用脾内免疫法制备抗人尿激酶单克隆抗体

用常规免疫法制备抗人尿激酶单克隆抗体费时、费样品。本文采用脾内直接注射的免疫方法,利用杂交瘤技术制备了3C_6抗尿激酶单克隆抗体,3C_6单克隆抗体属IgM亚类,主要识别MW 54,000的高分子量尿激酶和MW 33,000的低分子量尿激酶。3C_6单克隆抗体与结构类似的胰蛋白酶和纤溶酶原无交叉反应,与胰凝乳蛋白酶原和胰凝乳蛋白酶有一定程度的交叉反应。     

Big data analysis of the human brain’s functional interactions based on fMRI

The human brain is a huge, complex system generating brain activity. The exploration of human brain function using functional magnetic resonance imaging （fMRI） is a promising method to understand brain activity. However, the complexity of the big data generated by fMRI facilitates the analysis of various levels of human brain activity, such as the distribution of neural represen- tations, the interaction between different regions, and the dynamic interaction over time. These different levels can depict distinct prospects of the human brain activity, and considerable progress has been achieved. In the future, more big data analysis methods combining advances in computer science, including larger-scale computing, machine learning, and graph theory, will promote the understanding of the human brain.     

基于空间视觉掩蔽效应的图像数字水印频域技术

首先分析了人眼视觉系统的特性,提出了一种利用人眼视觉掩蔽效应,以获得较好鲁棒性和不为人眼所感知的数字水印方法。实验结果表明,这一方法具有较好的特性。最后结合实验给出了对彩色图像进行数字水印处理的方法。     

血管生成抑制因子arresten克隆及功能的初步研究

血管生成抑制因子arresten是血管生成及肿瘤生长抑制物.研究发现,将arresten基因重组于质粒pGEX4T1转化入大肠杆菌BL21并用IPTG诱导蛋白表达,可获得分子量为45kDa的目的蛋白条带,arresten蛋白多数以包涵体形式存在,也有可溶蛋白存在于菌裂解液的上清,可溶蛋白可由Sepharose 4B凝胶柱亲和层析柱纯化.在鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)实验中,arresten基因可溶蛋白被证实抑制新生血管生成的效果较好.     

基于价值链视角的电影产业发展策略研究

在消费升级的背景下,中国电影票房收入不断突破历史新高,但中国电影产业仍然处于大而不强的发展阶段.本研究以韩国电影产业崛起的案例为切入点,运用产业价值链理论和结构-行为-绩效(SCP)框架分析了中国电影产业的发展环境,找出了中国电影产业的发展瓶颈.研究发现:人力资本是电影产业的核心竞争力,实现电影产业跨越式发展的关键就在于最大限度地激发人的创造力;电影产业的发展史是一部科学、技术与文化交互融合的演进史,要抓住重大科技创新机遇,运用大数据、云计算、人工智能等新兴科技实现电影产业的弯道超车;电影产业具有多产业跨界融合的特点,产业链条的交互融合是推动电影产业持续发展的重要动力.     

人发中痕量锰的脉冲溶出伏安法测定

<正> 锰在生物化学方面是一个重要的元素,也是在人体中一种必需的痕量元素,但超过正常量时又是典型的有毒元素.缺锰及锰中毒在临床上可引起一系列疾病.目前国内外有关人发中痕量锰的分析方法有中子活化法,原子吸收光谱法,X 光荧光光谱法及电感耦合等离子发射光谱分析法等.未见到用脉冲极谱结合溶出伏安法测定发锰的文献报导.本文采用国产的 F-78型脉冲极谱仪,旋转玻碳汞膜电极为工作电极,用差分脉冲阳极溶出伏安法,以0.01mol/L NaNO_3为支持电解质,测定了人发中痕量锰,并对     

The atmospheric environment—an introduction

The atmosphere is part of the environment with which the human organism is permanently confronted. Epidemiological research investigates the occurrence of effects on morbidity and mortality due to heat, cold, air pollution and changes in the weather. Concentrating on aspects of the environment relevant for medical questions, three major complexes of effects can be discriminated: the complex conditions of heat exchange, the direct biological effects of solar radiation, and air pollution. Biometeorological knowledge can serve to assess the atmospheric environment, and can also be of help in the field of preventive planning, to conserve and develop the climate as a natural resource with regard to man's health, well-being and performance.     

Spontaneous rhythmic contractions of human saphenous veins isolated from old subjects are sensitive to cyclooxygenase inhibitors

Summary Spontaneous rhythmic contractions were observed in some preparations of human isolated saphenous veins from old (>60 years) subjects. These contractions were insensitive to adrenergic and histaminergic blockers, but were abolished by the cyclooxygenase inhbitors, aspirin and indomethacin, indicating the participation of endogenous eicosanoids.     

五四启蒙文学思潮论

与五四新文化运动思想启蒙的要求相一致,在文学领域内兴起了一股启蒙文学思潮,它主要表现在五四新文化先驱的文学思想主张上,以周作人"人的文学"观为精神标高。"人的文学"观包蕴两个既相互联系又相互区别的侧面:人道主义和个性主义。这股启蒙文学思潮在五四后半期(1921年以后)分化成现实主义、浪漫主义和现代主义3种文学思潮。     

Persistent 24-h variations of urinary 6-hydroxy melatonin sulphate and cortisol in Antarctica

Summary Bright light (2000–3000 lux) of sufficient intensity to suppress human melatonin secretion, acts as a strong zeitgeber in the entrainment of circadian rhythms in man. In polar conditions, light of this intensity is not experienced for several weeks during the winter. The entrainment of human circadian rhythms, in particular that of melatonin, is clearly of interest in these circumstances. Urinary 6-hydroxy melatonin sulphate (aMT6s) is a good index of melatonin secretion in man. In a limited study of seven male volunteers living on an Antarctic base the overall 24-h rhythm of aMT6s excretion was maintained at four different times of year (spring, summer, autumn and winter) and no significant seasonal effects were noted. Cortisol excretion, appeared to be markedly affected by the season although other factors such as social and environmental stress cannot be discounted. These observations suggest that in the absence of a strong light-dark cycle melatonin production may be entrained by other factors.