Linking morphology and molecules: integrative taxonomy of spathidiids (Protista: Ciliophora: Litostomatea) from Korea

We isolated seven spathidiids from various terrestrial habitats in Korea: Enchelys megaspinata sp. nov.; Spathidium ascendens Wenzel, 1955; S. papilliferum Kahl, 1930; S. polynucleatum (Foissner et al., 2002) comb. nov.; S. rectitoratum Kahl, 1930; S. securiforme Kahl, 1930 stat. nov.; and Apobryophyllum schmidingeri Foissner and Al-Rasheid, 2007. Their vegetative and resting cyst morphology were studied in vivo and after protargol impregnation. Their phylogenetic relationships were inferred from the analysis of 18S rRNA gene and ITS1-5.8S-ITS2 region sequences. Enchelys megaspinata is nested within the Semispathidium breviarmatum cluster. It differs from all multi-macronucleate congeners either by the number of macronuclear nodules and ciliary rows or by the presence of prominent spines on resting cysts. Both morphological and molecular data support species-level status for S. rectitoratum and S. securiforme. Although S. papilliferum is one of the morphologically most distinct spathidiids due to the presence of oral bulge papillae, it is non-monophyletic. Based on molecular analyses, we suggest reassigning the morphologically transitional S. ascendens and S. polynucleatum, both formerly combined with Epispathidium, to the genus Spathidium. Korean and German populations of Apobryophyllum schmidingeri are highly similar, both morphologically and genetically. The present study highlights the complexity of spathidiid taxonomy, which requires a combination of morphological and molecular approaches to resolve.

www.zoobank.org/urn:lsid:zoobank.org:pub:781F932B-97D1-4B15-9E35-602DC6D3AE67     

TC18钛合金多次冲击损伤演化规律

为了探究TC18材料在多次冲击下的损伤演化规律,参考夏比冲击标准设计了三点弯冲击试验件,开展了多次冲击试验。通过动态拉伸试验以及两个应变水平的低周疲劳试验获得了TC18材料的Johnsonson-Cook本构模型、Lemaitre损伤模型中的材料参数,引入修正因子改进了Lemaitre损伤模型,并编写了子程序,基于Johnson-Cook本构模型和改进的Lemaitre损伤模型对试验件进行了多次冲击数值仿真。结果表明,采用改进的Lemaitre损伤模型的有限元仿真结果与试验结果符合较好,TC18材料在多次冲击下损伤变化呈现两个阶段——缓慢累积和迅速增长。     

DS18B20在单片机温控系统中的应用

DS18B20新型单总线数字温度传感器集温度测量和A/D转换于一体,直接输出数字量,与单片机接口电路简单。作者介绍了一种基于DS18B20和AT89C2051单片机的温度测量及控制系统的硬件结构及汇编程序设计。     

鹿角菜18S rDNA序列分析及其系统发生分析

通过制备鹿角菜DNA,PCR扩增得到鹿角菜18S rDNA序列.测序拼接后全长1733 bp,碱基A、T、C、G含量分别为25.45%、26.72%、26.72%、21.12%,序列已提交Gene Bank登录号为GQ433994.该序列与NCBI数据库中其他褐藻18S rDNA序列比对后,得到可变碱基位点184个,简约信息位点161个,单碱基变化位点23个.转换碱基值Si为44,颠换碱基值Sv为30,转换颠换比值R约为1.5.NJ法构建的系统发生树显示18S rDNA在褐藻门中具有保守性,可用于辅助传统分类.PLACE数据库预测发现在鹿角菜18S rDNA保守区有多个与水分胁迫、光诱导、Ca2+信号传导等相关转录元件,这表明18S rDNA可能参与细胞重要调控途径.     

基于DS18B20的分布式温度监测系统的设计

设计了基于DS18B20传感器的分布式温度监测系统,给出具体应用电路,并重点介绍了DS18820的读写方法.软件设计基于嵌入式实时操作系统uCOSⅡ,保证了系统实时性和可扩展性,在中央空调实验系统中应用该系统,达到预期的技术指标.     

Sr18菌杀线虫代谢产物30L罐发酵放大条件的优化

在原有摇瓶及5L罐发酵的基础上,对线虫生防菌株Sr18杀线虫代谢产物30L罐发酵放大的工艺条件进行了优化,确定了30L罐发酵的工艺参数为：一级种子静置培养24h,接种体积分数为4％,通气量1：0．35～1．0,溶氧量20％～30％,发酵周期48h左右．在优化的发酵工艺条件下,经30L罐发酵放大培养所得发酵滤液1／2浓度的杀线率可达95％．     

Positive correlation between δ~(18)O in monsoon precipitation and atmospheric wind speed

Analyzed results of the atmospheric wind speedand stable isotopic data (δ^18O) in summer precipitation atBangkok, Bombay, New Delhi, Kunming and Lhasa, the IAEA/WMO stations, indicate that δ^18O in monsoon pre-cipitation correlate positively to wind speed and that thereexists a monsoonal vapor layer over these monsoon-controlled areas during monsoon seasons. The isotopic ex-change happens between monsoon vapors and failing rain-drops in the layer, resulting in this correlation between δ^18O and wind speed. This suggests that wind speed is probablyone of key factors affecting the δ^18O variation besides air temperature and rainfall in the southwest monsoon domain.     

基于nRF401芯片的温度无线数据采集系统设计

介绍了基于nRF401芯片的温度无线数据采集系统的设计.给出了无线数据采集系统的硬件具体电路及通信协议的具体格式,上位机采用查询的方式对多个测温点进行查询.系统具有数据传输速度快、可靠性高、功能易扩展等特点,适用于多种工业领域.     

基于DS18B20的温度检测系统

本文介绍了DS18B20智能温度传感器的性能特点,内部机构、测温原理,给出了DS18B20与单片机构成的测温系统的硬件电路及软件流程。