica and japonica) to abiotic stress conditions

Rice (Oryza sativa L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm PLUS and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.     

旅游消费在怎样的收入区间才有可能

旅游消费作为一种享受型生活，并不是在什么收入下都可以从消费心理变成消费行为的。它必须要收入达到了在“消费－－储蓄”曲线上消费曲线与储蓄曲线的交点以后，即收入水平达到小康以后。     

百分数抽检的不合理性

本文利用抽检特征曲线，分析百分数抽检的不合理性，并给出了改进的方法。     

气流阻力负荷对阻塞性睡眠呼吸暂停患者流速容量曲线的影响

探讨加用气流阻力负荷（ＦＲＬ）状态下流速－容量（Ｆ－Ｖ）曲线检查对初检ＯＳＡ患者的意义。对象为２４名健康成年男性及２１例男性阻塞性睡眠呼吸暂停（ＯＳＡ）患者。经整夜多导睡眠仪检查诊断或排除ＯＳＡ。模拟实验结果发现只有同时加上可陷闭管及ＦＲＬ时，才出现显著吸气气流受限。无ＦＲＬ时，Ｆ－Ｖ曲线（以ＦＥＦ５０／ＦＩＦ５０比值大于１为指标）检查发现ＯＳＡ的敏感性和特异性分别为３３．３％和９１．６％，阳性预     

企业会计新准则的构架分析及实施

新准则与现行准则相比较，无论是适用范围、无形资产的定义、还是无形资产的确认、计量、记录以及披露均有不同程度的修订和变化。从整体构架来看，新准则内容较现行准则结构更加严谨、内容更加科学和规范，它预示着我国企业会计准则进一步走向成熟化和国际化。     

H~2(T~2)上的Toeplitz算子

给出了H2 (T2 )上Toeplitz算子的特征方程 :T zTTz =T ,T wTTw =T ,及两个Toeplitz算子 φ ,ψ∈L∞(T2 ) ,Tφ 和Tψ 的乘积TφTψ 仍为Toeplitz算子的充要条件是 :φ对z、w中零个、一个或两个变量共轭解析 ,ψ对余下变量解析 ,且乘积为Tφψ。     

高等级公路隧道照明工程设计与研究

结合公路隧道的视觉特点,探讨了照明设计的原则和标准,并通过对照明曲线的分析,提出了改进方法,给出了改进后的照明曲线.     

空间有理三次Bēzier曲线参数化方法

研究在曲线形状保持不变的条件下,空间有理三次Bēzier曲线权因子改变与曲线重新参数化的关系.给出了空间有理三次Bēzier曲线上点的参数与权因子之间的对应关系,并导出权因子改变对空间有理三次Bēzier曲线参数化有影响的参数变换公式.     

Campanato空间上的Hardy—Littlewood极大算子

证明了Ｈａｒｄｙ－Ｌｉｔｔｌｅｗｏｏｄ极大算子Ｃａｍｐａｎａｔｏ空间上的有界性。     

自然循环两相流动不稳定性的判别准则

针对两相自然循环流动不稳定性的特点，建立了一个集总参数模型．通过无量纲化、小扰动及理论分析，得到了两相自然循环流动不稳定性的判别准则．结果与文献符合较好．