Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis

A new feather-degrading bacterium was isolated from a local feather waste site and identified as Bacillus subtilis based on morphological, physiochemical, and phylogenetic characteristics. Screening for mutants with elevated keratinolytic activity using N-methyl-N＇-nitro-N-nitrosoguanidine mutagenesis resulted in a mutant strain KD-N2 producing keratinolytic activity about 2.5 times that of the wild-type strain. The mutant strain produced inducible keratinase in different substrates of feathers, hair, wool and silk under submerged cultivation. Scanning electron microscopy studies showed the degradation of feathers, hair and silk by the keratinase. The optimal conditions for keratinase production include initial pH of 7.5, inoculum size of 2% （v/v）, age of inoculum of 16 h, and cultivation at 23 ℃. The maximum keratinolytic activity of KD-N2 was achieved after 30 h. Essential amino acids like threonine, valine, methionine as well as ammonia were produced when feathers were used as substrates. Strain KD-N2, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production.     

C8051F020单片机应用于船用减摇鳍控制系统的SFR初始化

归纳了C8051F020的122个特殊功能寄存器(SFR)的复位状态，对需做初始化的SFR进行了排序，给出了应用于船用减摇跨控制系统的初始化程序(中文注释)范例，并结合范例程序注释了对关键SFR配置内涵的理解，讨论了集成开发环境(IDE)中配置向导的使用方法。     

内置BS接收画中画的三高频调谐彩色电视机研制

内置ＢＳ接收画中画的三高频调谐彩色电视机研制李元密，陈皓光，张毅（厦门大学电子工程系）（厦门达真磁记录有限公司）卫星电视广播是解决幅员辽阔和地形复杂所造成的电视复盖率低、节目源少的最好途径。随着“东方红号”和“亚洲一号”广播卫星的发射，必将导致卫星电...     

基于DSP56F807对EEG的FIR陷波实现

本文在基于高速DSP处理芯片Motorola DSP56FS07的佩带式EEG回馈仪上实现采用Kaiser窗的500阶FIR数字陷波器，并进行了计算机仿真与硬件实现。     

Functional and physical communication between oncoproteins and tumor suppressors

The discovery of oncogenes (c-onc’s) and tumor suppressors (TS’s) has led to the concept that cancer arises from defects in each of these classes of genes or their products. More recently, it has been appreciated that c-onc and TS proteins often affect one another’s functions. Within this context, I review the two classical TS’s, p53 and the retinoblastoma protein, and the consequences of their inactivation. The various forms of genomic instability (GI) that underly the high mutation rates of transformed cells are then discussed. Particular emphasis is placed upon the concept that GI is not only an integral part of the transformed state but is a prerequisite. Increased oxidative DNA damage, and/or an inabiliy to repair it, can lead to GI. The review then discusses recent observations showing that loss of the TS protein peroxiredoxin 1 (prdx1) and increased expression of the c-onc protein c-Myc, each leads to increased oxidative DNA damage. The critical nature of the c-onc-TS interaction is underscored by that occurring between prdx1 and c-Myc, with the former protein regulating the production of DNA-damaging reactive oxygen species by the latter. The intimate association between these proteins and others serves as a paradigm for the exquisite balancing act that c-onc’s and TS’s must maintain in order to properly control normal DNA replication and cellular proliferation while simultaneously minimizing the acquisition of potentially neoplastic mutations. Received 10 May 2005; received after revision 3 July 2005; accepted 19 July 2005     

HAMILTONICITY IN 2-CONNECTED {K_(1,3) F}-FREE GRAPHS

ＨＡＭＩＬＴＯＮＩＣＩＴＹＩＮ２－ＣＯＮＮＥＣＴＥＤ｛Ｋ_（１，３）Ｆ｝－ＦＲＥＥＧＲＡＰＨＳ￥ＬＩＧｕｏｊｕｎ（ＭａｔｈｅｍａｔｉｃｓＤｅｐｅｒｔｍｅｎｔｏｆＹａｎｔａｉＴｅａｃｈｅｒ＇ｓＣｏｌｌｅｇｅ，Ｙａｎｔａｉ２６４０００，Ｃｈｉｎａ）ＬＩＵ?..     

快速消解加热法测定水中高锰酸盐指数

用HCA-10O标准消解器进行快速加热,控制加热时间,并设有风冷加水冷的冷凝回流,同时减小加药体积,快速消解加热测定了地表水的高锰酸盐指数,该方法缩短了实验时间,短时快速测定出高锰酸盐指数,确定了稀释倍数,避免了多次反复稀释,提高了工作效率,适应了日益繁重的水质监测工作要求.     

冶金级硅酸洗提纯研究

冶金工艺被认为是非常有前途的一种有别于传统的西门子工艺而且能够满足市场对太阳能级硅需求的工艺.冶金级硅的预处理是冶金提纯工艺路径中非常重要的一个环节,因为酸洗可以有效地去除硅中的金属杂质例如铝、铁和钙等.通过系统性的对比试验对酸洗工艺去除硅中杂质的效果进行了详细的研究,分别探讨了酸的种类、酸的浓度、酸洗时间、酸洗温度以及硅粉颗粒粒径对杂质去除效率的影响.研究结果表明最佳的酸洗工艺参数为15%的盐酸,80℃,10h和100μm.在最佳酸洗工艺参数条件下金属杂质铝、铁和钙的去除率分别为70.90%、94.82%和82.69%.     

Sr3AlO4F晶体中掺杂Ba2+,Ca2+离子占据不同Sr2+格位倾向性的第一性原理研究

通过比较第一性原理计算总能量得出碱金属离子Ba2+和Ca2+取代占据氟氧化物晶体Sr3AlO4F中两种不同Sr格位时,Ba2+倾向占据10配位的Sr(1)位,而Ca2+倾向占据8配位的Sr(2)位,与实验分析结果一致.基于上述优化结构,通过键价求和计算和畸变指数分析,得出Sr3AlO4F中Sr(1)格位离子严重未饱和成键,倾向于被较大Ba2+离子取代占据;而Sr(2)格位离子略微过饱和成键,倾向于被较小Ca2+离子取代占据.     

骨桥蛋白在免疫中的作用

骨桥蛋白（OPN）是一种多功能的磷酸化糖蛋白,其涉及多种病理生理学过程.近来研究发现骨桥蛋白通过不同的翻译起始位点产生两种形式的蛋白,即分泌型骨桥蛋白（ sOPN,secreted OPN）和胞内型骨桥蛋白（iOPN,intracellular OPN）.本文主要介绍了sOPN及iOPN在免疫应答中的作用.