基于实体用户界面的智能手机直觉化交互方式

以智能终端上的交互方式为研究内容,针对智能手机的有形实体交互问题,提出智能手机上的直觉化视窗式交互方法,解决移动终端上由于沿用桌面式图形用户界面交互范式而造成的操作低效与不便的问题,设计并实现了一种基于实体用户界面的手机读图系统Emagn,最后通过实验验证了该方法在交互效率与认知上较传统触摸手势交互方法均有所提高。     

人类体外受精助孕治疗中多原核受精的相关因素分析

收集2011年7月至2012年11月在广西生殖医学研究中心行体外受精助孕治疗的456例患者资料,将观察到多原核受精的235例作为多原核受精组,未观察到多原核受精的221例作为无多原核受精组,比较两组间基础卵泡刺激素(基础FSH)、黄体生成素(LH)水平、窦卵泡数(AFC),促性腺激素(Gn)总剂量及天数,HCG(人绒毛膜促性腺激素)日血清雌二醇(E2)水平、HCG日血清孕酮(P)水平、HCG日血清黄体生成素(LH)水平,HCG日大卵泡(≥14mm)数,获卵数,受精密度等助孕治疗相关指标的差异,分析人类体外受精中多原核受精的影响因素。结果提示,Gn用量小,HCG日血清E2水平较高,卵泡数及获卵数较多,受精率较高的周期中出现至少1个多原核受精合子的可能性比较大。     

城市交通噪声及其危害

噪声,是人们不愿意听的声音。环境噪声影响人们的工作、学习、谈话、思考、休息和睡眠。长此以往,环境噪声不仅危害人们的身心健康,甚至折损人的寿命。城市交通噪声,对环境和人体健康影响最为普遍、显著。目前,北京市交通要道的噪声水平,已经超过国家规定的环境噪声控制标准,需要采取控制措施。     

Evidence of fire use of late Pleistocene humans from the Huanglong Cave, Hubei Province, China

Since 2004, three excavations have been carried out at a late Pleistocene human fossil site of Huanglong Cave in Yunxi County, Hubei Province of China, which unearthed seven human teeth, dozens of stone tools, mammal fossils and other evidence indicating human activities. During the third excavation in 2006, in the same layer as the human teeth, we found some patches of black materials embedded in the deposit. We doubted that this black deposit layer is the remains of burning or even human use of fire at the cave. To further explore the possibility of human fire use at the Huanglong Cave, we examined samples directly taken from the black deposit layer and compared them with samples taken from several places in the cave using three methods: micromorphology, element content determination and deposit temperature analysis. Our results indicate that the contents of carbon element in the black deposit reach 64.59%―73.29%. In contrast, contents of carbon element of the comparative samples from other parts in the cave are only 5.82%―9.49%. The micromorphology analysis of the black deposit samples reveals a plant structure like axial parenchyma, fibrocyte, uniseriate ray and vessel. High-temperature magnetic susceptibility measurements suggest that the stratum possibly underwent a high temperature in the nature. Based on these lab analyses, we are sure that the black layer in the Huanglong Cave is the remains of fire and combustion did occur in the cave 100000 years ago. Taking other evidence of human activities found in the Huanglong Cave into consideration, we believe that the evidence of fire from the Huanglong Cave was caused by the human activities of controlled use of fire.     

践行科学发展观 提升高校图书馆人力资源的管理

本文从科学发展观的角度,对高校图书馆人力资源的开发和管理提出了新的要求,强调以人为本,注重科学的观点;并阐述了以科学发展观推动高校图书馆人力资源开发和管理的策略。     

Production of human lysozyme-transgenic cloned porcine embryos by somatic nuclear transfer

Due to their physiology and organ size, pigs have significant potential as human disease models and as organ transplantation donors. Genetic modification of pigs could provide benefits for both agriculture and human medicine. In this study, five fetal pig fibroblast cell lines from two species (Wuzhishan and Landrace pigs) were transfected using double-marked human lysozyme (HLY) plasmids (pBC1-HLY-GFP-NEO) by a liposome-mediated method. The ratio of green fluorescent protein (GFP)-expressing cells was >95% in sw7, sw8, slw3 and slw6 cell lines, but only 49.3% in slw9 cells. Cells from the four highly transgenic lines were used as nuclear donors to construct embryos, which were then cultured after fusion and activation by electric stimulation. The rate of cleavage was 76.7%, 48 h after activation. After 7 days, 18.5% of cleaved eggs had developed to the blastocyst stage and 93.3% of blastocysts were GFP-positive. These results indicate that transgenic fetal pig fibroblast cell lines could be obtained by a liposome-mediated method, though the transfection efficiency varied between cell lines. Reconstructed embryos derived from transgenic cells could successfully develop into blastocysts, most of which were GFP-positive.     

试论电视纪录片的“人文关怀”及其表现形态

关于电视纪录片的“人文关怀”一直是受评论家关注的现象,但也大都是局限于纪录片本身来谈的,而本文作者力图将其放在社会文化价值观建构的背景下进行分析。文章着重讨论了纪录片人文关怀的内容特征,社会意义及其多样化的表现形态。     

绒毛膜促性腺激素对人外周T淋巴细胞转化的影响

目的;探讨人绒毛膜促性腺素（human chorionic gonadotropin,HCG）在神经内分泌－免疫网络中的作用。方法：应用同位素示踪法体外观察HCG以人外周T淋巴细胞转化增殖反应的影响。结果：HCG浓度在7．5－125U／mL时对植物凝集索刺激的T淋巴细胞转化增殖反应有显著抑制作用（P＜0．001）。结论：HCG作为一种负性调节因子,通过对T淋巴细胞的抑制作用,可能在神经内分泌－免疫调节网络中起重要作用,不仅参与妊娠时机体对胎儿的免疫相容机制,而且与肿瘤患者体风的免疫抑制机制有关。     

类人弹性蛋白在大肠杆菌中的高效表达

类人弹性蛋白是由VAPGVG六肽重复序列构成的多肽。研究通过全基因合成获得上游引入NcoI和下游引入XhoI限制性内切酶位点的[(VAPGVG)3S]4基因编码序列,通过定向递归连接技术在pMD19-T simple克隆载体中构建[(VAPGVG)3S]32基因编码序列。利用NcoI和XhoI双酶切从重组克隆载体中回收[(VAPGVG)3S]32(ELPs)基因编码序列,在T4 DNA连接酶作用下与NcoI和XhoI双酶切处理的线性化载体DNA分子pET28a(+)重组,重组混合物转化大肠杆菌Top10感受态细胞。从添加有50μg·mL-1卡那霉素的LB平板上随机挑取37℃培养过夜的转化克隆,碱法小量抽提质粒DNA,经酶切及序列测定筛选出序列正确的重组质粒DNA转化大肠杆菌BL21(DE3)感受态细胞。从添加有50μg·mL-1卡那霉素的LB平板上随机挑取37℃培养过夜的转化克隆至新鲜配制的TB液体培养基中,于37℃、200 r·min-1培养至OD600约为0.60 h,添加终浓度为100μg·mL-1的IPTG诱导类人弹性蛋白表达3 h,SDS-PAGE凝胶电泳及Western blotting分析表明,该研究成功构建了类人弹性蛋白原核表达载体,在IPTG诱导作用下以可溶性状态高效表达。灰度扫描分析显示,重组类人弹性蛋白经IPTG诱导表达3 h后,可达宿主细胞可溶性蛋白的28.3%。该工作为以大肠杆菌为表达宿主,大量制备类人弹性蛋白用于生物医学材料奠定了良好的基础。     

水溶液中人胆汁糖蛋白与碳酸钙的结合研究

利用付里叶变换红外光谱 (FT -IR) ,结合减法、二阶导、退卷积和曲线拟合等先进光谱技术及ICP -AES分析方法 ,对人胆汁中提取的糖蛋白 (HBG)和碳酸钙 (CaCO3)反应后的滤液进行了研究。结果表明 ,HBG和碳酸钙之间存在着络合配位和多形式的氢键作用 ,使两者的微观结构发生了变化 ,形成了水溶性的HBG -碳酸钙复合物。该复合物的形成是生物矿化的基础。