上消化道病内镜微波组织凝固疗法的临床应用与评价

本文报告应用内镜微波组织凝固疗法(EMCT)治疗胃息肉、胃癌、消化性溃疡三种疾病21例、39次治疗。提出了溃疡面搔刮加微波凝固治疗难治性溃疡、及EMCT联合内镜下局部注射化疗治疗进展期胃癌等方法。胃息肉13例27枚及Ⅱa型早期胃癌1例与消化性溃疡3例全部达到治愈。85.2％的胃息肉EMCT一次治疗成功,Ⅱa型早癌一次成功,消化性溃疡2例一次成功。进展期胃癌4例均达到肿块缩小、狭窄的胃腔扩大与自觉症状改善的效果。鉴于疗效显著,认为三种疾病均适用本疗法;并就EMCT的适应症、使用限度和治疗目的作了讨论;展望了以EMCT作为早期胃癌经内镜根治疗法手段之一的远景。     

Peptide aptamers: exchange of the thioredoxin-A scaffold by alternative platform proteins and its influence on target protein binding

Peptide aptamers have emerged as powerful new tools for molecular medicine. They can specifically bind to and functionally inactivate a given target molecule under intracellular conditions. Typically, peptide aptamers are generated by screening a randomized peptide expression library, displayed from the Escherichia coli thioredoxin A (TrxA) protein. Here, we transferred peptide moieties from defined TrxA-based peptide aptamers to alternative scaffold proteins, such as the green fluorescent protein and staphylococcal nuclease. Yeast and mammalian two-hybrid assays as well as in vitro binding analyses show that the TrxA scaffold can be a major determinant for the binding of peptide aptamers. In addition, we demonstrate that TrxA can correctly display peptide sequences that correspond to the binding domains of natural interaction partners. Therefore, sequence analyses of TrxA-based peptide aptamers, isolated by two-hybrid screening from randomized expression libraries, should also be useful to find cellular binding partners for a given target protein, by homology. Received 1 August 2002; received after revision 17 September 2002; accepted 19 September 2002 RID="*" ID="*"Corresponding author.     

Progress in non-viral gene delivery systems fabricated via supramolecular assembly

Gene delivery systems are one of key issues that limit the development of gene therapy. The novel non-viral gene delivery systems fabricated via supramolecular assembly have begun to show increasing promising and applications in gene therapy due to its suitable nanometric size,controllable structure and excellent biocompatibility. In this review, the fundamental and recent progress of non-viral gene supramolecular assembly is reviewed. Artificial viruses the future direction of non-viral gene delivery systems are also described.     

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Vectors pose most pivotal problem of gene therapy[1]. Because of the high transfection efficiency both in vitro and in vivo, the viral vector has been employed in 70% clinical trials of gene therapy (http://www.wiley.co.uk/ genmed/clinical). However, thei…     

BNCT物理剂量Monte Carlo程序系统——MCDB及算法

硼中子俘获治疗(BNCT,boron neutron capture therapy)规划软件系统MCDB(Monte Carlo dosimetry inbrain),包括医学前处理、Monte Carlo物理剂量计算和后处理。采用中心点方法确定网格的材料和密度,并自动生成Monte Carlo输入文件。MCDB借鉴并发展了一套网格几何下的快速粒子径迹算法,取得了与MCNP程序一致的剂量计算结果,计算速度较MCNP程序提高2.7~3.5倍。MCDB可进行并行计算,具有线性加速比,能够满足BNCT临床对计算时间和精度的要求。     

Polarization of immunity induced by direct injection of naked sequence-stabilized mRNA vaccines

In the context of developing a safe genetic vaccination strategy we tested and studied globin-stabilized mRNA-based vaccination in mice. This vaccination strategy has the advantages of genetic vaccination (easy production, adaptability to any disease and inexpensive storage when lyophilized), but not the drawbacks of DNA vaccination (long-term uncontrolled expression of a transgene, possibility of integration into the host genome and possible induction of anti-DNA antibodies). We report here that injection of naked -globin untranslated region (UTR)-stabilized mRNA coding for -galactosidase is followed by detectable translation in vivo. In addition, we show that such a vaccination strategy primes a T helper 2 (Th2) type of response which can be enhanced and shifted to a Th1-type immune response by application of recombinant granulocyte/macrophage colony-stimulating factor 1 day after mRNA injection. Our data demonstrate that the administration of globin UTR-stabilized mRNA is a versatile vaccination strategy that can be manipulated to fit the requirement of antiviral, antibacterial or antitumor immunity.Received 14 June 2004; received after revision 19 July 2004; accepted 9 August 2004     

拯救灵魂:穆旦宗教选择的精神指向

作为一个现代知识分子,诗人穆旦有着"自我折磨"与"折磨人"的双重人格品质,他在对中国现实的深入思考中敏锐地洞察到:在中国人身上有着一种沉疴已久的精神疾病--"灵魂饥饿",这是传统的信仰价值体系崩溃后,新的信仰价值体系未曾建立时出现的一种病症.穆旦针对"灵魂饥饿"这一现实,通过对中外文化的对比考察,找到了一条救治这一精神疾病的药方--基督信仰,借以医治自己和别人.     

我国古代"情志相胜,以情胜情"的心理疗法及其对现代心理治疗的影响

我国中医学源远流长,其"情志相胜,以情胜情"的心理治疗理念、相应的具体治疗方法及案例,对于分析现代人的心理疾病、并针对性地进行临床治疗具有重要的作用.     

RNA-dependent RNA polymerase encoded by hepatitis C virus: biomedical applications

The hepatitis C viruses (HCVs) are a group of small enveloped RNA viruses that have been viewed as a leading cause of chronic hepatitis in humans. Infections by HCV represent a serious global health problem, because millions of people worldwide are infected and no efficient treatment is available at the present time. Since HCV was identified in 1989, considerable effort has been devoted to the discovery and development of novel molecules to treat HCV-related diseases. One of the approaches is the development of novel inhibitors that interrupt the normal functions of HCV NS5B, an RNA-dependent RNA polymerase essential to HCV replication. This review summarizes recent advances in the biochemical and structural understanding of HCV NS5B polymerase as well as in the development of antiviral agents targeting this important enzyme. Received 19 March 2002; received after revision 23 April 2002; accepted 23 April 2002     

家庭心理治疗理论研究综述

在系统论的观念影响下,心理治疗理论越来越倾向于以系统的观点来指导临床治疗.依据"系统学"的观念,家庭心理治疗就是将个体置于家庭这一系统之中进行分析和治疗.其方法主要包括结构性家庭治疗、系统性家庭治疗、行为家庭治疗和策略性家庭治疗等.