基于UML的时空建模

现实世界中存在大量的时空数据,时空数据的管理对时空建模提出了更高的要求,因此成为当前许多领域的研究热点.UML作为众多领域广泛使用的统一建模语言,基于UML的时空扩展模型也成为许多领域时空建模的重要形式.为此,本文提出一种时空UML模型,该模型能够表示与处理时空对象的多种时空拓扑关系(包括RCC-8空间和Allen-13时态拓扑关系)以及它们之间的复杂语义关系(例如关联、聚合、泛化和依赖);同时给出了时空UML的形式化表示形式和语义解释方法.最后结合地籍变更的实例说明了所提方法的可行性和实用性.     

三种端元气δ13C1-Ro模型建立与应用

端元气δ¹³C₁-R_o模型是天然气成因判识、气源对比及混源气定量判识的重要工具。为了满足Ⅱ型有机质生成天然气识别及评价的需要,根据母质类型将有机热成因天然气划分为I、Ⅱ和Ⅲ型等3种端元气,并利用126组实测天然气数据得到3种端元气的δ¹³C₁-R_o模型。I型,δ¹³C₁=27.55 lg R_o-47.20;Ⅱ型,δ¹³C₁=25.55 lg R_o-40.76;Ⅲ型,δ¹³C₁=48.77 lg R_o-34.10（R_o < 0.9%）,δ¹³C₁=22.42 lg R_o-34.80（R_o ≥ 0.9%）。为了方便模型应利用建模样品的δ¹³C₁、δ¹³C₂数据,绘制了三端元气及其混源气的别图版。根据该图版,济阳拗陷坨165、车571与孤东9井天然气分别为I和Ⅱ型端元气混源、Ⅱ型端元气与I型端元气。     

IL-6对大鼠脑缺血-再灌注后海马IL-1R的影响

目的:探讨脑缺血-再灌注损伤(brainischemiareperfusioninjury)过程中白细胞介素-6 Interleukin-6, (IL-6)对CNS中神经元保护作用的机制及其与炎前细胞因子白细胞介素-1 Interleukin-1,IL-1)之间的关系,为 (研究脑缺血-再灌注损伤的机制提供实验和相关方面的理论基础.方法:采用四动脉暂时性阻断的方法将26只Wistar大鼠制成全脑缺血-再灌注的实验动物模型,并将大鼠分成两组:即单纯的脑缺血-再灌注对照组(n= 9)以及实验组(n = 17),两组大鼠手术前2h分别经侧脑室注入10 L生理盐水和等量的IL-6,然后采用免疫组织化学方法观察缺血-再灌注过程中大鼠海马神经元白细胞介素-1受体(Interleukin-1 receptor,IL-1R)免疫反应性的变化并进行相关的统计学分析.结果:大鼠全脑缺血-再灌注4h后海马CA1、 区IL-1R免疫反应CA3性显著增加,表现为:IL-1R免疫反应阳性细胞数显著增加、着色明显加深.结论:IL-6作为一种神经保护因子在大鼠脑缺血-再灌注损伤的病理过程中,可能通过抑制CNS中神经元的炎症因子IL-1R的表达来实现其对CNS的营养和保护作用.     

肝再生细胞周期中增殖启动及其调控研究

肝再生细胞周期中增殖启动及其调控是一个研究热点,到底是何种因素及其涉及到的信号传导途径诱导了Go期肝细胞的启动仍然是一个谜.本文主要以JNK、TNF-α、IL-6、一氧化氮等因子及其可能参与的调控途径综述了肝再生启动的一些最新研究进展.     

哮喘小鼠骨髓DC细胞因子mRNA的分析

通过体外诱导和扩增小鼠骨髓来源的树突状细胞(DC),研究其中的细胞因子mRNA表达,探讨DC在哮喘中可能的作用机制.为此,利用rmGM-CSF和rmIL-4体外诱导骨髓细胞分化为DC,采用多探针模板的核酸酶保护分析检测骨髓来源的DC中细胞因子mRNA的转录.结果表明,在两种细胞因子的作用下,从骨髓中可以诱导出大量的DC;核酸酶保护分析显示骨髓来源的DC中有IL-13,IL-9和IL-3mRNA的转录,并且哮喘组表达IL-13mRNA和IL-9mRNA的相对水平与对照组相比有明显的差异性,P<0.05;而IL-3mRNA的表达在两组鼠中的表达水平没有差异性,P>0.05.可以认为DC在哮喘的形成和发展中起重要作用,提示了靶向于DC的治疗可能成为哮喘治疗的新目标.     

射击运动员比赛前后生理应激的初步研究

观察射击运动员比赛前后血清内啡肽、血清皮质醇和泌乳素以及IL-6的水平.发现射击比赛前,运动员在应激状态下,激素和炎症因子水平升高,有助于提高运动员应变能力,射击后激素水平上升不明显主要是减少应激损害.     

Mitogenic activity of selenoorganic compounds in human peripheral blood leukocytes

Summary A variety of organoselenium compounds were originally described as antiinflammatory, antioxidant or glutathione-peroxidase-like agents, and as inhibitors of prostaglandin and leukotriene synthesis. Recently, the compounds have also been found to be inducers of interferon gamma and tumor necrosis factor in human peripheral blood leukocytes (PBL). We evaluated the effects of bis [2-(N-phenylcarboxamido)phenyl] diselenide and Ebselen^®; 2-phenyl-1,2-benzisoselenazol-3(2H)one, on the incorporation of tritiated thymidine into the DNA of PBL cultured in vitro. Both compounds were mitogenic and this effect was correlated with the expression of interleukin 2 receptor in T-lymphocytes. Therefore, we suggest that the selenoorganic compounds may induce mitogenic cytokines.     

Interleukin-17 stimulates inducible nitric oxide synthase-dependent toxicity in mouse beta cells

The influence of the proinflammatory cytokine interleukin (IL)-17 on inducible nitric oxide (NO) synthase (iNOS)-mediated NO release was investigated in the mouse insulinoma cell line MIN6 and mouse pancreatic islets. IL-17 markedly augmented iNOS mRNA/protein expression and subsequent NO production induced in MIN6 cells or pancreatic islets by different combinations of interferon-γ, tumor necrosis factor-α, and IL-1β. The induction of iNOS by IL-17 was preceded by phosphorylation of p38 mitogen-activated protein kinase (MAPK), and inhibition of p38 MAPK activation completely abolished IL-17-stimulated NO release. IL-17 enhanced the NO-dependent toxicity of proinflammatory cytokines toward MIN6 cells, while IL-17-specific neutralizing antibody partially reduced the NO production and rescued insulinoma cells and pancreatic islets from NO-dependent damage induced by activated T cells. Finally, a significant increase in blood IL-17 levels was observed in a multiple low-dose streptozotocin model of diabetes, suggesting that T cell-derived IL-17 might be involved in NO-dependent damage of beta cells in this disease. Received 14 June 2005; received after revision 17 September 2005; accepted 21 September 2005     

A Co—expression System Based on Phage and Phagemid to Select Cognate Antibody—antigen Pairs in vivo

A modified selectively-infective phage(SIP) is developed to facilitate the selection of interacting antibody-antigen paris from a large single-chan antibody(scFv)library in vivo.The system is constructed with a modified helper phage M13KO7 and phagemid pCANTAB 5E.The antigen fused to the C-terminal of N1-N2 domain and the scFv to the N-terminal of CT domain of the gIIIp of filamentous phage are encoded on the phage and phagemid vectors respectively.The phages produced by co-transformants restore infectivity via interaction between antigen and antibody fusions in the cell periplasm.In a model system,the scFv fragment of the anti-hemagglutinin 17/9 antibody and its corresponding antigen are detected in the presence of a 10^5 fold excess of a non-interacting cotrol paris,which demonstrates this system to be very sensitive and facile to screen a large single-chain antibody library.     

ZGMn13钢的韧脆转化现象

通过系列冲击试验,对高锰钢的低温韧性进行了研究.并对其断口形貌进行了观察.研究结果表明高锰钢具有明显的韧脆转化现象;其冲击断口微观形貌由室温时的韧窝断口逐渐变为低温下的塑性沿晶断口.