全文获取类型
收费全文 | 4654篇 |
免费 | 130篇 |
国内免费 | 252篇 |
专业分类
系统科学 | 56篇 |
丛书文集 | 140篇 |
教育与普及 | 35篇 |
理论与方法论 | 10篇 |
现状及发展 | 155篇 |
研究方法 | 1篇 |
综合类 | 4636篇 |
自然研究 | 3篇 |
出版年
2024年 | 4篇 |
2023年 | 17篇 |
2022年 | 33篇 |
2021年 | 35篇 |
2020年 | 48篇 |
2019年 | 39篇 |
2018年 | 38篇 |
2017年 | 44篇 |
2016年 | 48篇 |
2015年 | 88篇 |
2014年 | 190篇 |
2013年 | 172篇 |
2012年 | 250篇 |
2011年 | 236篇 |
2010年 | 237篇 |
2009年 | 329篇 |
2008年 | 322篇 |
2007年 | 386篇 |
2006年 | 376篇 |
2005年 | 306篇 |
2004年 | 273篇 |
2003年 | 247篇 |
2002年 | 230篇 |
2001年 | 151篇 |
2000年 | 141篇 |
1999年 | 124篇 |
1998年 | 82篇 |
1997年 | 91篇 |
1996年 | 72篇 |
1995年 | 64篇 |
1994年 | 59篇 |
1993年 | 58篇 |
1992年 | 55篇 |
1991年 | 51篇 |
1990年 | 44篇 |
1989年 | 26篇 |
1988年 | 30篇 |
1987年 | 13篇 |
1986年 | 10篇 |
1985年 | 12篇 |
1984年 | 3篇 |
1955年 | 2篇 |
排序方式: 共有5036条查询结果,搜索用时 125 毫秒
251.
研究了含钴NdFeB系列稀土永磁体的磁性能与显微组织,Co加入到三元NdFeB磁体中显著提高磁体的居里温度Tc,因而大大改善了可逆温度系数α(Br).对实验数据的计算机拟合表明二者间符合指数函数关系,且在单对数坐标中表现为两阶段曲线.在多组元NdFeB合金系列中,Al是提高含Co磁体矫顽力最有效的元素,其次是Mo,而Nb和V则不能改善Co对矫顽力的有害影响.高Co磁体中,Al恶化α(Br)的作用已非主要,这是由于Co提高Tc的作用大于Al对Tc的降低作用,但含Al磁体的β(fHC)较大,导致fHc随温度上升而迅速下降.含Nb、Mo、V磁体的β(fHc)则显著优于含Al磁体. 相似文献
252.
自高T_c氧化物超导陶瓷出现以来,已提出了许多可能与超导电性相关的因素。为探讨这些因素对超导性的影响,对元素掺杂的研究有着重要的意义。按照容许因子公式的要求,我们选择了满足ABO_3化合物中B位要求条件下的过渡族元素,将它们掺入(YBa)CuO_3中时,能部分取代Cu的位置而处于B位上。这样利用B位元素的少量掺杂,可以作为“结构探针”引出与周围O的2P电子、氧缺位及Cu、稀土离子等相互作用信息的变化,有助于对超导微观机理的了解;又因为ABO_3化合物 相似文献
253.
以2-氰基-4-硝基苯胺为原料合成了3-氨基-5-硝基-2,1-苯并异噻唑,并使之与邻异丙基苯酚偶联,得到2-[3-(5-硝基苯并异噻唑)偶氮]-6-异丙基苯酚(5-NO2BPAIP),测得其pKa为7.08.光度法和pH滴定表明它有可能成为一个敏锐的酸碱指示剂和Cu2+、Co2+、Ni2+等离子的显色剂。 相似文献
254.
宣士斌 《广西民族大学学报》1995,(1)
本文主要考察确定性k值随机微分方程(Ⅰ)dxt=Axtdt和关于H值C.B.m的随机微分方程(Ⅱ)dxt=AXtdt+G(Xt)dBt及(Ⅲ)dxt=AXtdt+G(Xt-Xt-τ)dBt(其中τ为充分小常量)的几种稳定性之间的关系 相似文献
255.
在酸性溶液中,钒钼杂多酸根与罗丹明6G生成离子缔合物,使罗丹明6G荧光定量淬灭,建立了微量钒荧光分析新方法,该方法灵敏度高,选择性好,工作曲线线性范围为0~2.5μg/25cm3,操作简便,用于钢样中微量钒的测定.效果较好。 相似文献
256.
采用简便的建立化学反应动力学方程的方法,研究了B90催化剂上丁烯氧化脱氢制丁二烯反应的动力学,即先在一较低温度下,用微分法拟合得到化学反应动力学方程中的浓度项,然后改变反应温度,求算不同温度下的反应速率常数。采用这个简便方法,只需较少的实验,即可求得该反应体系中的丁烯转化速率方程式。 相似文献
257.
Peptide aptamers have emerged as powerful new tools for molecular medicine. They can specifically bind to and functionally
inactivate a given target molecule under intracellular conditions. Typically, peptide aptamers are generated by screening
a randomized peptide expression library, displayed from the Escherichia coli thioredoxin A (TrxA) protein. Here, we transferred peptide moieties from defined TrxA-based peptide aptamers to alternative
scaffold proteins, such as the green fluorescent protein and staphylococcal nuclease. Yeast and mammalian two-hybrid assays
as well as in vitro binding analyses show that the TrxA scaffold can be a major determinant for the binding of peptide aptamers.
In addition, we demonstrate that TrxA can correctly display peptide sequences that correspond to the binding domains of natural
interaction partners. Therefore, sequence analyses of TrxA-based peptide aptamers, isolated by two-hybrid screening from randomized
expression libraries, should also be useful to find cellular binding partners for a given target protein, by homology.
Received 1 August 2002; received after revision 17 September 2002; accepted 19 September 2002
RID="*"
ID="*"Corresponding author. 相似文献
258.
Morphine 6 glucuronide stimulates nitric oxide release in mussel neural tissues: evidence for a morphine 6 glucuronide opiate receptor subtype 总被引:1,自引:0,他引:1
Mantione K Zhu W Rialas C Casares F Cadet P Franklin AL Tonnesen J Stefano GB 《Cellular and molecular life sciences : CMLS》2002,59(3):570-574
We have previously demonstrated that Mytilus edulis pedal ganglia contain opiate alkaloids, i.e., morphine and morphine 6 glucuronide (M6G), as well as mu opiate receptor subtype
fragments exhibiting high sequence similarity to those found in mammals. Now we demonstrate that M6G stimulates pedal ganglia
constitutive nitric oxide (NO) synthase (cNOS)-derived NO release at identical concentrations and to similar peak levels as
morphine. However, the classic opiate antagonist, naloxone, only blocked the ability of morphine to stimulate cNOS-derived
NO release and not that of M6G. CTOP, a mu-specific antagonist, blocked the ability of M6G to induce cNOS-derived NO release
as well as that of morphine, suggesting that a novel mu opiate receptor was present and selective toward M6G. In examining
a receptor displacement analysis, both opiate alkaloids displaced [3H]-dihydromorphine binding to the mu opiate receptor subtype. However, morphine exhibited a twofold higher affinity, again
suggesting that a novel mu opiate receptor may be present.
Received 1 November 2001; received after revision 1 February 2002; accepted 1 February 2002 相似文献
259.
The Ror receptor tyrosine kinase family 总被引:6,自引:0,他引:6
Forrester WC 《Cellular and molecular life sciences : CMLS》2002,59(1):83-96
Receptor tyrosine kinases (RTKs) participate in numerous developmental decisions. Ror RTKs are a family of orphan receptors
that are related to muscle specific kinase (MuSK) and Trk neurotrophin receptors. MuSK assembles acetylcholine receptors at
the neuromuscular junction [1, 2], and Trk receptors function in the developing nervous system (reviewed in [3-5]). Rors have
been identified in nematodes, insects and mammals. Recent studies have begun to shed light on Ror function during development.
In most species, Rors are expressed in many tissue types during development. Analyses of mutants that are defective in the
single nematode Ror demonstrate a role in cell migration and in orienting cell polarity. Mice lacking one of the two Ror gene
products display defects in bone and heart formation. Similarly, two different human bone development disorders, dominant
brachydactyly B and recessive Robinow syndrome, result from mutations in one of the human Ror genes.
Received 17 April 2001; received after revision 2 July 2001; accepted 4 July 2001 相似文献
260.
Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus 总被引:5,自引:0,他引:5
ZHANGLingdi WANGZhaohui WANGXianbing LIDawei HANChenggui ZHAIYafeng YUJialin 《科学通报(英文版)》2005,50(4):305-310
Functional analysis for gene silencing suppressor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- infiltration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP)expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by homologous sequence was strongly suppressed by the immixture infiltration of either the P14 or the $6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results suggested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors. 相似文献