45#钢在含有微米级二硫化钼的18#齿轮油中的摩擦磨损性能

通过45#钢在二硫化钼质量分数分别为0％,0．5％,1％,1．5％,2％的18#齿轮油润滑的5种工况下,进行不同载荷的滑动摩擦试验,比较其摩擦磨损性能并分析磨损机制。结果表明,载荷为300N时,二硫化钼质量分数为2％的18#齿轮油中45#钢磨损量比其在基础油工况下降低了43．5％,摩擦系数降低了33．1％。二硫化钼质量分数为2％时18#齿轮油的抗摩擦磨损性能最好。     

一种基于定量驱动策略和18O标记方法的比较蛋白质组学分析平台

定量蛋白质组学技术是一种高通量的蛋白质分析技术, 有望大大推动生物学和医学研究的发展. 最近提出了一种基于定量驱动的定量蛋白质组学分析新策略, 它采用定量和定性分析分开的模式, 先通过液相色谱一级质谱(LC-MS)定量分析确定差异肽段, 然后针对这些差异肽段再做液相色谱串联质谱(LC-MS/MS)定性分析, 最后通过定性和定量数据的匹配来获得差异蛋白结果. 这种策略被证明可以针对性地鉴定差异肽段, 增加差异蛋白的检测个数. 我们基于该策略和18O标记方法建立了一种新的定量蛋白质组学分析平台. 研究证明了该分析平台在定量蛋白质组学研究中的可行性. 本文还根据此研究在理论上提出了一种新的MS/MS分析的数据依赖模式, 即实时定量依赖的MS/MS离子选择模式.     

Characteristics of δ 18O in precipitation over Eastern Monsoon China and the water vapor sources

Monsoon circulation is an important carrier of water vapor transport, and it impacts the precipitation of the monsoonal regions through the constraints and controls of large-scale water vapor transport and distributions as well as the water vapor balance. An overall research on stable Hydrogen and Oxygen isotopes in precipitation over Eastern Monsoon China could benefit a compre-hensive understanding of the monsoonal precipitation mechanism. Seventeen field stations of the Chinese Network of Isotopes in Precipitation (CHNIP) have been selected to collect monthly composite precipitation samples during the years 2005―2006. Components of δD and δ¹⁸O have been analyzed to achieve the spatiotemporal distributions. The established Local Meteoric Water Line δD=7.46 δ¹⁸O+0.90 based on the 274 obtained monthly samples could be treated as isotope input functions across the region, due to basically reflecting the specific regional meteorological conditions over Eastern Monsoon China. The δ-value de-pleted from coastal to inner area. In Southern China and Northeastern China there were typical periodic patterns of δ¹⁸8O. Differ-ent dominant affecting metrological factors have been raised with different regions. From south to north, the temperature effect of δ¹⁸18O enhanced, while the amount effect changed from existing at an all-year-scale in Southern China to being only remarkable during the main rainy seasons in North China and Northeastern China. Main geographical controls varied from altitude in South-ern China and North China to latitude in Northeastern China. Furthermore, δ¹⁸O had an implication of advance and retreat of the monsoon as well as rainfall belt transfer δ¹⁸O was also a tracer for the movement path of typhoon and tropical storms.     

交通运输系统中的温度测量电路的设计

本系统主要由温度传感器及单片机组成,着重介绍了温度传感器DS18B20的性能特点、内部结构、测温原理及其与单片机的连接方式。     

CAN总线单片机PIC18F258在汽车电子控制单元中的应*用

以集成CAN控制器的PIC18F258单片机为例,介绍了汽车电子控制单元的设计方法,给出了系统主要硬件结构和软件设计流程。该电路具有硬件设计简单、可靠性高、实时性强等特点,为基于PIC18F258微处理器的CAN通信电子控制单元的开发提供了一定的科学依据。     

单片机C与汇编混合编程在DS18B20温度采集中的应用

在嵌入式程序设计中,C语言和汇编语言是两种应用最广泛的编程语言,它们各有突出的优点。本文中详细介绍了这两种编程语言混合调用的方法,并以DS18B20温度采集系统为例,在程序设计中灵活运用这两种编程语言,使程序简洁而高效,对学生学习、运用单片机C语言与汇编语言的混合调用,解决实际问题具有一定的参考价值。     

重组鸡IL-18与新城疫HN基因融合蛋白在毕赤酵母中表达条件的优化

为探索ChMIL18-HN在毕赤酵母中最适宜的表达条件,采用小型摇瓶培养系统,通过对重组毕赤酵母菌X-33/pPICZαA-ChMIL18-HN在不同诱导时间、培养液pH值、甲醇诱导剂剂量及培养温度的条件下表达目的蛋白的分析,以对其表达条件进行优化。结果表明:重组菌X-33/pPICZαA-ChMIL18-HN在pH6.5,甲醇诱导浓度为1.5%,培养诱导温度为30℃的条件下诱导156h,目的蛋白的表达量最高。     

Oxygen and hydrogen isotopic compositions of coexistent minerals of the Tasigake alkali granite pluton, northern Xinjiang: constraints upon the cause of18OD depletion and the18O/16O exchange kinetics

Oxygen and hydrogen isotopic compositions have been determined of three coexistent mineral triplets of alkali feldspar, quartz, arfvedsonite of 11 samples collected from the Tasigake alkali granite pluton, Ulungur River region, northern Xinjiang. Isotopic exchange with meteoric water during subsolidus cooling caused strong¹⁸O-D depletion and the remarkable nonequilibrium¹⁸O/¹⁶O relation between coexistent minerals.¹⁸O/¹⁶O exchange kinetic effects of the three minerals are simultaneously and consistently modelled. Exsolution and microtextural reorganization of alkali feldspar, and infiltration of water have been mutually facilitated by each other. Shallow intrusion, and circulation of meteoric water throughout the pluton cool it effectively. As a result, the “heat engine” is rapidly extinguished, and the nonequilibrium¹⁸O/¹⁶O relation is frosen.     

先兆子痫患者血清IL-18和IL-12的定量检测及其在发病机制中的作用研究

应用ELISA方法定量检测了20例正常未孕女性,20例健康妊娠孕妇,11例轻度、25例中度以及16例重度先兆子痫患者血清中IL-18与IL-12的含量。应用流式细胞术检测患者PBMC中Th1/Th2型细胞亚群的频率。健康妊娠孕妇血清中IL-18含量高于未孕女性(P<0.001),而IL-12的含量则要低于未孕女性(P=0.001)。先兆子痫患者血清中IL-18含量高于未孕女性(P<0.001),但与健康妊娠者无差别。轻、中度先兆子痫患者血清中IL-12含量低于未孕女性(P<0.05),中、重度先兆子痫患者血清中IL-12含量高于健康妊娠孕妇(P<0.05)。健康孕妇的IL-18/IL-12比值明显高于未孕女性,随先兆子痫病情严重程度,IL-18/IL-12比值逐渐降低且均低于正常妊娠孕妇。外周血中IL-2/IL-4的分泌比值及IFN-γ/IL-4的分泌比值,在健康未孕女性与健康妊娠孕妇组间无明显的统计学上的差异,但重度先兆子痫患者明显高于健康妊娠孕妇(P<0.01)。先兆子痫患者外周血Th1/Th2细胞亚群比值高于健康妊娠组,且与血清中IL-18/IL-12比值呈负相关(r=-0.535,P<0.001)。正常妊娠孕妇血清中IL-18的增加与IL-12的减少可能诱导Th2呈优势表达;而先兆子痫患者血清中IL-18与IL-12的同时增加可能促使Th1呈优势表达。     

生物芯片中PCR温控系统的研究

聚合酶链反应在温度控制系统(PCR温控系统)中对反应时间和集成化设计提出了严格的要求,因此聚合酶链反应的温度控制系统必须进行实时监控,而且易于集成到一体化芯片上.本文设计了PCR的温度控制系统,并编写了上位机监视软件,为加热器的选型提供了直观的参考.