基于STM32和嵌入式Web服务的智能温度监测系统

基于网络的远程测控系统应用越来越广泛,通过网络访问实时数据已经成为普遍要求,而温度是其中最具代表性的数据之一.设计了能够通过Web方式访问的温度监测系统.系统采用STM32作为MCU,通过DS18B20进行单总线多点测温,用ENC28J60以太网控制器搭建Web服务供远程监测,具有一定的实用价值,并且具有通用性和可移植性.     

“云南十八怪”品牌策略探析

"云南十八怪"品牌是云南省特有的品牌,但目前大多数"云南十八怪"产品的名号却不如"云南十八怪"这个传说来得响亮。根据调查,分析了"云南十八怪"品牌经营的整体现状、存在问题,最后为"云南十八怪"品牌的发展提出了一些借鉴策略。     

陕西“民国十八年年馑”巨灾的人祸因素分析

八十年前的“陕西民国十八年年馑“,是一场震惊中外的奇荒巨灾。这场灾难的发生,除了不可抗拒的自然因素外,更多地是由当时的社会环境和人为因素所造成的。从社会因素和人为因素入手,分析年馑的成因,可以得出陕西民国十八年年馑是人祸大于天灾的结论。     

Linking morphology and molecules: integrative taxonomy of spathidiids (Protista: Ciliophora: Litostomatea) from Korea

We isolated seven spathidiids from various terrestrial habitats in Korea: Enchelys megaspinata sp. nov.; Spathidium ascendens Wenzel, 1955; S. papilliferum Kahl, 1930; S. polynucleatum (Foissner et al., 2002) comb. nov.; S. rectitoratum Kahl, 1930; S. securiforme Kahl, 1930 stat. nov.; and Apobryophyllum schmidingeri Foissner and Al-Rasheid, 2007. Their vegetative and resting cyst morphology were studied in vivo and after protargol impregnation. Their phylogenetic relationships were inferred from the analysis of 18S rRNA gene and ITS1-5.8S-ITS2 region sequences. Enchelys megaspinata is nested within the Semispathidium breviarmatum cluster. It differs from all multi-macronucleate congeners either by the number of macronuclear nodules and ciliary rows or by the presence of prominent spines on resting cysts. Both morphological and molecular data support species-level status for S. rectitoratum and S. securiforme. Although S. papilliferum is one of the morphologically most distinct spathidiids due to the presence of oral bulge papillae, it is non-monophyletic. Based on molecular analyses, we suggest reassigning the morphologically transitional S. ascendens and S. polynucleatum, both formerly combined with Epispathidium, to the genus Spathidium. Korean and German populations of Apobryophyllum schmidingeri are highly similar, both morphologically and genetically. The present study highlights the complexity of spathidiid taxonomy, which requires a combination of morphological and molecular approaches to resolve.

www.zoobank.org/urn:lsid:zoobank.org:pub:781F932B-97D1-4B15-9E35-602DC6D3AE67     

HAb18G/CD147真核荧光蛋白的表达及鉴定

构建肝癌相关抗原HAb18G／CD147全长及缺失片段E51（第22—50位氨基酸缺失）的真核荧光蛋白表达载体,进一步研究该基因在肝细胞肝癌发生中的作用。通过PCR及Overlapping PCR的方法获得目的基因．与荧光载体pEGFP—N1双酶切、连接、转化E．coli JM109．构建含有肝癌相关抗原HAb18G／CD147全长及E51的真核荧光蛋白表达载体．并经限制性酶切及序列分析证明插入是否正确。用阳离子脂质体介导转染COS-7细胞,进行瞬时表达;荧光显微镜观察EGFP表达,通过流式细胞术检测蛋白的表达情况,明胶酶谱法鉴定其功能。成功地构建了真核荧光蛋白表达载体pEGFP—N1／HAb18G、pEGFP—N1/E51,并经限制性酶切及序列分析证明外源基因插入正确;流式细胞术鉴定该蛋白表达正确;功能实验证实缺失片段E51不具有诱导成纤维细胞分泌MMPs的功能．结果显示HAb18G／CD147基因第22—50位氨基酸与其刺激成纤细胞分泌MMP和功能有密切关系。实验结果为HAb18G蛋白分子的生物学功能研究奠定了基础。     

Overexpression of HAb18G/CD147 promotes invasion and metastasis via α3β1 integrin mediated FAK-paxillin and FAK-PI3K-Ca2+ pathways

Mechanism of HAb18G/CD147 underlying the metastasis process of human hepatoma cells has not been determined. In the present study, we found that integrin α3β1 colocalizes with HAb18G/CD147 in human 7721 hepatoma cells. The enhancing effect of HAb18G/CD147 on adhesion, invasion capacities and matrix metalloproteinases (MMPs) secretion was decreased by integrin α3β1 antibodies (p<0.01). The expressions of integrin downstream molecules including focal adhesion kinase (FAK), phospho-FAK (p-FAK), paxillin, and phospho-paxillin (p-paxillin) were increased in human hepatoma cells overexpressing HAb18G/CD147. Deletion of HAb18G/CD147 reduces the quantity of focal adhesions and rearranges cytoskeleton. Wortmannin and LY294002, specific phosphatidylinositol kinase (PI3K) inhibitors, reversed the effect of HAb18G/CD147 on the regulation of intracellular Ca²⁺ mobilization, significantly reducing cell adhesion, invasion and MMPs secretion potential (p<0.01). Together, these results suggest that HAb18G/CD147 enhances the invasion and metastatic potentials of human hepatoma cells via integrin α3β1-mediated FAK-paxillin and FAKPI3K-Ca²⁺ signal pathways. Received 5 June 2008; received after revision 16 July 2008; accepted 23 July 2008     

DS18B20在单片机温控系统中的应用

DS18B20新型单总线数字温度传感器集温度测量和A/D转换于一体,直接输出数字量,与单片机接口电路简单。作者介绍了一种基于DS18B20和AT89C2051单片机的温度测量及控制系统的硬件结构及汇编程序设计。     

TC18钛合金多次冲击损伤演化规律

为了探究TC18材料在多次冲击下的损伤演化规律,参考夏比冲击标准设计了三点弯冲击试验件,开展了多次冲击试验。通过动态拉伸试验以及两个应变水平的低周疲劳试验获得了TC18材料的Johnsonson-Cook本构模型、Lemaitre损伤模型中的材料参数,引入修正因子改进了Lemaitre损伤模型,并编写了子程序,基于Johnson-Cook本构模型和改进的Lemaitre损伤模型对试验件进行了多次冲击数值仿真。结果表明,采用改进的Lemaitre损伤模型的有限元仿真结果与试验结果符合较好,TC18材料在多次冲击下损伤变化呈现两个阶段——缓慢累积和迅速增长。     

含有重组人白细胞介素18基因的工程菌的产物表达和鉴定

0引言 1996年,Ushio等[1]从人的肝脏细胞中克隆了人的白细胞介素18(IL-18) cDNA,并在大肠杆菌中得到了表达.IL-18是国际上最新获得的细胞因子[2].