Thrombospondin co-localises with TGFβ and IGF-I in the extracellular matrix of human osteoblast-like cells and is modulated by 17β estradiol

Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10^–10 M–10^–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors.     

抗人绒毛膜促性腺激素（HCG）单克隆抗体的研制和特性分析

以HCG为抗原,免疫BALB/c小鼠,取其脾细胞与小鼠Sp2/0—Ag14骨髓瘤细胞融合,经反复筛选和再克隆化,获得8株能稳定分泌抗HCG特异单克隆抗体的杂交瘤细胞株。对其中6个高效价单抗进行了特异性和抗原决定簇检测,选出两个抗相距较远不同决定簇的单抗(2D_4,4F_4)作进一步特性分析。结果显示:免疫球蛋白亚类鉴定,2D_4为IgGl亚类,4F_4为IgG2a亚类;亲和力常数(K_(affi)),2D_4为5.41±0.94×10~(-10)mol/L,4F_4为1.37±0.33×10~(-9)mol/L。证实该两种单克隆抗体可用于研制有关临床诊断试剂盒。     

人类染色体中的微切割与微克隆技术

微切割、微克隆是分子生物学的一项新技术，它对于基因的标记与分离、DNA文库的重组及遗传图谱的绘制都发挥着重要作用．笔者介绍了这项技术在人类染色体中的应用情况．     

三维人体建模技术探讨

对当今广为应用的线框模型、体模型和曲面模型等传统的三维人体建模方法进行了研究和分析 ,并探讨了基于物理特性的人体建模技术     

现代主义文学唤醒沉睡的人性

中国传统文化的自身缺陷导致了传统文学中人性的缺失,在作家笔下性成了被压抑的对象,人成了道德化、畸形化了的人,人性在文本中得到扭曲。随着西方现代主义文学渐趋渗透,许多文化先驱接受了这一理论,并指导他们的文本创作,打破了传统文本创作的禁区,以一种更理性、更接近人自身的方式书写人性,唤醒了沉睡已久的性意识,从而使文学中对人性的探讨更趋于完善与全面。     

人类基因组计划及后基因组研究概论

综述了人类基因组计划及后基因组研究概况，指出该研究将对21世纪的生命科学和人类生活产生巨大的影响，尤其在医疗保健领域意义深远，并展望了其发展前景．     

大中型企业人力资源管理系统分析与实现

文章以大中型企业为例，阐述了在人力资源系统设计中采用的技术和方法，以及对人力资源系统进行系统总体设计的经验。     

论自然灾害防御的理性措施

人与自然的不协调发展导致了自然灾害发生频次的不断上升。为了保护人居环境、拓展并良化人类生存空间，作者在对自然灾害分类形容的基础上，根据自然灾害发生原因中人为成份不断增加的实际，提出了防御自然灾害的理性措施。     

新马克思主义文艺学:本体论的新拓展--王元骧文学原理的哲学思考之三

本体论的新拓展是王元骧新马克思主义文艺学的组成部分之三,也是王元骧文学原理的哲学思考之三。本文由自然本体论、人类本体论和生命本体论三大系列建构而成。而其中的生命本体论源于康德的人类本体论,是叔本华的意志哲学/生命本体论关于原始欲望、本能冲动的“生命意志”学理。王元骧不仅将它引进新马克思主义文艺学,而且还把狄尔泰的生命哲学美学、叔本华的悲剧理念和尼采的强力意志等合理内核借鉴到新马克思主义文艺学,对以前很少问津的本体论予以新拓展。这是王元骧文学原理的哲学思考之后的超越。     

2002年山东省大气污染造成的经济损失估算

在对2002年山东省大气污染资料调查和统计的基础上，应用环境经济学的原理与方法(人力资本法、市场价值法、机会成本法、资产价值法等)，对大气污染造成的经济损失进行了估算，结果表明2002年山东省大气污染经济损失约为126．379亿，约为当年山东省GDP的1．2％。